稀土铕双功能螯合剂的合成
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摘要
均相时间荧光免疫分析(HTRF)是一种快速、简便的分析方法,它将荧光共振能量转移与时间分辨荧光相结合,以稳定的穴状稀土螯合剂为荧光标记物进行检测分析。为了满足HTRF的分析要求,以2,6-二甲基吡啶-4-羧酸甲酯为原料,经N-溴代琥珀酰亚胺(NBS)溴化、水解等化学反应制备得到Eu~(3+)-双功能螯合剂2,6-{N,N',N,N'-[二(2,2’-联吡啶-6,6’-二甲基)]二氨甲基}-吡啶-4-羧酸-Eu~(3+),通过IR、MS等表征方法证明各化合物结构的准确性。对螯合剂荧光性质进行研究。螯合剂的最大激发波长为320nm;最大发射波长为597nm (~5D_0-~7F_2),Stokes位移达到277nm;荧光寿命828μs;荧光量子产率Y_f=0.233。其荧光性质可以满足HTRF的分析要求。
Homogeneous-Time Fluorescence Immunoassay(HTRF) was a simple,rapid analysis method being combined fluorescence resonance energy transfer with time-resolved fluorescence,and a stable cryptate rare earth chelator was used as a fluorescent marker. In order to meet the requirements of HTRF,the 2,6-bis methyl pyridine-4-carboxylic ester as starting material via NBS bromination,Eu~(3+)bi-functional chelator2,6-{N,N',N,N'-[bis(2,2'–bi-pyridine-6,6'-dimethyl) bis(aminomethyl)]-pyridine}-4-carboxylic ester-Eu~(3+)was finally prepared by hydrolysis reaction and other chemical reactions. The structure and purity of compounds was confirmed by IR,MS and other characterization methods. The fluorescence properties of the chelating agent showed that the maximum excitation wavelength was 320 nm,the maximum emission wavelength was 597 nm(~5 D_0-~7 F_2),the Stokes displacement was 277 nm,the fluorescence lifetime was 828μs, and the fluorescence quantum yield Y_f=0.233. Its fluorescence properties can meet the analysis requirements of HTRF
Homogeneous-Time Fluorescence Immunoassay(HTRF) was a simple,rapid analysis method being combined fluorescence resonance energy transfer with time-resolved fluorescence,and a stable cryptate rare earth chelator was used as a fluorescent marker. In order to meet the requirements of HTRF,the 2,6-bis methyl pyridine-4-carboxylic ester as starting material via NBS bromination,Eu~(3+)bi-functional chelator2,6-{N,N',N,N'-[bis(2,2'–bi-pyridine-6,6'-dimethyl) bis(aminomethyl)]-pyridine}-4-carboxylic ester-Eu~(3+)was finally prepared by hydrolysis reaction and other chemical reactions. The structure and purity of compounds was confirmed by IR,MS and other characterization methods. The fluorescence properties of the chelating agent showed that the maximum excitation wavelength was 320 nm,the maximum emission wavelength was 597 nm(~5 D_0-~7 F_2),the Stokes displacement was 277 nm,the fluorescence lifetime was 828μs, and the fluorescence quantum yield Y_f=0.233. Its fluorescence properties can meet the analysis requirements of HTRF
引文
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[2] Kaes C,Katz A,Hosseini M W. ChemInform abstract:bipyridine:the most widely used ligand. a review of molecules comprising at least two 2,2′-bipyridine units[J]. Chemical Reviews,2000,100(10):3553-3590.
[3]常宇,谢文兵,周誓红,等.稀土穴状荧光配合物的合成与光谱性质[J].应用化学,2017,34(3):361-366.
[4] Jiang L,Wu J,Wang G,et al. Development of a visible-light-sensitized europium complex for time-resolved fluorometric application[J]. Analytical Chemistry,2010,82(6):2529-2531.
[5] Smeulders L,Bunkens L,Vereycken I,et al. A homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion[J].Methods Mol Biol,2013,1030(6):11-17.
[6]孔令艳.固相时间分辨荧光免疫分析双功能螯合剂中间体的合成[J].呼伦贝尔学院学报,2007,15(6):81-84.
[7] Degorce F,Card A,Soh S,et al. HTRF:A technology tailored for drug discovery-a review of theoretical aspects and recent applications[J]. Current Chemical Genomics,2009,3(1):22-32.
[8]王慧洁,周子涵,徐嘉辰,等.基于均相时间分辨荧光技术(HTRF)的HSP90-HOP相互作用抑制剂活性测试方法的构建及其应用[J].药学学报,2017(4):592-597.
[9] Chaudhry C,Davis J,Zhang Y,et al. Building homogeneous time-resolved fluorescence resonance energy transfer assays for characterization of bivalent inhibitors of an inhibitor of apoptosis protein target.[J]. Analytical Biochemistry,2016(497):8-17.
[10] Stowell A I,James D I,Waddell I D,et al. A high-throughput screening-compatible homogeneous time-resolved fluorescence assay measuring the glycohydrolase activity of human poly(ADP-ribose)glycohydrolase[J]. Analytical Biochemistry,2016(503):58-64.
[11] Chen Y K,Bonaldi T,Cuomo A,et al. Design of KDM4 Inhibitors with Antiproliferative Effects in Cancer Models.[J]. Acs Medicinal Chemistry Letters,2017,8(8):869-874.
[12] Kim S M,Chae M K,Yim M S,et al. Hybrid PET/MR imaging of tumors using an oleanolic acid-conjugated nanoparticle[J]. Biomaterials,2013,34(33):8114-8121.
[13]朱国贤,李永大,王兴权.YF3:Eu3+荧光体的合成和光谱性质[J].长春理工大学学报:自然科学版,2009,32(3):410-412.