摘要
黄曲霉毒素大量存在于花生、玉米、稻米、大豆、小麦和茶叶中,其毒性、致癌力在霉菌毒素中都居首位,是对人类健康危害极为突出的一类霉菌毒素.本研究使用上转换纳米材料为荧光标记物,在其表面修饰可特异性地结合黄曲霉毒素B1的核酸适配体,构建出一种基于竞争法的荧光侧流试纸检测技术.该技术可实现对黄曲霉毒素B1的快速检测,在0.1~100 ng/mL的浓度范围内具有良好的线性度,最低检出限为0.03 ng/mL,并实现了对实际茶水样品的定量检测.该方法灵敏度高、特异性强、适用于现场的快速检测,具有良好的经济价值和应用前景.
Aflatoxin, extensively existing in peanuts, corn, rice, soybeans, wheat and tea, is a type of mycotoxins with the highest toxicity and carcinogenicity and thus poses a serious threat to human health. In this article, upconversion nanoparticles modified with aptamers specific to aflatoxin B1 were used as fluorescence markers. By this way, we constructed a paper-based lateral flow assays detection technology for rapid detection of aflatoxin B1 with good linearityin the concentration range of 0.1–100 ng/mL and detection limit of 0.03 ng/mL. This method can realize quantitative detection of aflatoxin B1 in real tea sample with high sensitivity and specificity. Hence it is suitable for rapid on-site detection of aflatoxin B1 by utilizing this method, which shows good economic value and application prospects.
引文
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