刺五加苷E对穹窿-海马伞损伤大鼠学习记忆能力及海马神经元可塑性的影响
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摘要
目的探索刺五加苷E对穹窿-海马伞损伤大鼠学习记忆能力及海马神经元可塑性的影响。方法 120只大鼠随机分为对照组、模型组、假手术组及刺五加苷E低、中、高剂量组(100、200、500 mg/kg),每组20只。第7、21天分别测定寻找平台潜伏期和错误次数后,RT-PCR、ELISA检测锥体细胞凋亡率、c-fos基因表达、Na+-K+-ATP酶活性。结果与模型组比较,刺五加苷E组寻找平台潜伏期、错误次数显著减少(P <0. 05),Na+-K+-ATP酶活性、正常锥体细胞数量显著增加(P <0. 05),锥体细胞密集度和排列方式、神经纤维缠结、胶质细胞增生、核固缩明显改善(P <0. 05),第7天c-fos相对灰度值显著增加(P <0. 05),第21天恰好相反(P <0. 05)。结论刺五加苷E可剂量依赖性地改善穹窿-海马伞损伤大鼠学习记忆能力,其机制可能与增强Na+-K+-ATP酶活性、调控c-fos基因表达、阻止海马神经元损伤并促进其可塑性有关。
AIM To explore the effects of eleutheroside E on memory and learning activities,hippocampal neuron plasticity of rats with fornix-fimbria lesions. METHODS One hundred and twenty rats were randomly divided into control group,model group,sham operation group and eleutheroside E low-dose,medium-dose,highdose groups(100,200,500 mg/kg),20 rats in each group. After the determination of searching platform latency and error frequency on the 7 thand 21 stdays,respectively,RT-PCR and ELISA were applied to detecting pyramidal cell apoptosis rate,c-fos gene expression and Na+-K+-ATPase activity. RESULTS Compared with the model group,the eleutheroside E groups demonstrated significantly reduced searching platform latency and error frequency(P < 0. 05),markedly increased Na+-K+-ATPase activity and normal pyramidal cell number(P < 0. 05),obviously improved pyramidal cell intensity and arrangement style,neurofibrillary tangles,glial cell proliferation,karyopyknosis(P < 0. 05),and c-fos relative gray value was significantly increased on the 7 thday(P < 0. 05),which was just on the contrary on the 21 stday(P < 0. 05). CONCLUSION Eleutheroside E can improve the memory and learning activities of rats with fornix-fimbria lesions in a dose-dependent manner,whose mechanisms may be related to enhancing Na+-K+-ATPase activity,regulating c-fos gene expression,preventing hippocampal neuron damage and promoting its plasticity.
AIM To explore the effects of eleutheroside E on memory and learning activities,hippocampal neuron plasticity of rats with fornix-fimbria lesions. METHODS One hundred and twenty rats were randomly divided into control group,model group,sham operation group and eleutheroside E low-dose,medium-dose,highdose groups(100,200,500 mg/kg),20 rats in each group. After the determination of searching platform latency and error frequency on the 7 thand 21 stdays,respectively,RT-PCR and ELISA were applied to detecting pyramidal cell apoptosis rate,c-fos gene expression and Na+-K+-ATPase activity. RESULTS Compared with the model group,the eleutheroside E groups demonstrated significantly reduced searching platform latency and error frequency(P < 0. 05),markedly increased Na+-K+-ATPase activity and normal pyramidal cell number(P < 0. 05),obviously improved pyramidal cell intensity and arrangement style,neurofibrillary tangles,glial cell proliferation,karyopyknosis(P < 0. 05),and c-fos relative gray value was significantly increased on the 7 thday(P < 0. 05),which was just on the contrary on the 21 stday(P < 0. 05). CONCLUSION Eleutheroside E can improve the memory and learning activities of rats with fornix-fimbria lesions in a dose-dependent manner,whose mechanisms may be related to enhancing Na+-K+-ATPase activity,regulating c-fos gene expression,preventing hippocampal neuron damage and promoting its plasticity.
引文
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[2] Huang D B,Hu Z H,Yu Z F. Eleutheroside B or E enhances learning and memory in experimentally aged rats[J]. Neural Regen Res,2013,8(12):1103-1112.
[3]陈奔,覃梅春,黄金兰,等.老年性痴呆-血瘀证病证结合树鼩模型的初步建立及三七总皂苷干预的评价[J].中国中药杂志,2017,42(6):1175-1182.
[4]吴建华,杜银香,黄强.刺五加苷B/E对高糖环境下HBZY-1细胞增殖及TGF-β1和PPARγ表达的影响[J].中国病理生理杂志,2015,31(3):511-517.
[5]王合作,孙燕,李立,等.刺五加注射液联合氟西汀治疗老年痴呆伴发抑郁的疗效分析[J].国际精神病学杂志,2016,43(1):116-118.
[6]第五永长,田金洲,时晶.洗心汤对散发性老年性痴呆大鼠脑内tau蛋白O-Glc NAc糖基化修饰的影响[J].中成药,2013,35(8):1620-1625.
[7]颜永刚,王红艳,尹立敏,等.太白洋参不同溶剂提取物对老年性痴呆模型大鼠TNF-α、CRP、5-HT、AChE的影响[J].中成药,2016,38(4):908-911.
[8]元红花,程琳,许妍姬.刺五加皂苷对Aβ25~35诱发PC12细胞毒性及细胞凋亡的保护作用[J].中国老年学杂志,2014,34(6):1570-1572.
[9]孟锐,陈逸青,陈勤.阿魏酸对痴呆小鼠脑内胶质细胞活化与炎性细胞因子表达的影响[J].中国医院药学杂志,2018,38(1):50-53.
[10]余圆圆,陈钧.微波炮制白果对APP/PS1转基因老年痴呆小鼠学习记忆能力的影响[J].中成药,2018,40(1):1-7.
[11] Kobayashi D,Yoshimura T,Johno A,et al. Decrease in pyridoxal-5'-phosphate concentration and increase in pyridoxal concentration in rat plasma by 4'-O-methylpyridoxine administration[J]. Nutr Res,2015,35(7):637-642.
[12] Kobayashi D,Yoshimura T,Johno A,et al. Toxicity of 4'-Omethylpyridoxine-5'-glucoside in Ginkgo biloba seeds[J]. Food Chem,2011,126(3):1198-1202.
[13]周桂生,姚鑫,唐于平,等.白果仁化学成分研究[J].中国药学杂志,2012,47(17):1362-1366.
[14]姚刚,朱博驰,于挺敏,等.丁苯酞对血管性痴呆大鼠海马CA1区Bax和Bcl-2表达的影响[J].中国老年学杂志,2018,38(1):6-10.