灰毡毛忍冬不同发育期花蕾中3种成分含量的变化研究
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摘要
目的比较灰毡毛忍冬不同发育期花蕾中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。方法色谱柱:CAPCELL PAK C18 MG(250 mm×4.60 mm,5μm);流动相:乙腈(A)-0.4%醋酸溶液(B),梯度洗脱;流速:1.0 mL·min~(-1);紫外检测波长:330 nm;蒸发光散射检测器条件:漂移管温度110℃,气体流速3.0 L·min~(-1),撞击器关;柱温:30℃。收集不同发育期(幼蕾期、三青期、大白期、银花期、金花期、凋花期)花蕾样品,采用HPLC-UV-ELSD串联技术,以外标两点法计算绿原酸的含量,以外标两点法对数方程计算灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。结果灰毡毛忍冬中绿原酸和川续断皂苷乙含量以三青期最高,分别为6.934%,1.800%;灰毡毛忍冬皂苷乙以幼蕾期最高,为9.351%,较三青期高0.227%。结论通过对灰毡毛忍冬花蕾不同发育期的含量比较可见,最佳采收期为三青期,此时绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙含量高。
OBJECTIVE To compare the content differences of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages of Lonicera macranthoides Hand.-Mazz. METHODS The chromatographic column was CAPCELL PAK C18 MG(250 mm×4.60 mm, 5 μm); the moblie phase was acetonitrile(A)-0.4% acetic acid(B) with gradient elution; flow rate was 1.0 mL·min~(-1); UV detection wavelength was 330 nm; ELSD testing condition was as follows: tube temperature 110 ℃, gas flow 3.0 L·min~(-1) and impactor off; column temperature was 30 ℃. Using HPLC-UV-ELSD series technology, the contents of three components in floral buds collected at different developmental stages(Youlei stage, Sanqing stage, Dabai stage, Yinhua stage, Jinhua stage, Diaohua stage) in Lonicera macranthoides Hand.-Mazz were measured. The content of chlorogenic acid was calculated by outside the two point method, and the content of macranthoidin B and dipsacoside B was calculated by outside the two point logarithmic equation method. RESULTS The contents of chlorogenic acid and dipsacoside B were 6.934% and 1.800%, respectively, were the highest at Sanqing stage in Lonicera macranthoides Hand.-Mazz.. However, the highest content of macranthoidin B was 9.351% at Youlei stage, which was 0.227% higher than that at Sanqing stage. CONCLUSION By comparing the contents of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages in Lonicera macranthoides Hand.-Mazz., Sanqing period is the best harvest time with high content.
OBJECTIVE To compare the content differences of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages of Lonicera macranthoides Hand.-Mazz. METHODS The chromatographic column was CAPCELL PAK C18 MG(250 mm×4.60 mm, 5 μm); the moblie phase was acetonitrile(A)-0.4% acetic acid(B) with gradient elution; flow rate was 1.0 mL·min~(-1); UV detection wavelength was 330 nm; ELSD testing condition was as follows: tube temperature 110 ℃, gas flow 3.0 L·min~(-1) and impactor off; column temperature was 30 ℃. Using HPLC-UV-ELSD series technology, the contents of three components in floral buds collected at different developmental stages(Youlei stage, Sanqing stage, Dabai stage, Yinhua stage, Jinhua stage, Diaohua stage) in Lonicera macranthoides Hand.-Mazz were measured. The content of chlorogenic acid was calculated by outside the two point method, and the content of macranthoidin B and dipsacoside B was calculated by outside the two point logarithmic equation method. RESULTS The contents of chlorogenic acid and dipsacoside B were 6.934% and 1.800%, respectively, were the highest at Sanqing stage in Lonicera macranthoides Hand.-Mazz.. However, the highest content of macranthoidin B was 9.351% at Youlei stage, which was 0.227% higher than that at Sanqing stage. CONCLUSION By comparing the contents of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages in Lonicera macranthoides Hand.-Mazz., Sanqing period is the best harvest time with high content.
引文
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[2]KANG S,ZHANG J,WANG Y D,et al.Pharmacognostic authentication study for Lonicerae Japonicae Flos and Lonicerae Flos[J].Chin J Pharm Anal(药物分析杂志),2014,34(11):1913-1921.
[3]GAO L x.Determination of total tritorpenoid saponin in different buds stages of lonicerae flos[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(10):1110-1113.
[4]李锦燊,吴洪文.山银花化学成分与药理活性研究进展[J].北方药学,2014,11(2):71-73.
[5]CHEN J,XU X F,CHAI X Y,et al.Chemical constituents in the buds of Lonicera macranthoides[J].Chin J Nat Med(中国天然药物),2006,4(5):347-351.
[6]HU Y F,WU C C.Chemical constituents from stems of Lonicera macranthoides[J].J Chin Med Mater(中药材),2012,35(1):66-69.
[7]YANG X X,BAO Y R,WANG S,et al.Study on the quality evaluation of honeysuckle in different harvest time[J].Lishizhen Med(44)ater Med Res(时珍国医国药),2015,26(6):1466-1468.
[8]GAO L X.Determination of total tritorpenoid saponin in different buds stages of Lonicerae Flos[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(10):1110-1113.
[9]GUO G Y,LIU L,HU Q,et al.Compare study on the content of chemical components and quality of different period's spring xinyangmaojian tea[J].Food Sci Technol(食品科技),2007(9):141-144.
[10]XU X Y,ZHENG Y M,FU S Q,et al.Determination of content of capsaicin in Capsicum annuum by HPLC[J].Sci Technol Food Ind(食品工业科技),2007,28(11):229-233.
[11]容穗华,林海,高妮.不同产地当归中主要有效成分的含量测定[J].当代医学,2011,17(22):140-141.