凹凸棒石/羟基磷灰石/聚己内酯/胶原构建的骨修复材料
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摘要
背景:骨移植是修复骨缺损的惟一方法,但传统的骨移植方法均存在一定的弊端。骨组织工程可达到修复或重建骨的目的,为骨缺损患者的治疗带来新的选择。目的:构建一种新型有效提高骨缺损修复的组织工程支架材料。方法:采用化学沉淀辅以微波辐射法制备出不同Ca/P比(1.50/1.67)的羟基磷灰石,采用溶液灌注-溶剂挥发法、离子滤沥法等制备凹凸棒石/羟基磷灰石/聚己内酯/胶原、凹凸棒石/缺钙羟基磷灰石/聚己内酯/胶原、凹凸棒石/聚己内酯/胶原(对照组)复合材料。利用扫描电子显微镜、红外光谱仪、表面接触测定仪、万能力学机等,分别进行材料表征、有效成分、亲水性和力学性能评价,用动物实验评价材料与宿主的组织相容性。结果与结论:1精确控制p H值范围能合成不同Ca/P比的羟基磷灰石;2与凹凸棒石/聚己内酯/胶原复合材料相比,构建的凹凸棒石/羟基磷灰石/聚己内酯/胶原和凹凸棒石/缺钙羟基磷灰石/聚己内酯/胶原复合材料的力学性能、透气性、亲水性明显增强(P<0.05),孔隙率、吸水膨胀率明显降低(P<0.05);3动物实验结果显示,支架材料与宿主无免疫炎性反应,具有良好的组织相容性;4结果提示,构建的凹凸棒石/羟基磷灰石(1.50,1.67)/聚己内酯/胶原复合支架材料有可能成为较为理想的骨组织修复替代支架材料。
BACKGROUND: Bone transplantation is the only method for the repair of bone defects. However, traditional bone transplantation has some disadvantages. Bone tissue engineering, as a new treatment strategy, can achieve the desire therapeutic outcomes.OBJECTIVE: To fabricate a new tissue-engineered scaffold for improving bone repair effectively. METHODS: Hydroxyapatites(HA) with different Ca/P(1.50/1.67) ratios were synthesized by chemical precipitation method and microwave radiation method. Composite scaffolds of palygorskite(APC)/HA/polycaprolactone(PCL)/ collagen(COL), APC/calcium deficiency HA(CDHA)/PCL/COL, and APC/PCL/COL(control group) were prepared by solution perfusion-solvent evaporation and ion leaching method. The material characterization, active ingredients, hydrophilic property, and mechanical properties were evaluated by scanning electron microscope, infrared spectrometer, surface contact measuring instrument and universal mechanics, respectively. The histocompatibility of the implant with the host was assessed through animal experiments. RESULTS AND CONCLUSION: By precise control of p H range, HA with different Ca/P ratios could be synthesized. The mechanical properties, air permeability, hydrophilic property of the APC/HA/PCL/COL and APC/CDHA/PCL/COL composite materials were significantly increased compared with the APC/PCL/COL composite material(P < 0.05), while the porosity, water absorption expansion rate were significantly decreased(P < 0.05). Results from our animal experiments showed that no immune inflammatory reaction was observed suggesting that the composite materials hold good histocompatibility. To conclude, the APC/HA(1.50/1.67)/PCL/COL composite materials are promising bone substitutes in bone tissue repair.
BACKGROUND: Bone transplantation is the only method for the repair of bone defects. However, traditional bone transplantation has some disadvantages. Bone tissue engineering, as a new treatment strategy, can achieve the desire therapeutic outcomes.OBJECTIVE: To fabricate a new tissue-engineered scaffold for improving bone repair effectively. METHODS: Hydroxyapatites(HA) with different Ca/P(1.50/1.67) ratios were synthesized by chemical precipitation method and microwave radiation method. Composite scaffolds of palygorskite(APC)/HA/polycaprolactone(PCL)/ collagen(COL), APC/calcium deficiency HA(CDHA)/PCL/COL, and APC/PCL/COL(control group) were prepared by solution perfusion-solvent evaporation and ion leaching method. The material characterization, active ingredients, hydrophilic property, and mechanical properties were evaluated by scanning electron microscope, infrared spectrometer, surface contact measuring instrument and universal mechanics, respectively. The histocompatibility of the implant with the host was assessed through animal experiments. RESULTS AND CONCLUSION: By precise control of p H range, HA with different Ca/P ratios could be synthesized. The mechanical properties, air permeability, hydrophilic property of the APC/HA/PCL/COL and APC/CDHA/PCL/COL composite materials were significantly increased compared with the APC/PCL/COL composite material(P < 0.05), while the porosity, water absorption expansion rate were significantly decreased(P < 0.05). Results from our animal experiments showed that no immune inflammatory reaction was observed suggesting that the composite materials hold good histocompatibility. To conclude, the APC/HA(1.50/1.67)/PCL/COL composite materials are promising bone substitutes in bone tissue repair.
