杭白菊黄酮对高糖诱导的血管内皮细胞Bax、Bcl-2mRNA表达的影响
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摘要
目的:研究高糖诱导下,杭白菊黄酮对人脐静脉血管内皮细胞(HUVEC)表达Bax、Bcl-2的影响。方法:将HUVEC分为空白对照组、正常血糖组、高糖刺激组和杭白菊高、中、低剂量组,作用24和48h后,观察各组细胞形态变化,采用MTT法检测细胞生长状况,RT-PCR法检测内皮细胞Bax、Bcl-2的表达。结果:高糖诱导下,杭白菊黄酮可下调Bax的表达,上调Bcl-2的表达,而杭白菊黄酮呈剂量依赖性抑制Bax表达,增强Bcl-2表达。结论:杭白菊黄酮可通过下调高糖诱导的血管内皮细胞Bax表达,上调Bcl-2表达,抑制血管内皮细胞凋亡,在一定程度上有助于防治糖尿病血管病变,为临床应用杭白菊防治糖尿病患者血管并发症提供理论参考。
Objective: To study the effects of flavonoids in Chrysanthemum morifolium on the expression of Bax and Bcl-2 in human umbilical vein endothelial cells(HUVECs) induced by high sugar. Methods: The HUVECs were divided into a blank control group, a normal blood sugar group, a high, medium, low dosage group of chrysanthemum morifolium. The cells morphologic change in each group was observed, MTT method was used to examine the growth condition of cells, RT-PCR was used to detect the expression of Bax and Bcl-2 in vascular endothelial cell after 24 and 48 hours. Results: The induction of high sugar declined the expression of Bax and raised the expression of Bcl-2. And the flavonoids in Chrysanthemum morifolium was dependently correlated with its dosage to restrain the expression of Bax and enhance the expression of Bcl-2. Conclusion: Through the down-regulation of the expression of Bax in vascular endothelial cell and the up-regulation of the expression of Bcl-2 by the induction of high sugar, the flavonoids in Chrysanthemum morifolium could restrain vascular endothelial cell. To some extent, the flavonoids in Chrysanthemum morifolium could prevent and cure the vasculopathy in diabetes, and could provides theory refference.
Objective: To study the effects of flavonoids in Chrysanthemum morifolium on the expression of Bax and Bcl-2 in human umbilical vein endothelial cells(HUVECs) induced by high sugar. Methods: The HUVECs were divided into a blank control group, a normal blood sugar group, a high, medium, low dosage group of chrysanthemum morifolium. The cells morphologic change in each group was observed, MTT method was used to examine the growth condition of cells, RT-PCR was used to detect the expression of Bax and Bcl-2 in vascular endothelial cell after 24 and 48 hours. Results: The induction of high sugar declined the expression of Bax and raised the expression of Bcl-2. And the flavonoids in Chrysanthemum morifolium was dependently correlated with its dosage to restrain the expression of Bax and enhance the expression of Bcl-2. Conclusion: Through the down-regulation of the expression of Bax in vascular endothelial cell and the up-regulation of the expression of Bcl-2 by the induction of high sugar, the flavonoids in Chrysanthemum morifolium could restrain vascular endothelial cell. To some extent, the flavonoids in Chrysanthemum morifolium could prevent and cure the vasculopathy in diabetes, and could provides theory refference.
