香烟烟雾对雄性大鼠血清及睾丸组织中一氧化氮(NO)含量和一氧化氮合酶(iNOS)活性的影响
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摘要
目的探讨被动吸烟模型中香烟烟雾对雄性大鼠血清及睾丸组织中一氧化氮(NO)含量和一氧化氮合酶(i NOS)活性的影响。方法根据Haber理论建立大鼠被动吸烟模型,观察其体重变化、睾丸组织病理改变,并测定其血清及睾丸组织匀浆中NO含量和i NOS活性。结果对照组大鼠体重随时间的增加呈匀速增长状态(P <0. 05),实验组大鼠体重随染毒时间、剂量不断增加呈现缓慢增长状态,有的甚至出现负增长,高剂量组大鼠体重变化尤其明显(F=30. 24,P <0. 05);实验组大鼠睾丸组织出现明显病理病变,其血清和睾丸组织中NO含量明显大于对照组比值(P <0. 05),且i NOS活性均有改变且差异有统计学意义(F=6. 13,P <0. 05)。结论香烟烟雾对雄性大鼠血清及睾丸组织匀浆中NO含量和i NOS的活性均有影响,使其NO含量增加、i NOS活性发生改变,是大鼠睾丸组织出现损伤的可能机制之一。
Objective To study the effects of cigarette smoke on NO content and i NOS activity in serum and testis of male rats in passive smoking model. Methods The passive smoking model of rats was established according to Haber theory to observe the changes of body weight and pathological changes,and determine the content of NO and i NOS in serum and testis homogenate. Results The weight of rats in the control group increased regularly( P < 0. 05). The weight of the rats in the experimental group increased showed a slow growth with the increase of toxicity time and dose,and some even showed a negative growth. The weight change of rats in the high dose group was especially significant(( F = 30. 24,P < 0. 05). There were obvious pathological lesions in the testis of rats in the experimental group. The NO content in serum and testis tissues was significantly higher than that in the control group( P < 0. 05). The i NOS activity was changed and the difference was statistically significant( F = 6. 13,P <0. 05). Conclusion Cigarette smoke has an effect on the content of NO and the activity of i NOS in the serum and testis homogenate of male rats,which increases the content of NO and changes the activity of i NOS,which is one of the possible mechanisms leading to the damage of testicular tissue in rats.
Objective To study the effects of cigarette smoke on NO content and i NOS activity in serum and testis of male rats in passive smoking model. Methods The passive smoking model of rats was established according to Haber theory to observe the changes of body weight and pathological changes,and determine the content of NO and i NOS in serum and testis homogenate. Results The weight of rats in the control group increased regularly( P < 0. 05). The weight of the rats in the experimental group increased showed a slow growth with the increase of toxicity time and dose,and some even showed a negative growth. The weight change of rats in the high dose group was especially significant(( F = 30. 24,P < 0. 05). There were obvious pathological lesions in the testis of rats in the experimental group. The NO content in serum and testis tissues was significantly higher than that in the control group( P < 0. 05). The i NOS activity was changed and the difference was statistically significant( F = 6. 13,P <0. 05). Conclusion Cigarette smoke has an effect on the content of NO and the activity of i NOS in the serum and testis homogenate of male rats,which increases the content of NO and changes the activity of i NOS,which is one of the possible mechanisms leading to the damage of testicular tissue in rats.
引文
[1] FIALA J,HRUBA D,CRHA I,et al. Is environmental cadmium a serious hazard to Gzech population[J]. Int J Occup Med envir-on Health,2008,14(2):185-188.
[2] KOVAC JR,LABBATE C,RAMASAMY R,et al. Effects of cigarette smoking on erectile dysfunction[J]. Andrologia,2015,47(10):1087-1092.
[3]徐庆阳,祝茹.被动吸烟对大鼠睾丸和附睾抗氧化能力以及生精功能的影响[J].环境与健康杂志,2008,25(12):1059-1063.
[4]陈晓辉,戴静波,徐汪节,等.被动吸烟对雄性小鼠生殖能力的影响的研究[J].生殖与避孕,2014,34(10):805-808.
[5]古洪元,唐茂萍,方鹏,等.香烟烟雾对雄性小鼠生育能力的影响[J].上海交通大学学报(农业科学版),2012,30(3):1-5.
[6] XU WJ,FANG P,ZHU ZJ,et al. Cigarette smoking exposure alters pebpl DNA methylation and protein profile involved in MAPK signaling pathway in mice testis[J]. Biol Reprod,2013,89(6):142.
[7]张静,黄云飞,张晨,等.烟草烟雾致雄性大鼠睾丸损伤和血清IGF-1水平变化的影响[J].毒理学杂志,2016,30(4):274-276.
