桑黄多糖的体外抗氧化作用研究
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摘要
以对DPPH自由基和羟基自由基的清除活性以及还原力为指标,测定桑黄多糖的体外抗氧化作用。结果显示,桑黄多糖具有较强的还原力以及对DPPH自由基和羟基自由基的清除作用,其还原力和自由基清除作用与其浓度呈现量效关系。结果表明,桑黄多糖具有明显的体外抗氧化作用。
The antioxidant effects of the polysaccharide from phellinus baumii in vitro were studied by determining the clearance rate of DPPH radical and hydroxyl radical, and the reduction ability. The results showed that the phellinus baumii polysaccharide had strong reducing ability and scavenging effect on DPPH radical and hydroxyl radical, which exhibited a certain dose-effect relationship with polysaccharide concentration. The results suggested that phellinus baumii polysaccharide had obvious antioxidant effects in vitro.
The antioxidant effects of the polysaccharide from phellinus baumii in vitro were studied by determining the clearance rate of DPPH radical and hydroxyl radical, and the reduction ability. The results showed that the phellinus baumii polysaccharide had strong reducing ability and scavenging effect on DPPH radical and hydroxyl radical, which exhibited a certain dose-effect relationship with polysaccharide concentration. The results suggested that phellinus baumii polysaccharide had obvious antioxidant effects in vitro.
引文
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[3]Park H J,Han E S,Park D K,et al.An extract of phellinus linteus grown on germinated brown rice inhibits inflammation markers in RAW264.7 macrophages by suppressing inflammatory cytokines,chemokines,and mediators and up-regulating antioxidant activity[J].J Med Food,2010,13(6):1468-1477.22
[4]Noh J R,Lee I K,Kim Y H,et al.Antiatherogenic effect of antioxidant polyphenols from phellinus baumii in apolipoprotein E-deficient mice[J].Ann Nutr Metab,2011,2(59):145-153.
[5]曾鹏,黄世荣,刘明明,等.响应面法优化超声波辅助热水提取桑黄多糖的工艺条件[J].蚕业科学,2015,41(5):928-933.
[6]LIU Ming-ming,ZENG Peng,LI Xiao-tong,et al.Antitumor and immunomodulation activities of polysaccharide from Phellinus baumii[J].International Journal of Biological Macromolecules,2016,91:1199-1205.
[7]Hsu B,Coupar I M.,Ng K.Antioxidant activity of hot water extract from the fruit of the Doum palm,Hyphaene thebaica[J].Food Chemistry,2006,98,317-328.
[8]Smirnoff N,Cumbes Q J.Hyroxyl radical scavenging activity of compatible solutes[J].Phytochemi stry,1989,28(4):1057-1060.
[9]李慎新,曹新志,钟俊波,等.几种中草药抗氧化性成分的还原能力及总酚含量比较研究[J].安徽农业科学,2008,36(29):12755-12756.
[10]陈亚洁,时连根.生物多糖药理作用的研究概况[J].蚕桑通报,2017,48(3):15-18,27.
[11]Sultanova N,Makhmoor T,Abilov Z A,et al.Antioxidant and antimicrobial activities of Tamarix ramosissima[J].Journal of Ethnopharmacology,2000,78:201-205.
[12]李小定,荣建华,吴谋成.灰树花多糖PGF-1体外对羟基自由基的抑制作用[J].食品科学,2003,24(7):120-130.
[13]Siddhuraju P,Mohan P S,Becker K.Studies on the antioxidant activity of Indian Laburnum(Cassia fistula L.):a preliminary assessment of crude extracts from stem bark,leaves,flowers and fruit pulp[J].Food Chemistry,2002,79:61-67.
[14]Meir S,Kanner J,Akiri B,et al.Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves[J].Journal of Agricultural and Food Chemistry,1995,43:1813-1817.