摘要
为检测成年雌兔背根神经节中是否存在雌激素受体(ER)与M2型乙酰胆碱受体(M2mAchR)的分布与共存,采用免疫荧光双标记染色法对ER与M2mAchR在背根神经节中的分布情况进行了研究。结果显示,在成年雌兔背根神经节中:(1) ER分布主要以神经元为主。其中,ERα主要表达于中小型神经元,而ERβ则多分布于大中型神经元。(2)相较ER而言,M2mAchR分布更为广泛,除神经元外,神经纤维等组织上也有弱阳性表达。(3)两种ER与M2mAchR均有共存现象,但共存情况存在差异,ERα/M2mAchR主要共分布于中小型细胞,ERβ/M2mAchR主要共分布于大中型神经元。结果表明,首先,雌激素具有通过影响背根神经节部分神经元的活动而实现对躯体内脏感觉活动间接调节的条件。其次,在此影响的机制中,雌激素除可以直接作用于神经元上的受体之外,还具有与共表达的M2mAchR交互作用而实现的条件。为研究雌激素影响神经系统调控活动的机制以及背根神经节的神经内分泌调节机制提供了重要的形态学依据。
Double immunofluorescence staining method were used to detect the distribution and co-expression of estrogen receptor(ER) and M2-acetylcholine receptor(M2 mAchR) in the dorsal root ganglion(DRG) of female adult rabbits.The results of immunofluorescence staining in DRG show that,firstly,ER were distributed in nerve cells mainly.Among them,small and medium-sized neurons were strong or medium positive to ERα.However,the cells with strong positive to ERβ,were observed in large and medium-sized neurons mostly,secondly,M2 mAchR immunopositivity was more widely distributtion than ER.Besides neurons,there were also weakly positive to others such as nerve fibers,finally,both types of ER and M2 mAchR were co-expressed in DRG.But the condition of these co-expressions were differenced,ERα importantly located in small and medium-sized neurons,and ERβ mostly in large and medium-sized neurons.These results suggested that estrogen may regulate the somatic and visceral sensory activity by affecting dorsal root ganglion neurons;in addition to immediately affect the ER in neurons,estrogen can also be achieved this role by acting on co-expression of M2 mAchR and ER.This result provides a morphological basis for studying the role of estrogen in regulating activity of nervous system through multiple pathways,and the neuroendocrine regulation mechanism of DRG.
引文
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