Cys LTR-1和NEDD9在胰腺癌中的表达及与临床病理参数的关系
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摘要
目的分析半胱氨酰白三烯受体1(Cys LTR-1)、神经前体细胞表达下调蛋白9(NEDD9)在胰腺癌和癌旁组织中的表达情况,及其与胰腺癌的发展关系。方法回顾性收集2015年1月至2016年10月行手术治疗的86例胰腺癌患者的癌和癌旁组织样本,分别检测样本中Cys LTR-1 mRNA和NEDD9,并对Cys LTR-1 mRNA、NEDD9的表达情况与临床病理特征的关系进行分析。结果 Cys LTR-1 mRNA和NEDD9在癌组织中的表达均显著高于癌旁组织,差异具有统计学意义(P<0.05);且胰腺癌组织中Cys LTR-1 mRNA及NEDD9的表达情况与患者肿瘤分化程度、TNM分期和淋巴结转移相关,差异具有统计学意义(P<0.05)。结论 Cys LTR-1 mRNA和NEDD9在胰腺癌组织中呈高表达,可能与胰腺癌侵袭、浸润等有关,可作为判断胰腺癌预后的分子标志物。
Objective To analyze the expression of Cys LTR-1 and NEDD9 in pancreatic cancer and para-carcinoma tissue,and the development of pancreatic cancer. Methods Samples of pancreatic cancer tissue and para-carcinoma tissue of 86 patients with pancreatic cancer from January 2015 to October 2016 were collected in our hospital surgical treatment,Cys LTR-1 mRNA and NEDD9 were tested,the relationship between the expression of Cys LTR-1 mRNA and NEDD9 and clinicopathological features was analyzed. Results The expression of Cys LTR-1 mRNA and NEDD9 in pancreatic cancer tissue were significantly higher than those of the para-carcinoma tissue,and the difference was statistically significant( P < 0. 05). The expression of Cys LTR-1 mRNA and NEDD9 in pancreatic cancer tissue was associated with TNM stage,differentiated degree and lymph node metastasis( P < 0. 05). Conclusion Cys LTR-1 mRNA and NEDD9 are highly expressed in pancreatic cancer tissue and may be associated with pancreatic cancer infiltration and invasion,can be used as a molecular marker for the prognosis of pancreatic cancer.
Objective To analyze the expression of Cys LTR-1 and NEDD9 in pancreatic cancer and para-carcinoma tissue,and the development of pancreatic cancer. Methods Samples of pancreatic cancer tissue and para-carcinoma tissue of 86 patients with pancreatic cancer from January 2015 to October 2016 were collected in our hospital surgical treatment,Cys LTR-1 mRNA and NEDD9 were tested,the relationship between the expression of Cys LTR-1 mRNA and NEDD9 and clinicopathological features was analyzed. Results The expression of Cys LTR-1 mRNA and NEDD9 in pancreatic cancer tissue were significantly higher than those of the para-carcinoma tissue,and the difference was statistically significant( P < 0. 05). The expression of Cys LTR-1 mRNA and NEDD9 in pancreatic cancer tissue was associated with TNM stage,differentiated degree and lymph node metastasis( P < 0. 05). Conclusion Cys LTR-1 mRNA and NEDD9 are highly expressed in pancreatic cancer tissue and may be associated with pancreatic cancer infiltration and invasion,can be used as a molecular marker for the prognosis of pancreatic cancer.
引文
[1]丁雪,李晓梅.协同刺激分子B7-H4在消化系统肿瘤中的研究进展[J].中国肿瘤,2016,25(9):706-710.
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[11]Ding X,Zhu C,Qiang H,et al.Enhancing antitumor effects in pancreatic cancer cells by combined use of COX-2 and 5-LOX inhibitors[J].Biomed Pharmacother,2011,65(7):486-490.
[12]吴铁龙,薛丽莉,戴圆圆,等.神经前体细胞表达下调蛋白9在胰腺癌组织中的表达及临床意义[J].临床肝胆病杂志,2017,33(1):130-132.
[13]Kozyulina PY,Loskutov YV,Kozyreva VK,et al.Prometastatic NEDD9 Regulates Individual Cell Migration via Caveolin-1-Dependent Trafficking of Integrins[J].Mol Cancer Res,2015,13(3):423-438.
[14]Zhang SS,Wu LH,Liu Q,et al.Elevated expression of NEDD9 is associated with metastatic activity in gastric cancer[J].Onco Targets Ther,2015,11(8):633-640.
