冬虫夏草联合丹参器官保存液对大鼠肝脏保存效果的研究
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摘要
目的分析研究冬虫夏草联合丹参器官保存液对大鼠肝脏保存效果。方法选取2016年3月—2017年3月该院进行肝脏移植试验的86只SD大鼠作为该次研究对象,随机数字表达法分为43例研究组和43例对照组,其中对照组中的SD大鼠采用UW保存液对其肝脏进行保存,研究组中的SD大鼠用观察两组SD大鼠用冬虫夏草联合丹参器官保存液对其肝脏进行保存。结果研究组保存液p H值在(7.41±0.56)左右,渗透压为(325.09±11.03)mmol/L,与对照组中保存液p H值(7.38±0.08)和渗透压(320.15±10.95)mmol/L相比无明显区别(t=3.294,7.391,P>0.05);研究组中离体大鼠所分泌的胆汁量在48 h内随时间的延长逐渐减少[(1.97±0.12),(2.23±0.13),(2.67±0.21),(2.86±0.09),(3.17±0.09)μL],且显著低于对照组中大鼠分泌的胆汁量(1.67±0.03,(2.34±0.21),(2.75±0.11),(3.01±0.04),(3.28±0.56)μL](t=13.294,13.391、15.125、17.935、15.693,P<0.05)。结论就对离体SD大鼠肝脏的保存效果来看,研制的冬虫夏草联合丹参器官保存液与UW液保存效果相近,在灌注后肝细胞胆汁分泌方面略优于UW液,冬虫夏草联合丹参器官保存液对SD大鼠肝脏的保存效果较好,在临床上值得进一步推广。
Objective To analyze the effects of Cordyceps sinensis combined with Danshen organ preservation solution on rat liver preservation. Methods 86 Sprague Dawley rats undergoing liver transplantation in the hospital from March2016 to March 2017 were selected as the study subjects. They were randomly divided into 43 study groups and 43 control groups, among which SD rats in the control group UW preservation solution was used to preserve the liver. SD rats in the study group were observed for two groups of SD rats using Cordyceps sinensis combined with Danshen organ preservation solution to preserve the liver. Results The p H value of the preservation solution in the study group was about(7.41±0.56), and the osmotic pressure was(325.09±11.03)mmol/L. There was no significant difference between the storage solution p H value of(7.38 ±0.08) and the osmotic pressure(320.15 ±10.95)mmol/L in the control group(t =3.294, 7.391, P>0.05); The amount of bile secreted by isolated rats in the study group gradually decreased over time within 48 hours [(1.97±0.12),(2.23±0.13),(2.67±0.21),(2.86±0.09),(3.17±0.09)μL], and was significantly lower. The amount of bile secreted by the rats in the control group [(1.67±0.03)),(2.34±0.21),(2.75±0.11),(3.01±0.04),(3.28±0.56)μL](t=13.294, 13.391, 15.125, 17.935, 15.693, P<0.05). Conclusion For the preservation effect of isolated SD rat liver, the developed Cordyceps sinensis combined with Salvia miltiorrhiza organ preservation solution is similar to that of UW solution, and it is slightly better than UW solution in the secretion of hepatocyte bile after perfusion. Cordyceps sinensis combined with Salvia miltiorrhiza organ preservation solution effect of SD rat liver is better, and it is worthy of further promotion in clinical practice.
Objective To analyze the effects of Cordyceps sinensis combined with Danshen organ preservation solution on rat liver preservation. Methods 86 Sprague Dawley rats undergoing liver transplantation in the hospital from March2016 to March 2017 were selected as the study subjects. They were randomly divided into 43 study groups and 43 control groups, among which SD rats in the control group UW preservation solution was used to preserve the liver. SD rats in the study group were observed for two groups of SD rats using Cordyceps sinensis combined with Danshen organ preservation solution to preserve the liver. Results The p H value of the preservation solution in the study group was about(7.41±0.56), and the osmotic pressure was(325.09±11.03)mmol/L. There was no significant difference between the storage solution p H value of(7.38 ±0.08) and the osmotic pressure(320.15 ±10.95)mmol/L in the control group(t =3.294, 7.391, P>0.05); The amount of bile secreted by isolated rats in the study group gradually decreased over time within 48 hours [(1.97±0.12),(2.23±0.13),(2.67±0.21),(2.86±0.09),(3.17±0.09)μL], and was significantly lower. The amount of bile secreted by the rats in the control group [(1.67±0.03)),(2.34±0.21),(2.75±0.11),(3.01±0.04),(3.28±0.56)μL](t=13.294, 13.391, 15.125, 17.935, 15.693, P<0.05). Conclusion For the preservation effect of isolated SD rat liver, the developed Cordyceps sinensis combined with Salvia miltiorrhiza organ preservation solution is similar to that of UW solution, and it is slightly better than UW solution in the secretion of hepatocyte bile after perfusion. Cordyceps sinensis combined with Salvia miltiorrhiza organ preservation solution effect of SD rat liver is better, and it is worthy of further promotion in clinical practice.
引文
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[11]林惠,杨万章.丹参注射液联合rh G-CSF对脑梗死大鼠神经功能的影响[J].中医药导报,2017,67(17):28-31.
[12]黄丹,段钰萍,王学东,等.丹参川芎嗪注射液对严重创伤患者炎性因子影响及预防脓毒症效果[J].现代中西医结合杂志,2017,26(10):1069-1071.
[2]李文佳,董彩虹,刘杏忠,等.冬虫夏草培植技术研究进展[J].菌物学报,2016,35(4):375-387.
[3]李慧,汪根树.肝硬化肝移植术后脾功能亢进的研究进展[J].器官移植,2016,7(3):238-240.
[4]王立娟.浅析丹参和冬虫夏草制剂用于肾移植中的临床分析[J].世界最新医学信息文摘,2016,16(26):90-91.
[5]费晓冬.丹参等11种中药金属元素含量测定及聚类分析[J].光明中医,2017,32(14):2042-2044.
[6]徐博,王甜,郭伟,等.不同剂量丹参联合川芎嗪对肺纤维化大鼠的保护作用[J].西部医学,2017,58(10):1364-1368.
[7]施少军,郑建新,秦天,等.改良大鼠离体肝脏灌注模型在供肝冷保存中的应用研究[J].胃肠病学,2017,22(9):331-342.
[8]陈规划,姜楠.肝移植免疫耐受的研究热点和思考[J].器官移植,2017,8(1):1-4.
[9]张佳伟,黄建钊,赵鹏伟,等.冬虫夏草在大鼠离体肝脏保存中的实验研究[J].中国民族民间医药,2013,22(2):12-14.
[10]戴晨,王顺,徐加波,等.人工虫草复合液对无心跳模型大鼠离体肾的低温保护作用[J].中国组织工程研究,2016,20(5):664-670.
[11]林惠,杨万章.丹参注射液联合rh G-CSF对脑梗死大鼠神经功能的影响[J].中医药导报,2017,67(17):28-31.
[12]黄丹,段钰萍,王学东,等.丹参川芎嗪注射液对严重创伤患者炎性因子影响及预防脓毒症效果[J].现代中西医结合杂志,2017,26(10):1069-1071.