达氟沙星对猪胸膜肺炎放线杆菌的体外药效研究
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摘要
为了解猪胸膜肺炎放线杆菌对达氟沙星的敏感性和达氟沙星的杀菌效果,收集和分离48株猪胸膜肺炎放线杆菌,采用微量肉汤稀释法和菌落计数法对体外药物敏感性试验和生长曲线、杀菌曲线进行了研究。猪胸膜肺炎放线杆菌对氨苄西林、磺胺异恶唑、头孢噻呋、大观霉素、黏菌素耐药率高,对阿莫西林/克拉维酸、复方新诺明、氟苯尼考、多西环素、庆大霉素、四环素、恩诺沙星、氧氟沙星、达氟沙星敏感。达氟沙星最小抑菌浓度(MIC)集中在0.0078μg/m L。达氟沙星浓度在MIC及以上时,随浓度升高,杀菌时间缩短,为典型的浓度依赖型抗生素。该试验结果可用于指导猪传染性胸膜肺炎的临床预防和治疗。
To understand the sensitivity of Actinobacillus pleuropneumonia to danofloxacin and the effect of danofloxacin,48 strains are selected and isolated to investigate the drug sensitivity test,growth curve and killing curve in vitro by micro broth dilution method and colony counting method. The results showed that the pathogen are resistant to ampicillin,sulfamethoxazole,ceftiofur,spectinomycin and colistin and sensitive to amoxicillin/clavulanic acid, compound sulfamethoxazole/TMP, florfenicol, doxycycline, gentamicin, tetracycline,enrofloxacin,ofloxacin and danofloxacin. The minimum inhibitory concentration of danofloxacin is 0.0078 μg/m L.When the concentration of danofloxacin is at MIC and above,the sterilization time is shortened with the increase of concentration. It is a typical concentration dependent antibiotic. These findings can assist in the clinical prevention and the treatment of porcine contagious pleuropneumonia.
To understand the sensitivity of Actinobacillus pleuropneumonia to danofloxacin and the effect of danofloxacin,48 strains are selected and isolated to investigate the drug sensitivity test,growth curve and killing curve in vitro by micro broth dilution method and colony counting method. The results showed that the pathogen are resistant to ampicillin,sulfamethoxazole,ceftiofur,spectinomycin and colistin and sensitive to amoxicillin/clavulanic acid, compound sulfamethoxazole/TMP, florfenicol, doxycycline, gentamicin, tetracycline,enrofloxacin,ofloxacin and danofloxacin. The minimum inhibitory concentration of danofloxacin is 0.0078 μg/m L.When the concentration of danofloxacin is at MIC and above,the sterilization time is shortened with the increase of concentration. It is a typical concentration dependent antibiotic. These findings can assist in the clinical prevention and the treatment of porcine contagious pleuropneumonia.
引文
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[2] Cockerill F R. Performance standards for antimicrobial susceptibility testing:twentieth informational supplement[M]. Pennsylvania:Clinical and Laboratory Standards Institute,2013.
[3] Sassu E L,Ladinig A,Talker S C,et al. Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae[J]. Veterinary Research,2017,48(1):4.
[4]吴根福,吴雪昌,余斌,等.大观霉素的研究进展及其在畜牧业中的应用[J].中国兽药杂志,2005,39(9):29-32.Wu G F,Wu X C,Y B,et al. Progress on Sectinomycin and its application in animal husbandry[J]. Chinese Journal of Veterinary Drug,2005,39(9):29-32.
[5]张云峰,康立超,王静梅.猪胸膜肺炎放线杆菌病原的分离与鉴定[J].山东畜牧兽医,2007,30(3):33-33.Zhang Y F,Kang L C,Wang J M. Isolation and identification of Actinobacillus pleuropneumoniae[J]. Shangdong Journal of Animal Science and Veterinary Medicine,2007,30(3):33.
[6]倪莉,徐强,童永亮,等.猪繁殖与呼吸综合征病毒和胸膜肺炎放线杆菌混合感染的诊治[J].黑龙江畜牧兽医,2011,(3):105-106.Ni L,Xu Q,Tong Y L,et al. Diagnosis and treatment of mixed infection of porcine reproductive and respiratory syndrome virus and Actinobacillus pleuropneumoniae[J]. Heilongjiang Journal of Animal Science and Veterinary Medicine,2011,(3):105-106.
[7] Giles C J,Magonigle R A,Grimshaw W T,et al. Clinical pharmacokinetics of parenterally administered danofloxacin in cattle[J].Journal of Veterinary Pharmacology&Therapeutics,1991,14(4):400.
[8] Turnidge J,Paterson D L. Setting and revising antibacterial susceptibility breakpoints[J]. Clinical Microbiology Reviews,2007,20(3):391-408.