微量元素配施对甜菊生长和甜菊糖苷含量的影响
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摘要
采用正交试验设计,以当年生甜菊扦插苗为试材进行盆栽试验,研究锌(Zn)、硼(B)、锰(Mn)、钼(Mo)配施对幼苗生长和糖苷含量的影响。结果表明,合理配施Zn、B、Mn、Mo能促进甜菊生长发育,施锌能显著影响甜菊茎粗,施钼则显著影响甜菊叶片中叶绿素a、叶绿素b和总叶绿素含量;适宜配比的Zn、B、Mn、Mo对甜菊糖苷含量也有积极的调节作用,Zn 0.1%、B 0.1%、Mn 0.3%和Mo 0%的组合处理下甜菊叶片中R-A苷和总甜菊糖苷含量均最高,Zn 0.2%、B 0%、Mn 0.2%和Mo 0.3%的组合处理使甜菊叶片中R-A/总苷的比率最高。
The pot culture experiment was conducted with the 1-year-old cutting seedlings by borthogonal experiment design to study the effects of microelement fertilizers(zinc,boron,manganese and molybdenum) on growth and steviol glycosides content of stevia.The results showed that increment microelements could promote the growth of stevia plant.Especially,zinc could increase stem diameter significantly,and molybdenum could increase the contents of chlorophyll a,chlorophyll b and total chlorophyll in stevia leaves.Meanwhile,the proportional zinc,boron,manganese and molybdenum fertilizers could regulate positively the accumulation of steviol glycosides in leaves.When applying Zn 0.1%,B 0.1%,Mn 0.3% and Mo 0%,the content of rebaudioside A(R-A) and total steviol glycosides were maximum in all treatment groups.And when increased Zn 0.2%,B 0%,Mn 0.2% and Mo 0.3% into soils,the rate of R-A/total steviol glycosides was the highest.
The pot culture experiment was conducted with the 1-year-old cutting seedlings by borthogonal experiment design to study the effects of microelement fertilizers(zinc,boron,manganese and molybdenum) on growth and steviol glycosides content of stevia.The results showed that increment microelements could promote the growth of stevia plant.Especially,zinc could increase stem diameter significantly,and molybdenum could increase the contents of chlorophyll a,chlorophyll b and total chlorophyll in stevia leaves.Meanwhile,the proportional zinc,boron,manganese and molybdenum fertilizers could regulate positively the accumulation of steviol glycosides in leaves.When applying Zn 0.1%,B 0.1%,Mn 0.3% and Mo 0%,the content of rebaudioside A(R-A) and total steviol glycosides were maximum in all treatment groups.And when increased Zn 0.2%,B 0%,Mn 0.2% and Mo 0.3% into soils,the rate of R-A/total steviol glycosides was the highest.
引文
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[15]李焕忠,张吉立.5种微量元素对紫叶矮樱叶绿素a含量影响的研究[J].中国农学通报,2010,26(16):242-245.
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[17]曹秀霞,安维太,万海霞.硼肥对旱地胡麻生长及产量的影响[J].北方园艺,2015(6):154-157.
[18]刘武定.微量元素营养与微肥施用[M].北京:农业出版社,1995.
[19]聂兆君.钼对小白菜抗坏血酸代谢及品质的影响[D].武汉:华中农业大学,2007.
[2]Geeraert B,CrombéF,Hulsmans M,et al.Stevioside inhibits atherosclerosis by improving insulin signaling and antioxidant defense in obese insulin-resistant mice[J].International Journal of Obesity,2010,34(3):569-577.
[3]Akbarzadeh S,Eskandari F,Tangestani H,et al.The effect of Stevia rebaudiana on serum omentin and visfatin level in STZinduced diabetic rats[J].Journal of Dietary Supplements,2015,12(1):11-22.
[4]Ferri L A F,Alves‐Do‐Prado W,Yamada S S,et al.Investigation of the antihypertensive effect of oral crude stevioside in patients with mild essential hypertension[J].Phytotherapy Research Ptr,2006,20(9):732-736.
[5]Jeppesen P B,Gregersen S,Rolfsen S E,et al.Antihyperglycemic and blood pressure-reducing effects of stevioside in the diabetic Goto-Kakizaki rat.[J].Metabolism Clinical&Experimental,2003,52(3):372-378.
[6]Chatsudthipong V,Muanprasat C.Stevioside and related compounds:Therapeutic benefits beyond sweetness[J].Pharmacology&Therapeutics,2009,121(1):41-54.
[7]Takasaki M,Konoshima T M,Tokuda H,et al.Cancer preventive agents.Part 8:Chemopreventive effects of stevioside and related compounds[J].Bioorganic&Medicinal Chemistry,2009,17(2):600-605.
[8]胡霭堂,陆景陵.植物营养学:第2版[M].北京:中国农业大学出版社,2003.
[9]王晓菁,牛艳,王彩艳,等.铁、锌喷施对宁夏枸杞中有效成分、微量元素含量的影响[J].北方园艺,2015(7):153-156.
[10]胡华锋,介晓磊,刘世亮,等.微肥配施对紫花苜蓿生产效应的研究[J].甘肃农大学报,2007,42(3):85-90.
[11]孟杰,陈兴福,杨文钰,等.锌硼钼配施对北柴胡产量及柴胡皂苷a,d含量的影响[J].中国中药杂志,2014,39(22):4297-4303.
[12]曾小燕.栽培措施对甜菊品质影响的研究[D].福州:福建农林大学,2011.
[13]包亚英,胡秀英,郝雨杉,等.不同施肥处理对甜菊生长及糖苷含量和积累量的影响[J].植物资源与环境学报,2016,25(1):71-80.
[14]Yang Y,Huang S,Han Y,et al.Environmental cues induce changes of steviol glycosides contents and transcription of corresponding biosynthetic genes in Stevia rebaudiana[J].Plant Physiology&Biochemistry Ppb,2015,86(86C):174-180.
[15]李焕忠,张吉立.5种微量元素对紫叶矮樱叶绿素a含量影响的研究[J].中国农学通报,2010,26(16):242-245.
[16]王苏影,颜廷献,徐宝庆,等.配施硼、锌肥及施用方法对芝麻生长及产量的影响[J].江西农业学报,2014(12):35-37.
[17]曹秀霞,安维太,万海霞.硼肥对旱地胡麻生长及产量的影响[J].北方园艺,2015(6):154-157.
[18]刘武定.微量元素营养与微肥施用[M].北京:农业出版社,1995.
[19]聂兆君.钼对小白菜抗坏血酸代谢及品质的影响[D].武汉:华中农业大学,2007.