铁皮枫斗多糖对小鼠急性酒精性肝损伤的保护作用
|
摘要
采用热水浸提方法提取铁皮枫斗多糖,研究其对小鼠急性酒精性肝损伤的保护作用。通过体积分数50%乙醇溶液灌胃诱导小鼠急性肝损伤,连续给药14 d后,摘眼球取动物血,采用血细胞计数仪测定红细胞(red blood cell,RBC)含量、红细胞比容(hematocrit,HCT)、红细胞平均体积(mean corpuscular volume,MCV)、白细胞(white blood cell,WBC)含量和血红蛋白(hemoglobin,HGB)质量浓度,采用试剂盒测定血清中谷丙转氨酶(aspartate transaminase,ALT)活力、谷草转氨酶(aspartate amino transferase,AST)活力及甘油三酯(triglyceride,TG)含量,取肝脏计算肝脏指数并制备肝匀浆,测定其中超氧化物歧化酶(superoxide dismutase,SOD)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力及TG含量,并对小鼠肝脏进行组织切片观察。结果表明:与空白对照组相比,模型组血液的RBC含量、WBC含量以及血清中的TG含量、ALT活力、AST活力,肝脏指数,肝组织的TG含量、SOD活力和GSH-Px活力具有显著性差异(P<0.05或P<0.01)。相对于模型组,给药(铁皮枫斗多糖)后可显著降低血液的RBC和WBC含量,血清中的TG含量,ALT活力、AST活力,肝脏指数,肝组织的TG含量(P<0.05或P<0.01),提高肝组织的SOD和GSH-Px活力(P<0.05或P<0.01),明显改善肝组织损伤程度。铁皮枫斗多糖对酒精诱导的小鼠急性肝损伤有明显的保护作用。
The hepatoprotective effect of polysaccharides isolated from the dried stems of Dendrobium candidum by hot water extraction was evaluated on alcohol-induced acute liver injury in mice.Mice were intragastrically administrated with 50% alcohol to induce alcohol-induced acute liver injury.Thereafter,the mice were gavaged with the polysaccharides for 14 consecutive days.At the end of the experiment,blood was taken by removing eyeballs,and then the mice were sacrificed with cervical dislocation to harvest their liver tissues.Red blood cell(RBC),haematocrit,mean corpuscular volume,white blood cell(WBC) and hemoglobin were measured using a blood cell counter.Superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) activity and triglyceride(TG) content in liver,as well as alanine transaminase(ALT),aspartate transaminase(AST) and TG levels in serum were measured.Histopathological liver changes were examined.The results showed that RBC and WBC levels,the serum levels of ALT,AST and TG,and SOD,GSH-Px activity and TG content in liver,and liver index significantly differed(P<0.05 or P<0.01) between the control and model groups.Dendrobium candidum polysaccharides significantly reduced liver index,RBC,WBC,ALT,AST and TG levels as well as enhanced the activities of SOD and GSH-Px in the liver of mice suffering from alcohol-induced acute liver injury(P<0.05 or P<0.01).The polysaccharides promoted recovery from hepatic tissue injury.To conclude,this study suggests that Dendrobium candidum polysaccharides have a significant hepatoprotective effect on alcohol-induced acute liver injury in mice.
The hepatoprotective effect of polysaccharides isolated from the dried stems of Dendrobium candidum by hot water extraction was evaluated on alcohol-induced acute liver injury in mice.Mice were intragastrically administrated with 50% alcohol to induce alcohol-induced acute liver injury.Thereafter,the mice were gavaged with the polysaccharides for 14 consecutive days.At the end of the experiment,blood was taken by removing eyeballs,and then the mice were sacrificed with cervical dislocation to harvest their liver tissues.Red blood cell(RBC),haematocrit,mean corpuscular volume,white blood cell(WBC) and hemoglobin were measured using a blood cell counter.Superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) activity and triglyceride(TG) content in liver,as well as alanine transaminase(ALT),aspartate transaminase(AST) and TG levels in serum were measured.Histopathological liver changes were examined.The results showed that RBC and WBC levels,the serum levels of ALT,AST and TG,and SOD,GSH-Px activity and TG content in liver,and liver index significantly differed(P<0.05 or P<0.01) between the control and model groups.Dendrobium candidum polysaccharides significantly reduced liver index,RBC,WBC,ALT,AST and TG levels as well as enhanced the activities of SOD and GSH-Px in the liver of mice suffering from alcohol-induced acute liver injury(P<0.05 or P<0.01).The polysaccharides promoted recovery from hepatic tissue injury.To conclude,this study suggests that Dendrobium candidum polysaccharides have a significant hepatoprotective effect on alcohol-induced acute liver injury in mice.
