人面果叶总黄酮的提取工艺研究
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摘要
目的:优化人面果叶总黄酮的提取工艺.方法:利用单因素和正交实验法,以干膏得率和总黄酮得率为评价指标,考察了乙醇浓度、料液比和提取温度等因素对提取效果的影响.结果:乙醇浓度70%,提取温度80℃,料液比1:6,提取时间0.5 h,为人面果叶总黄酮的最优提取参数.按该工艺提取次数1次,总黄酮得率可达4.96%.结论:该工艺优化了人面果叶总黄酮的提取过程,并验证了其重现性.
Objective: To optimize the extraction of total flavonoids from the leaves of Garcinia xanthochymus. Methods: Single-factor and orthogonal test were employed by using the yields of dry extract and the total flavonoids as evaluating indices, and the effects of ethanol concentration, solid-liquid ratio and extraction temperature were investigated. Results: The optimal extraction parameters of total flavonoids was determined as follows: ethanol concentration 70%, extraction temperature 80 ℃, solid-liquid ratio 1:6, extracting time 0.5 h. The total flavonoid yield could reach 4.96% with one extraction under such condition. Conclusion: The extraction of total flavonoids from the leaves of Garcinia xanthochymus was optimized, and the reproducibility of the process was verified.
Objective: To optimize the extraction of total flavonoids from the leaves of Garcinia xanthochymus. Methods: Single-factor and orthogonal test were employed by using the yields of dry extract and the total flavonoids as evaluating indices, and the effects of ethanol concentration, solid-liquid ratio and extraction temperature were investigated. Results: The optimal extraction parameters of total flavonoids was determined as follows: ethanol concentration 70%, extraction temperature 80 ℃, solid-liquid ratio 1:6, extracting time 0.5 h. The total flavonoid yield could reach 4.96% with one extraction under such condition. Conclusion: The extraction of total flavonoids from the leaves of Garcinia xanthochymus was optimized, and the reproducibility of the process was verified.
引文
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[2] 林艳芳,依专,赵应红.中国傣医药彩色图谱[M].云南: 云南民族出版社,2003: 6.
[3] PERRY L M,METZGER J.Medicinal plants of east and southeast Asia: attributed properties and uses [M].Cambridge: MIT Press,1980.
[4] AMBASTA S P.The useful plants of India[M].New Delhi: Council of Scientific and Industrial Research publications,1986: 231.
[5] 付蒙,胡鑫,徐婧,等.人面果叶、根部、果实提取物体外抗糖尿病活性成分研究[J].天然产物研究与开发,2014,26 (2): 255-259.
[6] 刘流,李芸芳,甘飞,等.人面果叶子的化学成分研究[J].中国中药杂志,2016,41 (11): 2098-2104.
[7] LI Y,CHEN Y,XIAO C,et al.Rapid screening andidenti?-cation of α-amylase inhibitors from Garcinia xanthochymus using enzyme-immobilized magnetic nanoparticles coupled with HPLC and MS [J].J Chromatogr B,2014,960: 166-173.
[8] LI Y F,XU J,CHEN Y,et al.Screening of inhibitors of glycogen synthase kinase-3β from traditional Chinese medicines using enzyme-immobilized magnetic beads combined with high-performance liquid chromatography[J].J Chromatogr A,2015,1425: 8-16.
[9] 刘虹,肖敏,覃瑞,等.皱皮木瓜总酚酸提取工艺研究[J].中南民族大学学报(自然科学版),2017,36(2): 49-52.
[10] 王少兵,田兵,操冰冰,等.龙血竭总黄酮提取工艺的优化[J].中南民族大学学报(自然科学版),2015,34(4): 58-61.