气相色谱-质谱法检测体内、体外百草枯
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摘要
采用GC/MS测定体内、体外检材中百草枯。建立了测定血液、尿液、覆盆子果实枝叶以及土壤中百草枯的气相色谱质谱方法。不同样品经提取净化处理后,用GC-MS进行检测。血液、尿液、覆盆子枝叶、覆盆子果实、土壤中百草枯检出限分别为0.01μg/mL, 0.01μg/mL, 0.005μg/g, 0.005μg/g和0.008μg/g。
The paraquat in vivo and in vitro samples by GC/MS was studied. A gas chromatography mass spectrometry method was developed for the determination of paraquat in blood, urine, raspberry fruits, raspberry leaves and soil. The samples were extracted and purified, and then detected by GC-MS. The detection limit of paraquat was 0.01 μg/mL, 0.01 μg/mL, 0.005 μg/g,0.005 μg/g and 0.008 μg/g in blood, urine, raspberry leaves, raspberry fruits and soil, respectively.
The paraquat in vivo and in vitro samples by GC/MS was studied. A gas chromatography mass spectrometry method was developed for the determination of paraquat in blood, urine, raspberry fruits, raspberry leaves and soil. The samples were extracted and purified, and then detected by GC-MS. The detection limit of paraquat was 0.01 μg/mL, 0.01 μg/mL, 0.005 μg/g,0.005 μg/g and 0.008 μg/g in blood, urine, raspberry leaves, raspberry fruits and soil, respectively.
引文
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[15]孙秀兰,张银志,杨婷婷,等.食品中百草枯3种检测方法的比较[J].食品与生物技术学报,2010,29(6):883-888.
[2]刘文,张新威.毒品和毒物检验[M].北京:中国人民公安大学出版社,2003:596-599.
[3]沈敏,卓先义,廖林川,等.法医毒物司法鉴定实务[M].北京:法律出版社,2011:196-201.
[4]农业部种植业管理司.年中华人民共和国农业部公告第2445号[EB/OL].[2016-09-07].http://jiuban.moa.gov.cn/zwllm/tzgg/gg/201609/t20160913_5273423.htm.
[5]GAO L,LIU J,WANG C,et al.Fast determination of paraquat in plasma and urine samples bysolid-phase microextraction and gas chromatography-massspectrometry[J].Journal of Chromatography B,2014,944:136-140.
[6]黄璐瑶,廖林川,陈礼莉,等.硼氢化钠/氯化镍还原-GC/TSD法检测血液和尿液中百草枯[J].法医学杂志,2008,24(6):429-432.
[7]NORBERTO C POSECION,ENRIQUE M OSTREA,DAWN M BIELAWSKI,et al.Quantitative determination of paraquat in meconium by sodium borohydridenickel chloride chemical reduction and gas chrom-atography/mass spectrometry(GC/MS)[J].Journal of Chromatography B,2008,862:93-99.
[8]PEDRO NUNO MOREIRA,PAULA GUEDES DE PINBO,MARIA TERESA BALTAZAR,et al.Quantification of paraquat in postmortem samples by gas chromatographyion trap mass spectrometry and review of the literature[J].Biomed Chromatogr,2012,26(3):338-349.
[9]TAKESHI SAITO,TOMOKAZU FUKUSHIMA,KUKOYUI,et al.Monolithic spin column extraction and GC-MSfor the simultaneous assay of diquat,paraquat,and fenitrothion in human serum and urine[J].Anal Bioanal Chem,2011,400:25-31.
[10]李俊,王震,郭晓关,等.测定鱼腥草中百草枯和乙草胺残留的三重四级杆气相色谱质谱法[J].山地农业生物学报,2012,31(4):341-344.
[11]张向东,雷永良,李启,等.黑木耳中百草枯残留及其去除方法的研究和检测[J].中国卫生检验杂志,2017,27(16):2396-2398.
[12]潘碧枢,胡蒙燕.气相色谱-质谱联用仪检测血液中的百草枯[J].中国卫生检验杂志,2015,25(6):801-806.
[13]张婷,谭家锰,齐宝坤,等.气相色谱-串联质谱法分析尿和血中除草剂百草枯[J].中国法医学杂志,2009,24(3):161-163.
[14]张婷,马晓波.一种环境中百草枯残留含量的检测方法:CN 102608230 A[P].2012-07-25.
[15]孙秀兰,张银志,杨婷婷,等.食品中百草枯3种检测方法的比较[J].食品与生物技术学报,2010,29(6):883-888.