血吸虫病IHA筛查结果与疫情的相关性分析
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摘要
目的分析武汉市人群血吸虫病疫情与间接红细胞凝集试验(IHA)筛查结果之间的相关性,探讨IHA筛查结果在疫情评估中的应用价值。方法收集武汉市2007—2016年年报及监测点的数据,分析IHA阳性率与人群感染率之间相关性。结果武汉市2007—2016年血吸虫病人群感染率从0.58%降至0%,IHA阳性率从8.16%降至1.83%,人群感染率与IHA阳性率Pearson相关性r=0.845(P=0.002)。疫情控制、传播控制及传播阻断区IHA平均阳性率分别为5.12%、1.99%、0.26%,与人群感染率分布层次一致,存在等级相关关系(rs=0.836,P=0.000)。疫情控制区IHA阳性率范围为1.54%～8.79%,人群感染率范围为0.18%～0.71%;传播控制区IHA阳性率范围为0.14%～3.11%,人群感染率范围0～0.08%;传播阻断区IHA阳性率范围为0～0.57%,人群感染率均为0。监测点IHA阳性率由6.58%降至2.23%,感染率由0.76%降至0。2007—2016年血吸虫病人群感染率与IHA阳性率在武汉全市、疫情控制区、监测点水平上均存在良好的正相关关系(r>0.8,P<0.05)。结论人群感染率与IHA阳性率之间存在相关关系,IHA筛查结果能在一定程度上反映人群血吸虫感染水平,可作为血吸虫病疫情评价的指标之一。
Objective To explore the application value of of indirect hemagglutination assay( IHA) via analyzing thecorrelation between prevalence of schistosomiasis and positive rate by IHA in Wuhan. Methods Data of annual epidemic ofareas and surveillance sites were summarized in Wuhan from 2007 to 2016, and correlation between prevalence ofschistosomiasis and positive rate by IHA were analyzed. Results The infection rate of schistosomiasis in Wuhan was reducedfrom 0.58% to 0% while the positive rate of IHA was reduced from 8.16% to 1.83% from 2007 to 2016(r=0.845,P=0.002).The rank correlation coefficient of antibody positive rate(rs=0.836, P=0.000) was found between infection control, transmissioncontrol and transmission interruption, and the average positive rate of IHA was 5.12%, 1.99% and 0.26% respectively. Positiverate of IHA ranged from 1.54% to 8.79% while infection rates range 0.18%-0.71% in the infection control area. In thetransmission control area, positive rate of IHA ranged from 0.14% to 3.11% while infection rates range 0-0.08%. In thetransmission interruption area, positive rate ranged from 0 to 0.57% while infection rate was 0. In the IHA surveillance sites,positive rate decreased from 6.58% to 2.23%, and the infection rate decreased from 0.76% to 0. There was a positivecorrelation between positive incidence of schistosomiasis screening by IHA and infection rates of the population in Wuhan,infection control areas, surveillance sites(r>0.8,P<0.05). Conclusion There are strong correlation between positive rate byIHA and the schistosomiasis prevalence in population. It suggests that screening results of IHA can reflect the level ofschistosomiasis infection to some extent. During the extremely low detection rate by stool examination period, IHA can be triedas an indicator of schistosomiasis epidemic to spread more application.
Objective To explore the application value of of indirect hemagglutination assay( IHA) via analyzing thecorrelation between prevalence of schistosomiasis and positive rate by IHA in Wuhan. Methods Data of annual epidemic ofareas and surveillance sites were summarized in Wuhan from 2007 to 2016, and correlation between prevalence ofschistosomiasis and positive rate by IHA were analyzed. Results The infection rate of schistosomiasis in Wuhan was reducedfrom 0.58% to 0% while the positive rate of IHA was reduced from 8.16% to 1.83% from 2007 to 2016(r=0.845,P=0.002).The rank correlation coefficient of antibody positive rate(rs=0.836, P=0.000) was found between infection control, transmissioncontrol and transmission interruption, and the average positive rate of IHA was 5.12%, 1.99% and 0.26% respectively. Positiverate of IHA ranged from 1.54% to 8.79% while infection rates range 0.18%-0.71% in the infection control area. In thetransmission control area, positive rate of IHA ranged from 0.14% to 3.11% while infection rates range 0-0.08%. In thetransmission interruption area, positive rate ranged from 0 to 0.57% while infection rate was 0. In the IHA surveillance sites,positive rate decreased from 6.58% to 2.23%, and the infection rate decreased from 0.76% to 0. There was a positivecorrelation between positive incidence of schistosomiasis screening by IHA and infection rates of the population in Wuhan,infection control areas, surveillance sites(r>0.8,P<0.05). Conclusion There are strong correlation between positive rate byIHA and the schistosomiasis prevalence in population. It suggests that screening results of IHA can reflect the level ofschistosomiasis infection to some extent. During the extremely low detection rate by stool examination period, IHA can be triedas an indicator of schistosomiasis epidemic to spread more application.
引文
[1]李洋,罗华堂,周水茂,等. 2005-2014年武汉市血吸虫病监测点疫情分析[J].医学动物防制, 2016, 32(7):823-825,828.
[2]罗华堂,李洋,李群英,等. 2014年武汉市血吸虫病监测结果分析[J].热带病与寄生虫学, 2016, 14(1):35-37.
