亚硝基胍与紫外诱变选育高效解钾菌株
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摘要
为构建遗传背景差异较大、解钾性能稳定提高的候选菌库,选用课题组分离的蜡样芽孢杆菌L18(Bacillus cereus,KF494191)为出发菌株,对其分别进行亚硝基胍(NTG)和紫外(UV)诱变处理,经四苯硼钠-季铵盐显色初筛,摇瓶发酵复筛,获得42株解钾能力显著增强的突变株,其中:诱变株L18-NTG-151和L18-UV-68发酵液钾质量浓度分别为126.74,121.69mg/L,与出发菌株相比分别提高了63.64%和57.12%.结果表明亚硝基胍和紫外线诱变处理能显著提高蜡样芽胞杆菌的解钾能力.
In order to build a bacteria library with large difference genetic background and the Potassiumsolubilizing performance,an original Silicate bacteria L18(Bacillus cereus,KF494191)was selected.It was induced mutagenesis by nitrosoguanidine(NTG)culturing and ultraviolet(UV)radiating.42 mutant bacteria with obviousincreased potassium-solubilizing performance were screened from a large number of mutant bacteria.The K content in fermentation of 2 mutant bacteria(L18-NTG-151,L18-UV-68)were126.74mg/L and 121.69 mg/L,which were improved by 63.64% and 57.12% compared with original bacteria L18(77.45mg/L).The results show thatboth two methods of NTG or UV can significantly improve the potassium-solubilizing capacity of Bacillus cereus.
In order to build a bacteria library with large difference genetic background and the Potassiumsolubilizing performance,an original Silicate bacteria L18(Bacillus cereus,KF494191)was selected.It was induced mutagenesis by nitrosoguanidine(NTG)culturing and ultraviolet(UV)radiating.42 mutant bacteria with obviousincreased potassium-solubilizing performance were screened from a large number of mutant bacteria.The K content in fermentation of 2 mutant bacteria(L18-NTG-151,L18-UV-68)were126.74mg/L and 121.69 mg/L,which were improved by 63.64% and 57.12% compared with original bacteria L18(77.45mg/L).The results show thatboth two methods of NTG or UV can significantly improve the potassium-solubilizing capacity of Bacillus cereus.
引文
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[2]商照聪,刘刚,包剑,等.我国钾资源开发技术进展与展望[J].化肥工业,2012,39(4):5-8.
[3]陈静.含钾岩石资源开发利用及前景预测[J].化工矿产地质,2000,22(1):58-63.
[4]黄珂,孟小伟,王光龙.钾长石提钾研究的进展[J].磷肥与复肥,2011,26(5):16-19.
[5]彭清静.用硫-氟混酸从钾长石中提钾的研究[J].吉首大学学报(自然科学版),1996,17(2):62-65.
[6]刘善科,韩成,刘建明,等.水热法从钾长石中提取钾、硅、铝的实验研究[J].矿物学报,2009,29(3):320-326.
[7]韩效钊,姚卫棠,胡波,等.离子交换法从钾长石提钾[J].应用化学,2003,20(4):373-375.
[8]彭清静,邹晓勇,黄诚.氯化钠熔浸钾长石提钾过程[J].过程工程学报,2002,2(2):146-150.
[9]陈定盛,石林,汪碧容,等.焙烧钾长石制硫酸钾的实验研究[J].化肥工业,2006,33(6):20-23.
[10]赵飞,盛下放,黄智,等.山东地区钾矿物分解细菌的分离及生物学特性[J].生物多样性,2008,16(6):593-600.
[11]方华舟,左雪枝.稻田固氮解磷解钾菌筛选及其复合菌剂对土壤培肥作用[J].中国土壤与肥料,2014(2):82-87.
[12]任建国,王俊丽,太子参土壤固氮菌与解钾菌的分离、筛选及鉴定[J].西南师范大学学报(自然科学版),2015,40(2):59-65.
[13]汤鹏,胡佳频,易浪波,等.钾长石矿区土壤解钾菌的分离与多样性[J].中国微生态学杂志,2015,27(2):125-129.
[14]刘文玉,史应武,王杏芹,等.亚硝基胍诱变选育低温β-半乳糖苷酶高产菌[J].生物技术通报,2008(4):185-187.
[15]孙德四,王化军,张强.胶质芽孢菌HJ07的UV与NTG诱变育种及其对铝土矿浸矿效果[J].北京科技大学学报,2013,35(10):1 268-1 278.
[16]冉广芬,马海州,孟瑞英,等.四苯硼钠-季铵盐容量法快速测钾[J].盐湖研究,2009,17(2):39-42.
[17]王娟,徐靖,宋翔宇.一株硅酸盐杆菌的紫外诱变育种[C/OL].河南省地质学会.河南地球科学通报2009年卷(下册).2009:440-443[2016-06-27].http://cpfd.cnki.com.cn/Article/CPFDTOTAL-DZXH200908002048.htm.
[18]王扬,张力,李伟星,等.亚硝基胍诱变选育L-苏氨酸高产菌的研究[J].贵州农业科学,2010,38(4):107-109.
[19]秦文旺,牧耀贵,吕利华,等.一株解钾高活性胶质芽孢杆菌的筛选与育种[J].山西农业科学,2015,43(4):434-438.
[20]盛下放,黄为一.硅酸盐细菌NBT菌株解钾机理初探[J].土壤学报,2002,39(6):863-871.
[21]易浪波,彭清忠,何齐庄,等.高效钾长石分解菌株的筛选、鉴定及解钾活性研究[J].中国微生态学杂志,2012,24(9):773-776.
[22]徐琛.产淀粉酶芽孢杆菌的筛选和分离[J].齐鲁师范学院学报,2011,26(5):144-146.
[23]林峻,施碧红,施巧琴,等.基因组改组技术快速提高扩展青霉碱性脂肪酶产量[J].生物工程学报,2007,23(4):672-676.