摘要
同位素稀释质谱法避免了基质效应和分析条件的影响,在定量分析和准确度方面具有特殊优势,在生物分子定量分析领域得到了普遍应用。稳定同位素标记肽段作为重要内标试剂广泛应用于蛋白质组学定量及食品过敏原检测。同位素稀释质谱技术的发展将会进一步推动稳定同位素标签技术在新药研发、临床病理、蛋白质组学、食品安全等领域的研究应用。本文从生物合成法和化学合成法方面综述了稳定同位素标记多肽的合成,并分别对细胞代谢标记法、高等真核生物标记法、酶催化标记法、乙酰化标记、标记氨基酸缩合法等合成方法进行了评述;同时介绍了稳定同位素标记多肽在乳制品检测、生物蛋白定量、蛋白测序及药物代谢、食品过敏原检测中的应用。
The isotope dilution mass spectrometry can avoid the matrix effect and the influence of analysis conditions,which has quantitative analysis and the accuracy of results in absolute precision special advantage,so it is widely applied in the field of quantitative analysis of biomolecules.The stable isotopic labeled peptides are widely used as an important internal standard reagent in the quantitative of proteomicsand food allergenic detection.The development of isotopic dilution mass spectrometry will further promote the research and application of stable isotope labeling technology in new drug development,clinical pathology,proteomics and food safety.With the rapid development of stable isotope labeling technology in recent years,the synthesis of stable isotope labeling peptides was summarized briefly from two aspects of biosynthesis method and chemical method.Methods of marker of cell metabolism,higher eukaryotes tag,enzyme catalytic notation,acetylation,labeled amino acid condensation were discussedin details.The application of stable isotopic marker polypeptide in the detection of dairy products,quantitative protein,protein sequencing and drug metabolism and food allergens were introduced.
引文
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