两种γ计数器测量PTH(1-84和1-34)体外药代的应用研究
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摘要
目的通过两种γ计数器测量注射用重组人甲状旁腺素PTH(1-84)及其代谢产物PTH(1-34)体外药代实验的测量数据,找出进口与国产γ计数器在实际测量中的差异。方法用PE公司Wallac1470 WIZARD与中佳GC-1200γ计数器分别测定同一份PTH样品的cpm,分别建立标准曲线,对仪器的本底计数、相对测量效率、精密度等性能指标进行比较。结果 Wallac1470 WIZARD与GC-1200γ计数器测量PTH(1~84)cpm值的线性关系良好;PE-1470本底值为14±3 cpm、GC-1200本底值为57±33 cpm;PE-1470对~(125)I相对测量效率比GC-1200高出12%左右。结论 PE公司Wallac1470 WIZARD与中佳GC-1200γ计数器操作简单方便;PE-1470的准确、灵敏度略优,两者都适用于放射性体外药代样品的测定。
Objective The measurement data of the recombinant human parathyroid hormone PTH( 1-84) and its metabolite( 1-34) in vitro of the recombinant human parathyroid hormone( PTH) were measured by two kinds of gamma counter,and the difference between the imported and domestic gamma counter was found. Methods Cpm of the some PTH samples were determined with a PTH sample cpm by PE company Wallac 1470 WIZARD and Ustc ZonKia GC-1200 γ counter respectively to establish the standard curve of the instrument,the background count,relative measurement efficiency,precision and other performance indicators were compared. Results Wallac1470 WIZARD and GC-1200 gamma counter measuring PTH( 1-84) linear relationship between the cpm value is good,the regression equation was : Y( cpm) = 25. 371x( concentration,R~2= 0. 9982) and Y( cpm) = 19. 181 x( concentration,R~2= 0. 998); PE-1470 the background value is 14 ± 3( cpm),GC-1200 as the background value 57 ± 33( cpm); PE-1470 to~(125)I relative measurement efficiency is about 12% higher than GC-1200; PE-1470 and GC-1200 measurement precision is RSD = 0. 72% and RSD = 1. 42% respectively. Conclusion PE Wallac1470 WIZARD and Ustc Zon Kia GC-1200 γ counter were simple and convenient to operate,accuracy and sensitivity of PE-1470 were slightly better. Both are applicable to radioactive drug generation samples.
Objective The measurement data of the recombinant human parathyroid hormone PTH( 1-84) and its metabolite( 1-34) in vitro of the recombinant human parathyroid hormone( PTH) were measured by two kinds of gamma counter,and the difference between the imported and domestic gamma counter was found. Methods Cpm of the some PTH samples were determined with a PTH sample cpm by PE company Wallac 1470 WIZARD and Ustc ZonKia GC-1200 γ counter respectively to establish the standard curve of the instrument,the background count,relative measurement efficiency,precision and other performance indicators were compared. Results Wallac1470 WIZARD and GC-1200 gamma counter measuring PTH( 1-84) linear relationship between the cpm value is good,the regression equation was : Y( cpm) = 25. 371x( concentration,R~2= 0. 9982) and Y( cpm) = 19. 181 x( concentration,R~2= 0. 998); PE-1470 the background value is 14 ± 3( cpm),GC-1200 as the background value 57 ± 33( cpm); PE-1470 to~(125)I relative measurement efficiency is about 12% higher than GC-1200; PE-1470 and GC-1200 measurement precision is RSD = 0. 72% and RSD = 1. 42% respectively. Conclusion PE Wallac1470 WIZARD and Ustc Zon Kia GC-1200 γ counter were simple and convenient to operate,accuracy and sensitivity of PE-1470 were slightly better. Both are applicable to radioactive drug generation samples.
引文
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[2]管梁,朱承谨.国产放射免疫γ闪烁计数器及其临床应用[J].现代队学仪器与应用,1999,11(4):9-12.
[3]胡杰,汪太松,赵晋华.不同核素对GC-1500本底测定的影响[J].医疗卫生装备,2013,34(9):119-120.
[4]周杏琴,蔡刚明,钦晓峰,等.注射用重组人甲状旁腺素代谢产物PTH(1-34)的测定及其药动学[J].中国医院药学杂志,2012,32,(10):766-769.
[5]周杏琴,蔡刚明,钦晓峰,等.放射免疫分析法测定重组人甲状旁腺素(1-84)的血药浓度及其药动学研究[J].中国药房,2013,24(6):515-518.
[6]徐希杰,邹美芬,陈全成,等.2480 WIZARD2珈玛计数器测量正电子核素68 Ga的应用研究[J].中国辐射卫生,2013,22(6):651-653.
[7]石琴,贾福苏.谈谈临床放射免疫检验质量保证的几个问题[J].甘肃科技,2007,23(1):196-197.
[8]毛朝明,李龙.RIA及IRMA的质量保证[J].放射免疫学杂志,2002,15(6):361-363.
[9]裴忠亚,彭宪宝,孙涛.放射免疫实验的质量管理[J].齐鲁医学检验,2000,l1(4):1-2.
[10]任立平.评估放免试验效果的几点体会[J].中华医学研究杂志,2004,4(3):258-259.
[11]郭长河,尚文章,罗云杰.放射免疫试剂盒质量评价[J].当代医学,2009,l5(32):81-82.
[12]徐立根.放射免疫分析试剂盒质量控制[J].放射免疫杂志,2003,3:181-193.