6种抗肿瘤中药注射液对卵巢癌细胞中ATF3 mRNA表达的影响
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摘要
目的考察消癌平注射液、艾迪注射液、华蟾素注射液、复方苦参注射液、斑蝥酸钠维生素B_6注射液、参芪扶正注射液对卵巢癌细胞A2780、SK-OV-3中转录激活因子3(ATF3)mRNA表达的影响。方法 6种不同浓度中药注射液作用于卵巢癌细胞24 h后,MTT法、RT-PCR法分别检测细胞增殖率、ATF3 mRNA表达。结果各中药注射液均有抑制卵巢癌细胞增殖的作用,并呈浓度依赖性。其中,华蟾素注射液可在抑制细胞增殖的同时抑制ATF3 mRNA表达,斑蝥酸钠维生素B_6注射液显著上调该因子mRNA表达,一定浓度消癌平注射液、艾迪注射液、复方苦参注射液、参芪扶正注射液对其mRNA表达也呈下调作用。结论 6种抗肿瘤中药注射液对卵巢癌细胞中ATF3 mRNA表达有抑制或诱导作用,其机制可能与中药活性成分影响肿瘤发展和转移有关。
引文
[1] Siegel R L, Miller K D, Jemal A. Cancer statistics, 2018[J]. CA Cancer J Clin, 2018, 68(1): 7-30.
[2] 傅柏涵, 郭放, 韩涛, 等. 消癌平注射液联合化疗治疗转移性三阴型乳腺癌临床疗效观察[J]. 解放军医药杂志, 2016, 28(6): 23-26.
[3] 李丽娜, 南克俊, 张茜. 复方斑蝥注射液联合奥沙利铂对肝癌HepG2细胞增殖与凋亡的影响[J]. 现代肿瘤医学, 2011, 19(3): 438-440.
[4] 梁倩影, 彭大为. 复方苦参注射液联合紫杉醇与顺铂治疗晚期卵巢癌的临床疗效[J]. 实用肿瘤杂志, 2015, 30(5): 465-468.
[5] 冯烨, 李占东, 孙红. 中药注射剂在抗肿瘤治疗中的应用[J]. 北方药学, 2014, 11(5): 71-72.
[6] Hasim M S, Nessim C, Villeneuve P J, et al. Activating transcription factor 3 as a novel regulator of chemotherapy response in breast cancer[J]. Translat Oncol, 2018, 11(4): 988-998.
[7] Yin X, Wolford C C, Chang Y S, et al. ATF3, an adaptive-response gene, enhances TGFβ signaling and cancer-initiating cell features in breast cancer cells[J]. J Cell Sci, 2010, 123(Pt 20): 3558-3565.
[8] Fan W, Sun L, Zhou J Q, et al. Marsdenia tenacissima extract induces G0/G1 cell cycle arrest in human esophageal carcinoma cells by inhibiting mitogen-activated protein kinase (MAPK) signaling pathway[J]. Chin J Nat Med, 2015, 13(6): 428-437.
[9] 王成双, 佐满珍. 华蟾素联合顺铂对卵巢癌细胞A2780增殖及凋亡的影响[J]. 肿瘤学杂志, 2017, 23(1): 30-34.
[10] 张岩岩, 康健. 中药艾迪诱导人喉癌细胞Hep-2凋亡的研究[J]. 军医进修学院学报, 2012, 33(1): 78-81.
[11] 吴晓慧, 白喜丰, 施喆, 等. 斑蝥酸钠维生素B6对肝癌细胞侵袭力的抑制作用及机制[J]. 中成药, 2016, 38(11): 2487-2489.
[12] 赵勇, 傅羽, 吴耀华, 等. 参芪扶正注射液与赫塞汀联合应用对乳腺癌细胞增殖和凋亡的影响[J]. 哈尔滨医科大学学报, 2013, 47(3): 232-235.
[13] Bottone F G Jr, Moon Y, Alston-Mills B, et al. Transcriptional regulation of activating transcription factor 3 involves the early growth response-1 gene[J]. J Pharmacol Exp Ther, 2005, 315(2): 668-677.
[14] 周婧倩, 钦松, 马亮亮, 等. 消癌平注射液及其三大类组分抗肿瘤细胞的迁移能力[J]. 中成药, 2015, 37(2): 256-260.
[15] Huang X, Li X, Guo B. KLF6 induces apoptosis in prostate cancer cells through up-regulation of ATF3[J]. J Biol Chem, 2008, 283(44): 29795-29801.
[16] Chang Y S, Jalgaonkar S P, Middleton J D, et al. Stress-inducible gene Atf3 in the noncancer host cells contributes to chemotherapy-exacerbated breast cancer metastasis[J]. Proc Natl Acad Sci USA, 2017, 114(34): E7159-E7168.