[8]仲春雪,张静,何丽娟,等.香烟烟雾对大鼠睾丸NO含量及Na+-K+-ATP酶、CA2+-ATP酶活性的影响活[J].毒理学杂志,2016,30(1):6-9.
[9] ZHU ZJ,XU WJ,DAI JB,et al. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis[J].Inter J Biochem Cell Biol,2013,45(3):571-582.
[10] POLSKY JY,ARONSON KJ,HEATON JP,et al. Smoking and other lifestyle factors in relation to erectile dysfunction[J]. BJU International,2005,96(9):1355-1359.
[11] TASZAREK HG,DEPA MARTYNOW M,DERWICH K,et al. The influence of cigarette smoking on sperm quality of male smokers and nommokers in infertile couples[J]. Przegl Lek,2005,62(10):978-981.
[12]陆金春.一氧化氮合成酶和一氧化氮对生殖活动的作用[J].生殖与避孕,1996,16(3):167-168.
[13]任亚萍,孙莉,毛庭枝,等. i NOS在大鼠性成熟期睾丸组织的表达变化及意义[J].华夏医学,2009,22(5):841-842.
[14]孙莉,任亚萍,江伟,等.一氧化氮合酶在大鼠睾丸及附睾中的表达和定位[J].广西医科大学学报,2014,21(4):506-507.
[15] SORTINO MA,ALEPPO G,SEAPAGNINI U,et al. Involvement of nitrio oxide in the regulation of gonadotropin-releasing hormone teleasse from the GT-1 neuronal cell line[J]. Endocrinology,1994,134(4):1782-1782.
[16] BERDEAUX A. Nitric oxide:an ubiquitous messenger[J]. Fundam Clin Pharmacol,1993,7(8):401-411.
[17] MONCADA S,PALMER RM,HIGGS EA. Nitric oxide:physiology,pathophysiology,and pharmacology[J]. Pharmacol Rev,1991,43(2):109-142.
[18] CORBUCCI GG,LETTIERI B,DAMONTI V,et al. Nitric oxide in ischemic and reperfused human muscle[J]. Clin Chim Acta,2002,318(1/2):79-82.
[19] SNYDER SH,JAFFREY SR,ZAKHARY R. Nitric oxide and carbon monoxide:parallel roles as neural messengers[J]. Brain Res Brain Res Rev,1998,26(2/3):167-175.
[20] BURNETT AL,LOWENSTEIN CJ,BREDT DS,et al. Nitric oxide:A physiologic mediator of penile erection[J]. Science,1992,257(5068):401-403.
[21]杨最素,朱有法,俞建军.诱导型一氧化氮合酶在精索静脉曲张大鼠睾丸中的表达[J].解剖学杂志,2006,29(6):728-733.
[22] ZHANG H,ZHENG RL. Possible role of nitric oxide on fertile and asthenozoospermic infertile human sperm functlions[J]. Free Radic Res,1996,25(4):347-354.
[23]邓菁,陈国元,季佳佳,等.二硫化碳对大鼠睾丸组织氧自由基及抗氧化水平的影响[J].华中科技大学学报(医学版),2006,35(2):228-230.
[24]王世鄂,陈奕权,周瑞祥.一氧化氮合酶同功异构酶在大鼠睾丸中的表达和定位[J].生殖医学杂志,2001,10(2):99-102.
[25]田溪泉,潘天明,郭应禄.一氧化氮对精子运动功能的影响[J].中华泌尿外科杂志,1999,20(8):496-498.
[26]秦文波,王淑秋,王跃生.氧自由基与精索静脉曲张不育关系的研究[J].中华泌尿外科杂志,1996,17(11):689-691.
[27] AKSOY H,AKSOY Y,OZKEY I,et al. The relationship between varicocele and semen nitric oxide[J]. Urol Res,2000,28(6):357-359.
[28] UCKERT S,SCHELLER F,STIEF CG,et al. Potential mechanism of action of human growth hormone on isolated human penile erectile tissue[J]. Urology,2010,75(4):968-973.
[2] KOVAC JR,LABBATE C,RAMASAMY R,et al. Effects of cigarette smoking on erectile dysfunction[J]. Andrologia,2015,47(10):1087-1092.
[3]徐庆阳,祝茹.被动吸烟对大鼠睾丸和附睾抗氧化能力以及生精功能的影响[J].环境与健康杂志,2008,25(12):1059-1063.
[4]陈晓辉,戴静波,徐汪节,等.被动吸烟对雄性小鼠生殖能力的影响的研究[J].生殖与避孕,2014,34(10):805-808.