[15]卢鹏.NEDD9在肝癌中的表达及其与肝癌细胞生物学特性之间关系的研究[D].西安:第四军医大学,2015.
[16]Sima N,Cheng X,Ye F,et al.The overexpression of scaffolding protein NEDD9 promotes migration and invasion in cervical cancer via tyrosine phosphorylated FAK and SRC[J].PLo S One,2013,8(9):e74594.
[17]徐松辉.ACK1基因扩增调控胃癌发生和转移的机制及其与临床预后关系的研究[D].广州:暨南大学,2016.
[18]田静,产翠翠,方蕾,等.抑癌基因LKB1在肺癌中的研究进展[J].辽宁医学院学报,2016,37(4):104-107.
[19]Feng J,Zhao J,Xie H,et al.Involvement of NEDD9 in the invasion and migration of gastric cancer[J].Tumour Biol,2015,36(5):3621-3628.
[2]Luo J,Xiao L,Wu C,et al.The incidence and survival rate of population-based pancreatic cancer patients:Shanghai CancerRegistry 2004-2009[J].PLo S One,2013,8(10):e76052.
[3]吕海锋,马炳奇,张顺,等.胰腺癌组织Stat3、HIF-1α与VEGF表达及意义[J].齐鲁医学杂志,2014,12(3):196-198.
[4]孟艳,朱莹,张亚庆,等.半胱氨酰白三烯受体1在胰腺癌组织中的表达及临床意义[J].临床肝胆病杂志,2017,33(1):133-136.
[5]张文,李少军,赵云龙,等.敲低神经前体细胞表达的发育性下调基因9(NEDD9)抑制EC109食管癌细胞的增殖、侵袭和迁移[J].细胞与分子免疫学杂志,2016,32(12):1662-1666.
[6]中华医学会外科学分会胰腺外科学组.胰腺癌诊治指南(2014)[J].临床肝胆病杂志,2014,30(12):1240-1245.
[7]李斌.晚期胰腺癌患者应用吉西他滨联合替吉奥治疗的临床效果评价[J].临床和实验医学杂志,2016,15(8):771-773.
[8]Hennig R,Ding XZ,Tong WG,et al.5-Lipoxygenase and leukotriene B(4)receptor are expressed in human pancreatic cancers but not in pancreatic ducts in normal tissue[J].Am J Pathol,2002,161(2):421-428.
[9]张世强,唐采白,陈卫花.半胱氨酰白三烯受体Cys LT1R在大肠癌组织中的表达及临床意义[J].山西医科大学学报,2014,45(3):177-179.
[10]刘伟.Cys LT1和Cys LT2受体与乳腺癌相关性的研究[D].天津:天津医科大学,2011.
[11]Ding X,Zhu C,Qiang H,et al.Enhancing antitumor effects in pancreatic cancer cells by combined use of COX-2 and 5-LOX inhibitors[J].Biomed Pharmacother,2011,65(7):486-490.
[12]吴铁龙,薛丽莉,戴圆圆,等.神经前体细胞表达下调蛋白9在胰腺癌组织中的表达及临床意义[J].临床肝胆病杂志,2017,33(1):130-132.
[13]Kozyulina PY,Loskutov YV,Kozyreva VK,et al.Prometastatic NEDD9 Regulates Individual Cell Migration via Caveolin-1-Dependent Trafficking of Integrins[J].Mol Cancer Res,2015,13(3):423-438.
[14]Zhang SS,Wu LH,Liu Q,et al.Elevated expression of NEDD9 is associated with metastatic activity in gastric cancer[J].Onco Targets Ther,2015,11(8):633-640.
[15]卢鹏.NEDD9在肝癌中的表达及其与肝癌细胞生物学特性之间关系的研究[D].西安:第四军医大学,2015.
[16]Sima N,Cheng X,Ye F,et al.The overexpression of scaffolding protein NEDD9 promotes migration and invasion in cervical cancer via tyrosine phosphorylated FAK and SRC[J].PLo S One,2013,8(9):e74594.
[17]徐松辉.ACK1基因扩增调控胃癌发生和转移的机制及其与临床预后关系的研究[D].广州:暨南大学,2016.
[18]田静,产翠翠,方蕾,等.抑癌基因LKB1在肺癌中的研究进展[J].辽宁医学院学报,2016,37(4):104-107.
[19]Feng J,Zhao J,Xie H,et al.Involvement of NEDD9 in the invasion and migration of gastric cancer[J].Tumour Biol,2015,36(5):3621-3628.