引文
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[13]王雷,刘明.苦参碱对大肠癌细胞凋亡及Bax、Bcl-2表达的影响.中国肿瘤,2012,21(3):237-240
[14]张片红,唐沪强,郑鸣之,等.杭白菊总黄酮对衰老小鼠学习记忆的影响.中国应用生理学杂志,2011,27(3):368-371
[15]李君珂,姜子涛,李荣,等.首乌叶黄酮抗氧化性能及清除自由基能力的研究.中国食品添加剂,2012(2):69-74
[16]康文艺,黄嬛,廉婷婷,等.杭白菊保肝作用研究.天然产物研究与开发,2012,24(11):1634-1636
[17]彭钰芳,邹文韬,许传莲.杭白菊中黄酮类化合物及其抑制人源肿瘤细胞增殖活性的研究.中国药学杂志,2010,45(19):1454-1459
[18]AnsóE,Zuazo A,Irigoyen M,et al.Flavonoids inhibit hypoxiainduced vascular endothelial growth factor expression by a HIF-1 independent mechanism.Biochem Pharmacol,2010,79(11):1600-1609
[19]钟爱娇,姜哲,李雪征,等.杭白菊化学成分和药理活性的研究进展.现代药物与临床,2014,29(7):824-830
[20]陈永钧,龙晓英,潘素静,等.黄酮类化合物的药效机制及构效关系研究进展.中国实验方剂学杂志,2013,19(11):337-344
[21]彭志允,陈利国,范志勇,等.丹参酮ⅡA对血管内皮细胞损伤后v WF和TM m RNA表达的影响.中华中医药杂志,2014,29(3):708-710
[22]曹运长,文红波,吴玉兰,等.金樱子总黄酮对过氧化氢损伤内皮细胞的保护作用.时珍国医国药,2013,24(5):1159-1161
[2]Zanetti M,Stocca A,Dapas B,et al.Inhibitory effects of fenofibrate on apoptosis and cell proliferation in human endothelial cells in high glucose.J Mol Med(Berl),2007,86(2):185-195
[3]Chen G,Chen Y,Chen H,et al.The effect of NF-κB pathway on proliferation and apoptosis of human umbilical vein endothelial cells induced by intermittent high glucose.Mol Cell Biochem,2011,347(1-2):127-133
[4]Devi T S,Hosoya K,Terasaki T,et al.Critical role of TX NIP in oxidative stress,DNA damage,retinal pericyte apoptosis under high glucose:implications for diabetic retinopathy.Exp Cell Res,2013,319(7):1001-1012
[5]邱雅慧.血管内皮细胞的功能以及损伤修复与动脉粥样硬化.中国组织工程研究与临床康复,2007,11(10):1927-1929,1933
[6]Park S,Kim J A,Choi S,et al.Superoxide is a potential culprit of caspase-3 dependent endothelial cell death induced by lysophosphatidylcholine.J Physiol Pharmacol,2010,61(4):375-381
[7]Mac Eneaney O J,De Souza C A,Weil B R,et al.Prehypertension and endothelial progenitor cell function.J Hum Hypertens,2010,25(1):57-62
[8]Yoon S J,Yoon Y W,Lee B K,et al.Potential role of HMG Co A reductase inhibitor on oxidative stress induced by advanced glycation endproducts in vascular smooth muscle cells of diabetic vasculopathy.Exp Mol Med,2009,41(11):802-811
[9]Hwang H H,Moon P G,Lee J E,et al.Identification of the target proteins of rosiglitazone in 3T3-L1 adipocytes through proteomic analysis of cytosolic and secreted proteins.Mol Cells,2011,31(3):239-246
[10]Driák D,DvorskáM,SvandováI,et al.Changes in expression of some apoptotic markers in different types of human endometrium.Folia Biol(Praha),2011,57(3):104-111
[11]Westphal D,Dewson G,Czabotar P E,et al.Molecular biology of Bax and Bak activation and action.Biochim Biophys Acta,2011,1813(4):521-531
[12]赫小龙,陈铁龙,祝光礼,等.黄芪失笑散对急性心肌梗死大鼠Caspase-3、Bax及Bcl-2表达的影响.中华中医药学刊,2012,30(2):357-359
[13]王雷,刘明.苦参碱对大肠癌细胞凋亡及Bax、Bcl-2表达的影响.中国肿瘤,2012,21(3):237-240
[14]张片红,唐沪强,郑鸣之,等.杭白菊总黄酮对衰老小鼠学习记忆的影响.中国应用生理学杂志,2011,27(3):368-371
[15]李君珂,姜子涛,李荣,等.首乌叶黄酮抗氧化性能及清除自由基能力的研究.中国食品添加剂,2012(2):69-74
[16]康文艺,黄嬛,廉婷婷,等.杭白菊保肝作用研究.天然产物研究与开发,2012,24(11):1634-1636
[17]彭钰芳,邹文韬,许传莲.杭白菊中黄酮类化合物及其抑制人源肿瘤细胞增殖活性的研究.中国药学杂志,2010,45(19):1454-1459
[18]AnsóE,Zuazo A,Irigoyen M,et al.Flavonoids inhibit hypoxiainduced vascular endothelial growth factor expression by a HIF-1 independent mechanism.Biochem Pharmacol,2010,79(11):1600-1609
[19]钟爱娇,姜哲,李雪征,等.杭白菊化学成分和药理活性的研究进展.现代药物与临床,2014,29(7):824-830
[20]陈永钧,龙晓英,潘素静,等.黄酮类化合物的药效机制及构效关系研究进展.中国实验方剂学杂志,2013,19(11):337-344
[21]彭志允,陈利国,范志勇,等.丹参酮ⅡA对血管内皮细胞损伤后v WF和TM m RNA表达的影响.中华中医药杂志,2014,29(3):708-710
[22]曹运长,文红波,吴玉兰,等.金樱子总黄酮对过氧化氢损伤内皮细胞的保护作用.时珍国医国药,2013,24(5):1159-1161