[17] Zhao H, Ding Y, Tie B, et al. miRNA expression pattern associated with prognosis in elderly patients with advanced OPSC and OCC[J]. Int J Oncol, 2013, 43(3): 839-849.
[18] Nguyen H T, Jia W, Beedle A M, et al. Lysophosphatidic acid mediates activating transcription factor 3 expression which is a target for post-transcriptional silencing by miR-30c-2-3p[J]. PLoS One, 2015, 10(9): e0139489.
[19] Wang Y, Li L, Qu Z, et al. The expression of miR-30a* and miR-30e* is associated with a dualistic model for grading ovarian papillary serious carcinoma[J]. Int J Oncol, 2014, 44(6): 1904-1914.
[2] 傅柏涵, 郭放, 韩涛, 等. 消癌平注射液联合化疗治疗转移性三阴型乳腺癌临床疗效观察[J]. 解放军医药杂志, 2016, 28(6): 23-26.
[3] 李丽娜, 南克俊, 张茜. 复方斑蝥注射液联合奥沙利铂对肝癌HepG2细胞增殖与凋亡的影响[J]. 现代肿瘤医学, 2011, 19(3): 438-440.
[4] 梁倩影, 彭大为. 复方苦参注射液联合紫杉醇与顺铂治疗晚期卵巢癌的临床疗效[J]. 实用肿瘤杂志, 2015, 30(5): 465-468.
[5] 冯烨, 李占东, 孙红. 中药注射剂在抗肿瘤治疗中的应用[J]. 北方药学, 2014, 11(5): 71-72.
[6] Hasim M S, Nessim C, Villeneuve P J, et al. Activating transcription factor 3 as a novel regulator of chemotherapy response in breast cancer[J]. Translat Oncol, 2018, 11(4): 988-998.
[7] Yin X, Wolford C C, Chang Y S, et al. ATF3, an adaptive-response gene, enhances TGFβ signaling and cancer-initiating cell features in breast cancer cells[J]. J Cell Sci, 2010, 123(Pt 20): 3558-3565.
[8] Fan W, Sun L, Zhou J Q, et al. Marsdenia tenacissima extract induces G0/G1 cell cycle arrest in human esophageal carcinoma cells by inhibiting mitogen-activated protein kinase (MAPK) signaling pathway[J]. Chin J Nat Med, 2015, 13(6): 428-437.
[9] 王成双, 佐满珍. 华蟾素联合顺铂对卵巢癌细胞A2780增殖及凋亡的影响[J]. 肿瘤学杂志, 2017, 23(1): 30-34.
[10] 张岩岩, 康健. 中药艾迪诱导人喉癌细胞Hep-2凋亡的研究[J]. 军医进修学院学报, 2012, 33(1): 78-81.
[11] 吴晓慧, 白喜丰, 施喆, 等. 斑蝥酸钠维生素B6对肝癌细胞侵袭力的抑制作用及机制[J]. 中成药, 2016, 38(11): 2487-2489.
[12] 赵勇, 傅羽, 吴耀华, 等. 参芪扶正注射液与赫塞汀联合应用对乳腺癌细胞增殖和凋亡的影响[J]. 哈尔滨医科大学学报, 2013, 47(3): 232-235.
[13] Bottone F G Jr, Moon Y, Alston-Mills B, et al. Transcriptional regulation of activating transcription factor 3 involves the early growth response-1 gene[J]. J Pharmacol Exp Ther, 2005, 315(2): 668-677.
[14] 周婧倩, 钦松, 马亮亮, 等. 消癌平注射液及其三大类组分抗肿瘤细胞的迁移能力[J]. 中成药, 2015, 37(2): 256-260.
[15] Huang X, Li X, Guo B. KLF6 induces apoptosis in prostate cancer cells through up-regulation of ATF3[J]. J Biol Chem, 2008, 283(44): 29795-29801.
[16] Chang Y S, Jalgaonkar S P, Middleton J D, et al. Stress-inducible gene Atf3 in the noncancer host cells contributes to chemotherapy-exacerbated breast cancer metastasis[J]. Proc Natl Acad Sci USA, 2017, 114(34): E7159-E7168.
[17] Zhao H, Ding Y, Tie B, et al. miRNA expression pattern associated with prognosis in elderly patients with advanced OPSC and OCC[J]. Int J Oncol, 2013, 43(3): 839-849.
[18] Nguyen H T, Jia W, Beedle A M, et al. Lysophosphatidic acid mediates activating transcription factor 3 expression which is a target for post-transcriptional silencing by miR-30c-2-3p[J]. PLoS One, 2015, 10(9): e0139489.
[19] Wang Y, Li L, Qu Z, et al. The expression of miR-30a* and miR-30e* is associated with a dualistic model for grading ovarian papillary serious carcinoma[J]. Int J Oncol, 2014, 44(6): 1904-1914.