引文
[1]孙广仁.中医藏象生理学[M].北京:中国医药科技出版社,2002:111.
[2]BRANDON-WARNER E,SCHRUM L W,SCHMIDT C M,et al.Rodent models of alcoholic liver disease:of mice and men[J].Alcohol,2012,46(8):715-725.DOI:10.1016/j.alcohol.2012.08.004.
[3]CRABB D W,GALLI A,FISCHER M,et al.Molecular mechanisms of alcoholic fatty liver:role of peroxisome proliferator-activated receptor alpha[J].Alcohol,2004,34(1):35-38.DOI:10.1016/j.alcohol.2004.07.005.
[4]汤小华.铁皮石斛对肝损伤模型小鼠作用研究[D].温州:温州医学院,2010:20-25.
[5]赵爽,荣成博,张淑曼,等.毛木耳多糖对乙醇性肝损伤的保护作用[J].江苏农业科学,2017(12):142-144.DOI:10.15889/j.issn.1002-1302.2017.12.037.
[6]胡德坤,沈业寿.天麻多糖-2对小鼠四氯化碳肝损伤和酒精肝损伤的保护作用[J].中国中医药信息杂志,2007,14(12):29-31.DOI:10.3969/j.issn.1005-5304.2007.12.014.
[7]印会河,童瑶.中医基础理论[M].2版.北京:人民卫生出版社,2007:135-135.
[8]郝杰,查学强,鲍素华,等.霍山石斛不同分子量多糖体外抗氧化研究[J].食品科学,2009,30(15):94-98.DOI:10.3321/j.issn:1002-6630.2009.15.021.
[9]邱现创,赵宁,李晨,等.铁皮石斛多糖提取工艺优化及对果蝇抗氧化能力的影响[J].食品科学,2018,39(2):373-280.DOI:10.7506/spkx1002-6630-201802043.
[10]何铁光,杨丽涛,李杨瑞,等.铁皮石斛原球茎多糖DCPP1a-1对氧自由基和脂质过氧化的影响[J].天然产物研究与开发,2007(3):410-414.DOI:10.3969/j.issn.1001-6880.2007.03.012.
[11]HSIEH Y S Y.CHIEN C.LIAO S K S,et al.Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense[J].Bioorganic and Medicinal Chemistry,2008,16(11):6054-6068.DOI:10.1016/j.bmc.2008.04.042.
[12]吴月国,赵铮蓉,张萍,等.铁皮石斛复方颗粒对小鼠免疫功能影响的研究[J].中华中医药学刊,2015(8):1936-1938.DOI:10.13193/j.issn.1673-7717.2015.08.043.
[13]柳莲芳.铁皮石斛的最新研究进展[J].安徽农业科学,2012,40(11):6426-6428;6430.DOI:10.3969/j.issn.0517-6611.2012.11.029.
[14]WANG J H,LUO J P,ZHAO X Q,et al.Comparison of antitumor activities of different polysaccharide fractions from the stems Dendrobium nobile Lindl[J].Carbohydrate Polymers,2010,79(1):114-118.DOI:10.1016/j.carbpol.2009.07.032.
[15]袁慧琦,梁楚燕,梁健,等.铁皮石斛对小鼠急性酒精性肝损伤的保护作用[J].暨南大学学报(自然科学与医学版),2016,37(5):384-388.DOI:10.11778/j.jdxb.2016.05.005.