引文
[1]Neufurth M,Wang X,Schr?der HC,et al.Engineering a morphogenetically active hydrogel for bioprinting of bioartificial tissuederived from humanosteoblast-like Sa OS-2cells.Biomaterials.2014;35(31):8810-8819.
[2]Hashemibeni B,Dehghani L,Sadeghi F,et al.Bone Repair with Differentiated Osteoblasts from Adipose-derived Stem Cells in Hydroxyapatite/Tricalcium Phosphate In vivo.Int J Prev Med.2016;7:62.
[3]徐世希,欧阳丽娜,丁劲松.凹凸棒石黏土及其应用进展[J].中南药学,2009,7(6):441-445.
[4]王浩,张里程,石涛,等.胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白骨修复材料的性质评估[J].北京大学学报(医学版),2011,43(5):730-734.
[5]Castilho M,Moseke C,Ewald A,et al.Direct 3D powder printing of biphasic calcium phosphate scaffolds for substitution of complex bone defects.Biomaterial.2014;6(1):015006.
[6]Xue J,He M,Liu H,et al.Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes.Biomaterials.2014;35(34):9395-9405.
[7]艾合麦提,玉素甫,陈统一,等.可降解聚己内酯修复骨缺损的实验研究[J].中国修复重建外科杂志,2005,19(6):439-442.
[8]张达江,王亮.Ⅰ型胶原蛋白的结构、功能及其应用研究的现状与前景[J].生物技术通讯,2006,17(2):265-269.
[9]何立志,冯祖德.不同Ca/P纳米缺钙羟基磷灰石的微波合成和表征[J].广州化工,2009,37(4):74-76.
[10]王学江,李玉宝.羟基磷灰石纳米针晶与聚酰胺仿生复合生物材料研究[J].高技术通讯,2001,11(5):1-5.
[11]王迎军,杜昶,赵娜如,等.仿生人工骨修复材料研究[J].华南理工大学学报,2012,40(10):51-58.
[12]李强.可注射骨组织工程材料的制备与性能研究[D].华中科技大学,2009.
[13]帅丽.缺钙纳米羟基磷灰石/胶原复合支架材料的制备[D].重庆大学,2010
[14]吴晓东.生物活性骨组织工程支架的研究及介孔羟基磷灰石的初步探讨[D].吉林大学,2012.
[15]朱爱臣.一种可用于引导组织再生技术的淀粉/PVA膜的制备及性能研究[D].北京化工大学,2010.
[16]Park HJ,Yu SJ,Yang K,et al.Paper-based bioactive scaffolds for stem-mediated bone tissue engineering.Biomaterial.2014;35(37):9811-9823.
[17]Li H,Xue K,Kong N,et al.Silicate bioceramics enhanced vascularization and osteogensis through stimulating in teractions between endothelia cells and bone marrow stromal cells.Biomaterials.2014;35(12):3803-3818.
[18]Hettiaratchi MH,Miller T,Temenoff JS,et al.Heparin microparticle effects on presentation and bioactivity of bone morphogenetic protein-2.Biomaterials.2014;35(25):7228-7238.
[19]Cholas R,Kunjalukkal Padmanabhan S,et al.Scaffolds for bone regeneration made of hydroxyapatite microspheres in a collagen matrix.Mater Sci Eng C Mater Biol Appl.2016;63:499-505.
[20]Castilho M,Moseke C,Ewald A,et al.Direct 3D powder printing of biphasic calcium phosphate scaffolds for substitution of complex bone defects.Biofabrication.2014;6(1):015006.
[21]Xue J,He M,Liu H,et al.Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes.Biomaterials.2014;35(34):9395-9405.
[22]贾帅军.定向结构软骨支架复合骨髓基质干细胞修复兔关节软骨缺损的研究[D].第四军医大学,2012.
