烟叶多酚含量与相关基因表达间的关系分析
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摘要
为研究烟草多酚含量与编码苯丙氨酸解氨酶(PAL)及肉桂酸-4-羟化酶(C4H)基因表达量之间的关系,分析烤烟旺长期、现蕾期及成熟期烟叶中的多酚含量,测定PAL、C4H基因的表达量,并分析两者之间的相关关系。结果表明,在生长过程中,烟叶中的多酚含量逐渐增加,到成熟期达到最高,烤后烟叶中的多酚含量与PAL及C4H的表达量密切相关,其中与C4H的表达量呈极显著正相关。
引文
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[21]Williamson R E,Gwynn G R.Variation of polyphenols in fluecured tobacco cultivars attributed to location,stalk position,and year[J].Crop Science,1982,22(1):144-146.
[2]Louis R,Dietrich A,Hahlbrock K,et al.A phenylalanine ammonialyase gene from parsley:structure,regulation and identification of elicitor and light responsive cis-acting elements[J].EMBO Journal,1989,8(6):1641-1648.
[3]杨慧芹,王莎莎,张建波,等.烟草多酚代谢对干旱和低温胁迫的响应差异及其比较[J].基因组学与应用生物学,2015,34(3):645-654.
[4]Pellegrini L,Rohfritsch O,Fritig B,et al.Phenylalanine ammonialyase in tobacco.Molecular cloning and gene expression during the hypersensitive reaction to tobacco mosaic virus and the response to a fungal elicitor[J].Plant Physiology,1994,106(3):877-886.
[5]宾金华,姜胜,黄胜琴,等.茉莉酸甲酯诱导烟草幼苗抗炭疽病与PAL活性及细胞壁物质的关系[J].植物生理与分子生物学学报,2000,26(1):1-6.
[6]Chen A H,Chai Y R,Li J N,et al.Molecular cloning of two genes encoding cinnamate 4-hydroxylase(C4H)from oilseed rape(Brassica napus)[J].Journal of Biochemistry and Molecular Biology,2007,40(2):247-260.
[7]Kumar S,Omer S,Chitransh S,et al.Cinnamate 4-hydroxylase down-regulation in transgenic tobacco alters transcript level of other phenylpropanoid pathway genes[J].International Journal of Advanced Biotechnology and Research,2012,3(2):545-557.
[8]肖协忠.烟草化学[M].北京:中国农业科技出版社,1997:225-226.
[9]于存峰,张峻松,闰洪洋,等.烟草中多酚类化合物研究进展[J].河南农业科学,2008,37(4):10-14.
[10]朱小茜,徐晓燕,黄义德,等.多酚类物质对烟草品质的影响[J].安徽农业科学,2005,33(10):1910-1911.
[11]周恒,许自成,赵会纳,等.烟草多酚类物质的研究进展[J].浙江农业科学,2009(5):949-953,955.
[12]于利.烤烟苯丙烷代谢关键酶基因分离及表达模式分析[D].北京:中国农业科学院,2014.
[13]王瑞新,闫克玉,韩锦峰,等.烟草化学[M].北京:中国农业出版社,2003.
[14]鲁黎明,杨铁钊.烟草钾转运体基因Nt HAK1的克隆及表达模式分析[J].核农学报,2011,25(3):469-476.
[15]徐晓燕,孙五三,王能如.烟草多酚类化合物的合成与烟叶品质的关系[J].中国烟草科学,2003(1):3-5.
[16]周冀衡,王勇,邵岩,等.产烟国部分烟区烤烟质体色素及主要挥发性香气物质含量的比较[J].湖南农业大学学报(自然科学版),2005,31(2):128-132.
[17]闫克玉.烟草化学[M].郑州:郑州大学出版社,2002:273-274.
[18]Fahrendorf T,Dixon R A.Stress responses in alfalfa(Medicago sativa L.).ⅩⅤⅢ:Molecular cloning and expression of the elicitorinducible cinnamic acid 4-hydroxylase cytochrome P450[J].Archives of Biochemistry and Biophysics,1993,305(2):509-515.
[19]Mizutani M,Ward E,Dimaio J,et al.Molecular cloning and sequencing of a c DNA encoding mung bean cytochrome P450(P450C4H)possessing cinnamate-4-hydroxyla activity[J].Biochemical and Biophysical Research Communications,1993,190(3):875-880.
[20]骆萍,王国栋,陈晓亚.亚洲棉C4H同源c DNA的分离和表达特征分析[J].植物学报(英文版),2001,43(1):77-81.
[21]Williamson R E,Gwynn G R.Variation of polyphenols in fluecured tobacco cultivars attributed to location,stalk position,and year[J].Crop Science,1982,22(1):144-146.