[16]聂春艳,汪鹤,潘利华,等.霍山石斛水溶性多糖抗亚急性酒精性肝损伤研究[J].安徽农业科学,2017,45(17):100-105.DOI:10.3969/j.issn.0517-6611.2017.17.035.
[17]黄静,李胜立,赵宏伟,等.霍山石斛多糖对四氯化碳致急性肝损伤小鼠的保护作用[J].中国中药杂志,2013(4):528-532.DOI:10.4268/cjcmm20130413.
[18]孙青,姜迎,高源,等.番茄红素对小鼠酒精性肝损伤的拮抗作用[J].中国食品卫生杂志,2017,29(2):145-148.DOI:10.13590/j.cjfh.2017.02.006.
[19]苗亮,刘永红,华蕾,等.葛花醇提取液对酒精中毒小鼠模型作用的研究[J].中国医药生物技术,2009(4):262-264.DOI:10.3969/cmba.j.issn.1673-713X.2009.04.005.
[20]彭勃.橄榄解酒饮对大小鼠急性酒精性肝损伤脂质代谢的影响[J].河南中医学院学报,2003,18(1):16-17.DOI:10.3969/j.issn.1674-8999.2003.01.007.
[21]DE MINICIS S,BRENNER D A.Oxidative stress in alcoholic liver disease:role of NADPH oxidase complex[J].Journal of Gastroenterology and Hepatology,2008,23(1):98-103.DOI:10.1111/j.1440-1746.2007.05277.x.
[22]BRUHA R,DVORAK K,PETRTYL J.Alcoholic liver disease[J].World Journal of Hepatology,2012,4(3):81-90.DOI:10.4254/wjh.v4.i3.81.
[23]WILLNER I R,REUBEN A.Alcohol and the liver[J].Current Opinion in Gastroenterology,2005,21(3):323-330.DOI:10.1097/01.mog.0000160044.87933.87.
[24]MANDREKAR P,SZABO G.Signalling pathways in alcohol-induced liver inflammation[J].Journal of Hepatology,2009,50(6):1258-1266.DOI:10.1016/j.jhep.2009.03.007.
[25]张玲,李俊,黄艳,等.野菊花总黄酮对酒精致急性肝损伤小鼠的保护作用[J].安徽医药,2011,15(10):1197-1199.DOI:10.3969/j.issn.1009-6469.2011.10.004.
[26]CAO Y W,JIANG Y,ZHANG D Y,et al.Protective effects of Penthorum chinense Pursh against chronic ethanol-induced liver injury in mice[J].Journal of Ethnopharmacology,2015,161:92-98.DOI:10.1016/j.jep.2014.12.013.
[27]SU L L,HUANG Y Y,FENG D S,et al.Protective effects of areca inflorescence tea on alcohol-induced hepatic injury in rats[J].Modern Food Science&Technology,2017,33(6):15-18;8.DOI:10.13982/j.mfst.1673-9078.2017.6.003.
[28]孟海涛,汪鹤,查学强,等.霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤活性的比较研究[J].食品科学,2015,36(13):229-234.DOI:10.7506/spkx1002-6630-201513042.
[29]田长城,罗建平.霍山石斛中不同多糖组分的保肝活性[J].食品科学,2015,36(7):162-166.DOI:10.7506/spkx1002-6630-201507030.
[30]CHENG D,KONG H.The effect of Lycium barbarum polysaccharide on alcohol-induced oxidative stress in rats[J].Molecules,2011,16(3):2542-2550.DOI:10.3390/molecules16032542.
[31]杨小红,袁江,周远明,等.白首乌粗多糖对酒精性肝损伤的保护作用研究[J].时珍国医国药,2009,20(11):2704-2705.DOI:10.3969/j.issn.1008-0805.2009.11.023.
[32]孙卉,苑荣爽,李贺,等.五味子、黄芪混合多糖的提取及对小鼠急性酒精性肝损伤的保护作用[J].食品科学,2017,38(2):278-282.DOI:10.7506/spkx1002-6630-201702043.
[33]童微,余强,李虎,等.铁皮石斛多糖化学修饰及其对免疫活性的影响[J].食品科学,2017,38(7):155-160.DOI:10.7506/spkx1002-6630-201707025.