[3]朱蓉,秦志强,冯婷,等.全国血吸虫病监测点现场病原学检测效果及质控评估[J].中国血吸虫病防治杂志, 2013, 25(1):11-15.
[4]许静,陈年高,冯婷,等.日本血吸虫病常用诊断方法现场查病效果的评估[J].中国寄生虫学与寄生虫病杂志, 2007, 25(3):175-179.
[5]金嘉宁,党辉,张利娟,等. 2016年全国血吸虫病监测点疫情分析[J].中国寄生虫学与寄生虫病杂志, 2017, 35(6):542-548.
[6]张宁,冉瑞明,李文桂. Sj14-3-3在日本血吸虫病免疫诊断方面的研究进展[J].中国病原生物学杂志, 2013, 8(4):372-374.
[7]林丹丹,刘跃民,胡飞,等.日本血吸虫病常用诊断方法应用价值的评估ⅠIHA筛查法对血吸虫病疫区人群感染率的评价[J].中国血吸虫病防治杂志, 2008, 20(3):179-183.
[8]刘跃民,林丹丹,胡飞,等.日本血吸虫病常用诊断方法应用价值的评估ⅡIHA与Kato-Katz法结果的定量关系[J].中国血吸虫病防治杂志, 2010, 22(2):126-131.
[9]胡飞,李召军,李宜锋,等.血吸虫病不同流行程度流行区IHA法阳性诊断阈值的研究[J].中国血吸虫病防治杂志, 2016, 28(6):644-647,682.
[10]XU J, PEELING R W, CHEN J X, et al. Evaluation of immunoassays for the diagnosis of schistosoma japonicum infection using archived sera[J]. PLoS Neglected Tropical Diseases, 2011, 5(1):949.
[11]何家昶,张世清,汪天平,等. IHA筛查结果作为血吸虫病疫情判定指标应用价值探讨[J].热带病与寄生虫学, 2017, 15(2):96-98.
[12]长江日报.我市12个血吸虫疫区已提前一年实现目标.[EB/OL].[2018-02-27].http://www.wuhan.gov.cn/whszfwz/xwxx/whyw/201802/t20180227_187800.html.
[13]陈玲,江仁华,范宏萍. IHA与ELISA法在检测慢性日本血吸虫病中的应用比较[J].公共卫生与预防医学, 2012, 23(3):91-92.
[14]ZHOU X N, XU J, CHEN H G, et al. Tools to support policy decisions related to treatment strategies and surveillance of Schistosomiasis japonica towards elimination[J]. PLoS Negl Trop Dis, 2011, 5(12):1408.
[2]罗华堂,李洋,李群英,等. 2014年武汉市血吸虫病监测结果分析[J].热带病与寄生虫学, 2016, 14(1):35-37.
[3]朱蓉,秦志强,冯婷,等.全国血吸虫病监测点现场病原学检测效果及质控评估[J].中国血吸虫病防治杂志, 2013, 25(1):11-15.
[4]许静,陈年高,冯婷,等.日本血吸虫病常用诊断方法现场查病效果的评估[J].中国寄生虫学与寄生虫病杂志, 2007, 25(3):175-179.
[5]金嘉宁,党辉,张利娟,等. 2016年全国血吸虫病监测点疫情分析[J].中国寄生虫学与寄生虫病杂志, 2017, 35(6):542-548.
[6]张宁,冉瑞明,李文桂. Sj14-3-3在日本血吸虫病免疫诊断方面的研究进展[J].中国病原生物学杂志, 2013, 8(4):372-374.
[7]林丹丹,刘跃民,胡飞,等.日本血吸虫病常用诊断方法应用价值的评估ⅠIHA筛查法对血吸虫病疫区人群感染率的评价[J].中国血吸虫病防治杂志, 2008, 20(3):179-183.
[8]刘跃民,林丹丹,胡飞,等.日本血吸虫病常用诊断方法应用价值的评估ⅡIHA与Kato-Katz法结果的定量关系[J].中国血吸虫病防治杂志, 2010, 22(2):126-131.
[9]胡飞,李召军,李宜锋,等.血吸虫病不同流行程度流行区IHA法阳性诊断阈值的研究[J].中国血吸虫病防治杂志, 2016, 28(6):644-647,682.
[10]XU J, PEELING R W, CHEN J X, et al. Evaluation of immunoassays for the diagnosis of schistosoma japonicum infection using archived sera[J]. PLoS Neglected Tropical Diseases, 2011, 5(1):949.
[11]何家昶,张世清,汪天平,等. IHA筛查结果作为血吸虫病疫情判定指标应用价值探讨[J].热带病与寄生虫学, 2017, 15(2):96-98.
[12]长江日报.我市12个血吸虫疫区已提前一年实现目标.[EB/OL].[2018-02-27].http://www.wuhan.gov.cn/whszfwz/xwxx/whyw/201802/t20180227_187800.html.
[13]陈玲,江仁华,范宏萍. IHA与ELISA法在检测慢性日本血吸虫病中的应用比较[J].公共卫生与预防医学, 2012, 23(3):91-92.
[14]ZHOU X N, XU J, CHEN H G, et al. Tools to support policy decisions related to treatment strategies and surveillance of Schistosomiasis japonica towards elimination[J]. PLoS Negl Trop Dis, 2011, 5(12):1408.