[23]Han B,Perelman N,Tang B,et al.Collagen-targeted BMP3 fusion proteins arrayed on collagen matrices or porous ceramics impregnated with Type I collagen enhance osteogenesis in a rat cranial defect model.Orthop Res.2002;20(4):747-755.
[24]Kume S,Kato S,Yamagishi S,et al.Advanced glycation end-products attenuate human mesenchymal stem cells and prevent cognate differentiation into adipose tissue,cartilage,and bone.Bone Miner Res.2005;20(9):1647-1658.
[2]Hashemibeni B,Dehghani L,Sadeghi F,et al.Bone Repair with Differentiated Osteoblasts from Adipose-derived Stem Cells in Hydroxyapatite/Tricalcium Phosphate In vivo.Int J Prev Med.2016;7:62.
[3]徐世希,欧阳丽娜,丁劲松.凹凸棒石黏土及其应用进展[J].中南药学,2009,7(6):441-445.
[4]王浩,张里程,石涛,等.胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白骨修复材料的性质评估[J].北京大学学报(医学版),2011,43(5):730-734.
[5]Castilho M,Moseke C,Ewald A,et al.Direct 3D powder printing of biphasic calcium phosphate scaffolds for substitution of complex bone defects.Biomaterial.2014;6(1):015006.
[6]Xue J,He M,Liu H,et al.Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes.Biomaterials.2014;35(34):9395-9405.
[7]艾合麦提,玉素甫,陈统一,等.可降解聚己内酯修复骨缺损的实验研究[J].中国修复重建外科杂志,2005,19(6):439-442.
[8]张达江,王亮.Ⅰ型胶原蛋白的结构、功能及其应用研究的现状与前景[J].生物技术通讯,2006,17(2):265-269.
[9]何立志,冯祖德.不同Ca/P纳米缺钙羟基磷灰石的微波合成和表征[J].广州化工,2009,37(4):74-76.
[10]王学江,李玉宝.羟基磷灰石纳米针晶与聚酰胺仿生复合生物材料研究[J].高技术通讯,2001,11(5):1-5.
[11]王迎军,杜昶,赵娜如,等.仿生人工骨修复材料研究[J].华南理工大学学报,2012,40(10):51-58.
[12]李强.可注射骨组织工程材料的制备与性能研究[D].华中科技大学,2009.
[13]帅丽.缺钙纳米羟基磷灰石/胶原复合支架材料的制备[D].重庆大学,2010
[14]吴晓东.生物活性骨组织工程支架的研究及介孔羟基磷灰石的初步探讨[D].吉林大学,2012.
[15]朱爱臣.一种可用于引导组织再生技术的淀粉/PVA膜的制备及性能研究[D].北京化工大学,2010.
[16]Park HJ,Yu SJ,Yang K,et al.Paper-based bioactive scaffolds for stem-mediated bone tissue engineering.Biomaterial.2014;35(37):9811-9823.
[17]Li H,Xue K,Kong N,et al.Silicate bioceramics enhanced vascularization and osteogensis through stimulating in teractions between endothelia cells and bone marrow stromal cells.Biomaterials.2014;35(12):3803-3818.
[18]Hettiaratchi MH,Miller T,Temenoff JS,et al.Heparin microparticle effects on presentation and bioactivity of bone morphogenetic protein-2.Biomaterials.2014;35(25):7228-7238.
[19]Cholas R,Kunjalukkal Padmanabhan S,et al.Scaffolds for bone regeneration made of hydroxyapatite microspheres in a collagen matrix.Mater Sci Eng C Mater Biol Appl.2016;63:499-505.
[20]Castilho M,Moseke C,Ewald A,et al.Direct 3D powder printing of biphasic calcium phosphate scaffolds for substitution of complex bone defects.Biofabrication.2014;6(1):015006.
[21]Xue J,He M,Liu H,et al.Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes.Biomaterials.2014;35(34):9395-9405.
[22]贾帅军.定向结构软骨支架复合骨髓基质干细胞修复兔关节软骨缺损的研究[D].第四军医大学,2012.
[23]Han B,Perelman N,Tang B,et al.Collagen-targeted BMP3 fusion proteins arrayed on collagen matrices or porous ceramics impregnated with Type I collagen enhance osteogenesis in a rat cranial defect model.Orthop Res.2002;20(4):747-755.
[24]Kume S,Kato S,Yamagishi S,et al.Advanced glycation end-products attenuate human mesenchymal stem cells and prevent cognate differentiation into adipose tissue,cartilage,and bone.Bone Miner Res.2005;20(9):1647-1658.