[5]古洪元,唐茂萍,方鹏,等.香烟烟雾对雄性小鼠生育能力的影响[J].上海交通大学学报(农业科学版),2012,30(3):1-5.
[6] XU WJ,FANG P,ZHU ZJ,et al. Cigarette smoking exposure alters pebpl DNA methylation and protein profile involved in MAPK signaling pathway in mice testis[J]. Biol Reprod,2013,89(6):142.
[7]张静,黄云飞,张晨,等.烟草烟雾致雄性大鼠睾丸损伤和血清IGF-1水平变化的影响[J].毒理学杂志,2016,30(4):274-276.
[8]仲春雪,张静,何丽娟,等.香烟烟雾对大鼠睾丸NO含量及Na+-K+-ATP酶、CA2+-ATP酶活性的影响活[J].毒理学杂志,2016,30(1):6-9.
[9] ZHU ZJ,XU WJ,DAI JB,et al. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis[J].Inter J Biochem Cell Biol,2013,45(3):571-582.
[10] POLSKY JY,ARONSON KJ,HEATON JP,et al. Smoking and other lifestyle factors in relation to erectile dysfunction[J]. BJU International,2005,96(9):1355-1359.
[11] TASZAREK HG,DEPA MARTYNOW M,DERWICH K,et al. The influence of cigarette smoking on sperm quality of male smokers and nommokers in infertile couples[J]. Przegl Lek,2005,62(10):978-981.
[12]陆金春.一氧化氮合成酶和一氧化氮对生殖活动的作用[J].生殖与避孕,1996,16(3):167-168.
[13]任亚萍,孙莉,毛庭枝,等. i NOS在大鼠性成熟期睾丸组织的表达变化及意义[J].华夏医学,2009,22(5):841-842.
[14]孙莉,任亚萍,江伟,等.一氧化氮合酶在大鼠睾丸及附睾中的表达和定位[J].广西医科大学学报,2014,21(4):506-507.
[15] SORTINO MA,ALEPPO G,SEAPAGNINI U,et al. Involvement of nitrio oxide in the regulation of gonadotropin-releasing hormone teleasse from the GT-1 neuronal cell line[J]. Endocrinology,1994,134(4):1782-1782.
[16] BERDEAUX A. Nitric oxide:an ubiquitous messenger[J]. Fundam Clin Pharmacol,1993,7(8):401-411.
[17] MONCADA S,PALMER RM,HIGGS EA. Nitric oxide:physiology,pathophysiology,and pharmacology[J]. Pharmacol Rev,1991,43(2):109-142.
[18] CORBUCCI GG,LETTIERI B,DAMONTI V,et al. Nitric oxide in ischemic and reperfused human muscle[J]. Clin Chim Acta,2002,318(1/2):79-82.
[19] SNYDER SH,JAFFREY SR,ZAKHARY R. Nitric oxide and carbon monoxide:parallel roles as neural messengers[J]. Brain Res Brain Res Rev,1998,26(2/3):167-175.
[20] BURNETT AL,LOWENSTEIN CJ,BREDT DS,et al. Nitric oxide:A physiologic mediator of penile erection[J]. Science,1992,257(5068):401-403.
[21]杨最素,朱有法,俞建军.诱导型一氧化氮合酶在精索静脉曲张大鼠睾丸中的表达[J].解剖学杂志,2006,29(6):728-733.
[22] ZHANG H,ZHENG RL. Possible role of nitric oxide on fertile and asthenozoospermic infertile human sperm functlions[J]. Free Radic Res,1996,25(4):347-354.
[23]邓菁,陈国元,季佳佳,等.二硫化碳对大鼠睾丸组织氧自由基及抗氧化水平的影响[J].华中科技大学学报(医学版),2006,35(2):228-230.
[24]王世鄂,陈奕权,周瑞祥.一氧化氮合酶同功异构酶在大鼠睾丸中的表达和定位[J].生殖医学杂志,2001,10(2):99-102.
[25]田溪泉,潘天明,郭应禄.一氧化氮对精子运动功能的影响[J].中华泌尿外科杂志,1999,20(8):496-498.
[26]秦文波,王淑秋,王跃生.氧自由基与精索静脉曲张不育关系的研究[J].中华泌尿外科杂志,1996,17(11):689-691.
[27] AKSOY H,AKSOY Y,OZKEY I,et al. The relationship between varicocele and semen nitric oxide[J]. Urol Res,2000,28(6):357-359.
[28] UCKERT S,SCHELLER F,STIEF CG,et al. Potential mechanism of action of human growth hormone on isolated human penile erectile tissue[J]. Urology,2010,75(4):968-973.