[34]ZHANG G Y,NIE S P,HUANG X J,et al.Study on Dendrobium officinale O-acetyl-glucomannan(Dendronan):part VII.improving effects on colonic health of mice[J].Journal of Agricultural and Food Chemistry,2015,64(12):2485-2491.DOI:10.1021/acs.jafc.5b03117.
[2]BRANDON-WARNER E,SCHRUM L W,SCHMIDT C M,et al.Rodent models of alcoholic liver disease:of mice and men[J].Alcohol,2012,46(8):715-725.DOI:10.1016/j.alcohol.2012.08.004.
[3]CRABB D W,GALLI A,FISCHER M,et al.Molecular mechanisms of alcoholic fatty liver:role of peroxisome proliferator-activated receptor alpha[J].Alcohol,2004,34(1):35-38.DOI:10.1016/j.alcohol.2004.07.005.
[4]汤小华.铁皮石斛对肝损伤模型小鼠作用研究[D].温州:温州医学院,2010:20-25.
[5]赵爽,荣成博,张淑曼,等.毛木耳多糖对乙醇性肝损伤的保护作用[J].江苏农业科学,2017(12):142-144.DOI:10.15889/j.issn.1002-1302.2017.12.037.
[6]胡德坤,沈业寿.天麻多糖-2对小鼠四氯化碳肝损伤和酒精肝损伤的保护作用[J].中国中医药信息杂志,2007,14(12):29-31.DOI:10.3969/j.issn.1005-5304.2007.12.014.
[7]印会河,童瑶.中医基础理论[M].2版.北京:人民卫生出版社,2007:135-135.
[8]郝杰,查学强,鲍素华,等.霍山石斛不同分子量多糖体外抗氧化研究[J].食品科学,2009,30(15):94-98.DOI:10.3321/j.issn:1002-6630.2009.15.021.
[9]邱现创,赵宁,李晨,等.铁皮石斛多糖提取工艺优化及对果蝇抗氧化能力的影响[J].食品科学,2018,39(2):373-280.DOI:10.7506/spkx1002-6630-201802043.
[10]何铁光,杨丽涛,李杨瑞,等.铁皮石斛原球茎多糖DCPP1a-1对氧自由基和脂质过氧化的影响[J].天然产物研究与开发,2007(3):410-414.DOI:10.3969/j.issn.1001-6880.2007.03.012.
[11]HSIEH Y S Y.CHIEN C.LIAO S K S,et al.Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense[J].Bioorganic and Medicinal Chemistry,2008,16(11):6054-6068.DOI:10.1016/j.bmc.2008.04.042.
[12]吴月国,赵铮蓉,张萍,等.铁皮石斛复方颗粒对小鼠免疫功能影响的研究[J].中华中医药学刊,2015(8):1936-1938.DOI:10.13193/j.issn.1673-7717.2015.08.043.
[13]柳莲芳.铁皮石斛的最新研究进展[J].安徽农业科学,2012,40(11):6426-6428;6430.DOI:10.3969/j.issn.0517-6611.2012.11.029.
[14]WANG J H,LUO J P,ZHAO X Q,et al.Comparison of antitumor activities of different polysaccharide fractions from the stems Dendrobium nobile Lindl[J].Carbohydrate Polymers,2010,79(1):114-118.DOI:10.1016/j.carbpol.2009.07.032.
[15]袁慧琦,梁楚燕,梁健,等.铁皮石斛对小鼠急性酒精性肝损伤的保护作用[J].暨南大学学报(自然科学与医学版),2016,37(5):384-388.DOI:10.11778/j.jdxb.2016.05.005.
[16]聂春艳,汪鹤,潘利华,等.霍山石斛水溶性多糖抗亚急性酒精性肝损伤研究[J].安徽农业科学,2017,45(17):100-105.DOI:10.3969/j.issn.0517-6611.2017.17.035.
[17]黄静,李胜立,赵宏伟,等.霍山石斛多糖对四氯化碳致急性肝损伤小鼠的保护作用[J].中国中药杂志,2013(4):528-532.DOI:10.4268/cjcmm20130413.
