UPLC-MS/MS同时测定人血浆中奥卡西平及其代谢产物的浓度
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摘要
目的建立UPLC-MS/MS色谱法测定人血浆中奥卡西平及其活性代谢产物利卡西平浓度的方法。方法色谱柱为Zorbax Eclipse Plus-C_(18)色谱柱,流动相为乙腈-1 mmol·L~(-1)甲酸铵(80/20,V/V),流速为0.2μg·mL~(-1),电喷雾离子化正离子MRM检测(ESI~+)。奥卡西平、利卡西平检测离子对分别为[M-H]~+m/z 252.90→207.95、m/z 255.00→194.00。结果奥卡西平、利卡西平血药浓度在0.6～30.0μg·mL~(-1)、0.7～70.0μg·mL~(-1)内呈良好的线性关系;方法回收率为75.2%～83.2%、71.2%～80.4%,日内、日间精密度RSD均小于15%。结论该方法简便、快速、灵敏、准确,适用于奥卡西平及其代谢产物血药浓度监测及药物动力学研究。
Objective To establish a method for the determination the concentration of oxcarbazepine and its active metabolite ricarbazepine in human plasma by UPLC-MS/MS chromatography. Methods The chromatographic column was Zorbax Eclipse Plus-C_(18), and the mobile phase was acetonitrile-1 mmol·L~(-1) ammonium formate(80/20, V/V), the flow rate was 0.2 mL·min~(-1). The sample was determined by positive electrospray ionization source MRM-ESI~+. The detection ions pair of OXC and MHD were [M-H]~+m/z 252.90→207.95 and m/z 255.00→194.00. Results A good linear relationship of OXC and MHD was in the range of 0.6-30.0 μg·mL~(-1), 0.7-70.0 μg·mL~(-1). The method recovery of OXC and MHD were75.2%-83.2%, 71.2%-80.4%. RSD of intra-day and inter-day precision were both less than 15%. Conclusion The method is simple, rapid, sensitive and acourate. It is suitable for clinical therapeutic drug monitoring and pharmacokinetic research.
Objective To establish a method for the determination the concentration of oxcarbazepine and its active metabolite ricarbazepine in human plasma by UPLC-MS/MS chromatography. Methods The chromatographic column was Zorbax Eclipse Plus-C_(18), and the mobile phase was acetonitrile-1 mmol·L~(-1) ammonium formate(80/20, V/V), the flow rate was 0.2 mL·min~(-1). The sample was determined by positive electrospray ionization source MRM-ESI~+. The detection ions pair of OXC and MHD were [M-H]~+m/z 252.90→207.95 and m/z 255.00→194.00. Results A good linear relationship of OXC and MHD was in the range of 0.6-30.0 μg·mL~(-1), 0.7-70.0 μg·mL~(-1). The method recovery of OXC and MHD were75.2%-83.2%, 71.2%-80.4%. RSD of intra-day and inter-day precision were both less than 15%. Conclusion The method is simple, rapid, sensitive and acourate. It is suitable for clinical therapeutic drug monitoring and pharmacokinetic research.
引文
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[8]张国阳,何银舟,王利.反相高效液相色谱法测定利卡西平血药浓度[J].中国药物应用与监测,2017,14(5):267-270.
[2]林奕沨,黄成坷,王哲,等.HPLC法同时检测人血浆中奥卡西平及其代谢产物浓度[J].中国临床药学杂志,2017,22(2):115-118.
[3]谢朋飞,崔宏娣,徐世希,等.RP-HPLC法同时测定人血浆中奥卡西平及利卡西平浓度[J].解放军药学学报,2017,33(4):326-329.
[4]徐善森,张媞,周敬凯,等.高效液相色谱法测定人血浆中拉莫三嗪、奥卡西平及其代谢物浓度[J].中国临床药理学杂志,2015,31(12):1176-1179.
[5]迟丹怡,李中东,焦正,等.人血浆中奥卡西平及其代谢物10-羟基卡马西平的HPLC法测定[J].药物分析杂志,2006,26(9):1195-1198.
[6]FRANCESCHI L,FURLANUT M.A simple method to monitor plasma concentrations of oxcarbazepine,carbamazepine,their main metabolites and lamotrigine in epileptic patients[J].Pharmacol Res,2005,51(4):297-302.
[7]张全英,徐文俊,俞蕴莉.高效液相色谱法测定癫痫患者血浆中奥卡西平活性代谢物的浓度及其血药浓度与疗效关系评价[J].中国生化药物杂志,2015,35(1):106-109.
[8]张国阳,何银舟,王利.反相高效液相色谱法测定利卡西平血药浓度[J].中国药物应用与监测,2017,14(5):267-270.