环境胁迫对东方伊萨酵母FJ-J-3产乙醇的影响
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摘要
该研究以东方伊萨酵母(Issatchenkia orientalis)FJ-J-3为研究对象,利用分光光度计法和气相色谱(GC)法测定其在不同pH和不同Na_2SO_4含量胁迫下的生长状况和乙醇产量。结果表明,当pH为2.0、不含Na_2SO_4时,OD_(600 nm)值(4.8)和乙醇产量(1.3%vol)最高;当pH为4.0、Na_2SO_4含量为2%时,OD_(600 nm)值(26.5)和乙醇产量(3.0%vol)最高;当pH为6.0、Na_2SO_4含量为6%时,OD_(600 nm)值(27.5)和乙醇产量(2.9%vol)最高;当pH为8.0、Na_2SO_4含量为2%时,OD_(600 nm)值(20.9)和乙醇产量(2.3%vol)最高。菌株FJ-J-3在pH=4.0和pH=6.0培养条件下具有较好的盐耐受性;在pH=2.0和pH=8.0的条件下,菌株FJ-J-3对盐的耐受性减弱。
Using Issatchenkia orientalis as the research object, the growth situation and ethanol production of I. orientalis FJ-J-3 under different p H and Na_2SO_4 content stresses were determined by spectrophotometry and GC. The results showed that the OD_(600 nm)value(4.8) and ethanol production(1.3%vol) were the highest when the pH was 2.0 and without Na_2SO_4, the OD_(600 nm)value(26.5) and ethanol production(3.0%vol) were the highest when the pH was 4.0 and Na_2SO_4 content was 2%, the OD_(600 nm)value(27.5) and ethanol production(2.9%vol) were the highest when the pH was 6.0 and Na_2SO_4 content was 6%, and the OD_(600 nm)value(20.9) and ethanol production(2.3%vol) were the highest when the pH was 8.0 and Na_2SO_4 content was 2%. The strain FJ-J-3 had good salt tolerance under the culture conditions of pH=4.0 and pH=6.0, while the salt tolerance of strain FJ-J-3 weakened under the culture conditions of pH=2.0 and pH=8.0.
Using Issatchenkia orientalis as the research object, the growth situation and ethanol production of I. orientalis FJ-J-3 under different p H and Na_2SO_4 content stresses were determined by spectrophotometry and GC. The results showed that the OD_(600 nm)value(4.8) and ethanol production(1.3%vol) were the highest when the pH was 2.0 and without Na_2SO_4, the OD_(600 nm)value(26.5) and ethanol production(3.0%vol) were the highest when the pH was 4.0 and Na_2SO_4 content was 2%, the OD_(600 nm)value(27.5) and ethanol production(2.9%vol) were the highest when the pH was 6.0 and Na_2SO_4 content was 6%, and the OD_(600 nm)value(20.9) and ethanol production(2.3%vol) were the highest when the pH was 8.0 and Na_2SO_4 content was 2%. The strain FJ-J-3 had good salt tolerance under the culture conditions of pH=4.0 and pH=6.0, while the salt tolerance of strain FJ-J-3 weakened under the culture conditions of pH=2.0 and pH=8.0.
引文
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[14] ISONO N, HAVAKAWA H, USAMI A, et al. A comparative study of ethanol production by Issatchenkia orientalis strains under stress conditions[J]. J Biosci Bioeng, 2012, 113(1):76-78.
[15] KOUTINAS M, PATSALOU M, STAVRINOU S. et al. High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10[J]. Lett Appl Microbiol, 2016,62(1):75-83.
[16] XIAO H, SHAO Z, JIANG Y. et al. Exploiting Issatchenkia orientalis SD108 for succinic acid production[J]. Microb Cell Factor, 2014, 13(1):121.
[17] MIAO Y, XIONG G, LI R, et al. Transcriptome profling of Issatchenkia orientalis under ethanol stress[J]. AMB Expr, 2018, 8:39.
[18]姚淑敏,徐凯峰,宋晴晴,等.东方伊萨酵母FJ-J-3发酵特性的研究[J].中国酿造,2017,36(12):92-95.