[18]孙青,姜迎,高源,等.番茄红素对小鼠酒精性肝损伤的拮抗作用[J].中国食品卫生杂志,2017,29(2):145-148.DOI:10.13590/j.cjfh.2017.02.006.
[19]苗亮,刘永红,华蕾,等.葛花醇提取液对酒精中毒小鼠模型作用的研究[J].中国医药生物技术,2009(4):262-264.DOI:10.3969/cmba.j.issn.1673-713X.2009.04.005.
[20]彭勃.橄榄解酒饮对大小鼠急性酒精性肝损伤脂质代谢的影响[J].河南中医学院学报,2003,18(1):16-17.DOI:10.3969/j.issn.1674-8999.2003.01.007.
[21]DE MINICIS S,BRENNER D A.Oxidative stress in alcoholic liver disease:role of NADPH oxidase complex[J].Journal of Gastroenterology and Hepatology,2008,23(1):98-103.DOI:10.1111/j.1440-1746.2007.05277.x.
[22]BRUHA R,DVORAK K,PETRTYL J.Alcoholic liver disease[J].World Journal of Hepatology,2012,4(3):81-90.DOI:10.4254/wjh.v4.i3.81.
[23]WILLNER I R,REUBEN A.Alcohol and the liver[J].Current Opinion in Gastroenterology,2005,21(3):323-330.DOI:10.1097/01.mog.0000160044.87933.87.
[24]MANDREKAR P,SZABO G.Signalling pathways in alcohol-induced liver inflammation[J].Journal of Hepatology,2009,50(6):1258-1266.DOI:10.1016/j.jhep.2009.03.007.
[25]张玲,李俊,黄艳,等.野菊花总黄酮对酒精致急性肝损伤小鼠的保护作用[J].安徽医药,2011,15(10):1197-1199.DOI:10.3969/j.issn.1009-6469.2011.10.004.
[26]CAO Y W,JIANG Y,ZHANG D Y,et al.Protective effects of Penthorum chinense Pursh against chronic ethanol-induced liver injury in mice[J].Journal of Ethnopharmacology,2015,161:92-98.DOI:10.1016/j.jep.2014.12.013.
[27]SU L L,HUANG Y Y,FENG D S,et al.Protective effects of areca inflorescence tea on alcohol-induced hepatic injury in rats[J].Modern Food Science&Technology,2017,33(6):15-18;8.DOI:10.13982/j.mfst.1673-9078.2017.6.003.
[28]孟海涛,汪鹤,查学强,等.霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤活性的比较研究[J].食品科学,2015,36(13):229-234.DOI:10.7506/spkx1002-6630-201513042.
[29]田长城,罗建平.霍山石斛中不同多糖组分的保肝活性[J].食品科学,2015,36(7):162-166.DOI:10.7506/spkx1002-6630-201507030.
[30]CHENG D,KONG H.The effect of Lycium barbarum polysaccharide on alcohol-induced oxidative stress in rats[J].Molecules,2011,16(3):2542-2550.DOI:10.3390/molecules16032542.
[31]杨小红,袁江,周远明,等.白首乌粗多糖对酒精性肝损伤的保护作用研究[J].时珍国医国药,2009,20(11):2704-2705.DOI:10.3969/j.issn.1008-0805.2009.11.023.
[32]孙卉,苑荣爽,李贺,等.五味子、黄芪混合多糖的提取及对小鼠急性酒精性肝损伤的保护作用[J].食品科学,2017,38(2):278-282.DOI:10.7506/spkx1002-6630-201702043.
[33]童微,余强,李虎,等.铁皮石斛多糖化学修饰及其对免疫活性的影响[J].食品科学,2017,38(7):155-160.DOI:10.7506/spkx1002-6630-201707025.
[34]ZHANG G Y,NIE S P,HUANG X J,et al.Study on Dendrobium officinale O-acetyl-glucomannan(Dendronan):part VII.improving effects on colonic health of mice[J].Journal of Agricultural and Food Chemistry,2015,64(12):2485-2491.DOI:10.1021/acs.jafc.5b03117.