[19]杨革.微生物学实验教程(第三版)[M].北京:科学出版社,2018:145-146
[20]陈国通,毛琼玲,左书瑞,等.气相色谱法测定低酒精度饮料中乙醇的含量[J].食品与机械,2018,34(2):58-61.
[2] OLOFSSON K, BERTILSSON M, GUNNAR L, et al. A short review on SSF-an interesting process option for ethanol production from lignocellulosic feedstocks[J]. Biotechnol Biofuels, 2008, 1(1):7.
[3] BALAT M, BALAT H,?Z C. Progress in bioethanol processing[J]. Prog Energ Combust, 2008, 34(5):551-573.
[4]彭源德,朱作华,唐守伟,等.耐高温、高浓度酒精酵母的选育与耐受性能初步鉴定[J].中国麻业科学,2010,32(3):135-142.
[5] HOHMANN S, MAGER W H. Yeast stress responses[J]. Febs J, 1997,272(11):2639-2647.
[6] CARMELO V, BOGAERTS P, S魣-CORREIA I, et al. Activity of plasma membrane H+-ATPase and expression of PMA1 and PMA2 genes in Saccharomyces cerevisiae cells grown at optimal and low pH[J]. Arch Microbiol, 1996, 166(5):315-230.
[7]熊国通,苗英杰,吴祖芳,等.乙醇胁迫下酿酒酵母(Sc131)生长状态和基因的表达[J].中国食品学报,2018,18(8):247-253.
[8]李莉莉,叶美莉,叶燕锐,等.酿酒酵母乙醇发酵性能与胁迫耐性的相关性[J].华南理工大学学报,2011,39(9):134-139.
[9]李王强,刘月,孟祥栓,等.新疆塔城地区乳源酵母菌的分离、鉴定及其抗胁迫特性[J].食品工业科技,2018,39(22):99-106.
[10] VASKAR M, DOROTA R, GUIDO A. Phenotypic landscape of nonconventional yeast species for different stress tolerance traits desirable in bioethanol fermentation[J]. Biotechnol Biofuels, 2017, 10(1):216.
[11] LI C, YU J, WANG D, et al. Efficient removal of zinc by multi-stresstolerant yeast Pichia kudriavzevii A16[J]. Bioresource Technol, 2016,206:43-49.
[12] LI C, LI L, YANG X, et al. Effect of inorganic salt stress on the thermotolerance and ethanol production at high temperature of Pichia kudriavzevii[J]. Ann Microbiol, 2018, 68(5):305-312.
[13] KURTZMAN, ROBNET C P, BASEHOARPOWERS C J. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen.nov., Lindnera gen. nov. and Wickerhamomyces gen. nov.[J]. Fems Yeast Res, 2008, 8(6):939-954.
[14] ISONO N, HAVAKAWA H, USAMI A, et al. A comparative study of ethanol production by Issatchenkia orientalis strains under stress conditions[J]. J Biosci Bioeng, 2012, 113(1):76-78.
[15] KOUTINAS M, PATSALOU M, STAVRINOU S. et al. High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10[J]. Lett Appl Microbiol, 2016,62(1):75-83.
[16] XIAO H, SHAO Z, JIANG Y. et al. Exploiting Issatchenkia orientalis SD108 for succinic acid production[J]. Microb Cell Factor, 2014, 13(1):121.
[17] MIAO Y, XIONG G, LI R, et al. Transcriptome profling of Issatchenkia orientalis under ethanol stress[J]. AMB Expr, 2018, 8:39.
[18]姚淑敏,徐凯峰,宋晴晴,等.东方伊萨酵母FJ-J-3发酵特性的研究[J].中国酿造,2017,36(12):92-95.
[19]杨革.微生物学实验教程(第三版)[M].北京:科学出版社,2018:145-146
[20]陈国通,毛琼玲,左书瑞,等.气相色谱法测定低酒精度饮料中乙醇的含量[J].食品与机械,2018,34(2):58-61.