岩藻多糖结构对其功能的影响研究进展
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摘要
岩藻多糖是一种富含硫酸基的多聚糖,广泛存在于褐色海藻中。由于其广泛的生物活性功能,如抗氧化、提升免疫力以及抑制肿瘤等,从而逐渐成为目前研究的热点。天然来源的岩藻多糖由于分子聚合度较高,从而限制了其在相关领域的应用。随着研究的深入,岩藻多糖的结构以及不同来源的岩藻多糖对其生物活性的影响成为新的研究方向。本文重点介绍了岩藻多糖结构对其抗氧化活性、抑菌性、提高免疫力以及抗肿瘤活性的影响,并分析了目前岩藻多糖研究存在的挑战,为后续岩藻多糖潜在的商业化应用和科学研究提供思路。
Fucoidin is a kind of polysaccharide rich in sulfuric,which widely found in brown algae. Due to its extensive biological activities,such as anti-oxidation,immunity enhancement and anticancer,it has gradually become the focus of current research. The molecular polymerization of natural fucoidan was in high degree,which limits its application in related fields. With the deepening of research,the effect of structure and the different sources of fucoidin on their bioactivity have become a new research direction. In our study,the effect of the structure of fucoidan on its biological activity,which is anti-oxidation,anti-bacteria,immunity enhancement and tumor suppression were highlighted. In addition,the challenges of fucoidan research were also analyzed,which provides idears for the fucoidan application and science research.
Fucoidin is a kind of polysaccharide rich in sulfuric,which widely found in brown algae. Due to its extensive biological activities,such as anti-oxidation,immunity enhancement and anticancer,it has gradually become the focus of current research. The molecular polymerization of natural fucoidan was in high degree,which limits its application in related fields. With the deepening of research,the effect of structure and the different sources of fucoidin on their bioactivity have become a new research direction. In our study,the effect of the structure of fucoidan on its biological activity,which is anti-oxidation,anti-bacteria,immunity enhancement and tumor suppression were highlighted. In addition,the challenges of fucoidan research were also analyzed,which provides idears for the fucoidan application and science research.
引文
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[17]Li G Y,Luo Z C,Yuan F,et al.Combined process of high-pressure homogenization and hydrothermal extraction for the extraction of fucoidan with good antioxidant properties from Nemacystus decipients[J].Food & Bioproducts Processing,2017,106:35-42.
[18]Choi J I,Lee S G,Han S J,et al.Effect of gamma irradiation on the structure of fucoidan[J].Radiation Physics & Chemistry,2014,100(1):54-58.
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[20]Mc R D S,Marques C T,Guerra Dore C M,et al.Antioxidant activities of sulfated polysaccharides from brown and red seaweeds[J].Journal of Applied Phycology,2007,19(2):153-160.
[21]Lahrsen E,Liewert I,Alban S.Gradual degradation of fucoidan from Fucus vesiculosus,and its effect on structure,antioxidant and antiproliferative activities[J].Carbohydrate Polymers,2018,192:208-216.
[22]Vo T S,Kim S K.Fucoidans as a natural bioactive ingredient for functional foods[J].Journal of Functional Foods,2013,5(1):16-27.
[23]张加幸,苗钧魁,冷凯良.收获期内不同品种鲜海带中岩藻多糖含量、组成及其抗氧化活性研究[J].应用化工,2016,45(11):2186-2189.
[24]Marudhupandi T.Antibacterial effect of fucoidan from Sargassum wightii against the chosen human bacterial pathogens[J].International Current Pharmaceutical Journal,2013,2(10):156-158.
[25]Liu M,Liu Y,Cao M J,et al.Antibacterial activity and mechanisms of depolymerized fucoidans isolated from Laminaria japonica[J].Carbohydrate Polymers,2017,172:294-305.
[26]Feng H,Ying Y,Yang G A,et al.Studies on antibacterial activity and antibacterial mechanism of a novel polysaccharide from Streptomyces virginia H03[J].Food Control,2010,21(9):1257-1262.
[27]Yamashita S,Sugita-Konishi Y,Shimizu M.In vitro bacteriostatic effects on dietary polysaccharides[J].Food Science & Technology International Tokyo,2001,7(3):262-264.
[28]Hwang P A,Hung Y L,Phan N N,et al.The in vitro and in vivo effects of the low molecular weight fucoidan on the bone osteogenic differentiation properties[J].Cytotechnology,2016,68(4):1349-1359.
[29]石磊,高欣,赵艳威,等.岩藻多糖对小鼠免疫功能的影响[J].食品研究与开发,2012,33(12):194-196.
[30]Yuguchi Y,Tran V T,Bui L M,et al.Primary structure,conformation in aqueous solution,and intestinal immunomodulating activity of fucoidan from two brown seaweed species Sargassum crassifolium and Padina australis[J].Carbohydrate Polymers,2016,147:69-78.
[31]Khilaa Chenko S,Zaporozhets T,Shevchenko N,et al.Immunostimulatory activity of fucoidan from the brown alga Fucus evanescens:Role of sulfates and acetates[J].Journal of Carbohydrate Chemistry,2011,30(4-6):291-305.
[32]Vishchuk O S,Ermakova S P,Zvyagintseva T N.The fucoidans from brown algae of Far-Eastern seas:Anti-tumor activity and structure-function relationship[J].Food Chemistry,2013,141(2):1211-1217.
[33]Usoltseva R V,Anastyuk S D,Ishina I A,et al.Structural characteristics and anticancer activity in vitro,of fucoidan from brown algae Padina boryana[J].Carbohydrate Polymers,2018,184:260-268.
[34]Silchenko A S,Rasin A B,Kusaykin M I,et al.Modification of native fucoidan from Fucus evanescens by recombinant fucoidanase from marine bacteria Formosa algae[J].Carbohydrate Polymers,2018,193:189-195.
[35]Vo T S,Kim S K.Fucoidans as a natural bioactive ingredient for functional foods[J].Journal of Functional Foods,2013,5(1):16-27.
[36]Chandía N P,Matsuhiro B.Characterization of a fucoidan from Lessonia vadosa(Phaeophyta)and its anticoagulant and elicitor properties[J].International Journal of Biological Macromolecules,2008,42(3):235-240.
[37]Nishino T,Aizu Y,Nagumo T.The influence of sulfate content and molecular weight of a fucan sulfate from the brown seaweed Ecklonia kurome on its antithrombin activity[J].Thrombosis Research,1991,64(6):723-731.
[38]Park H Y,Han M H,Park C,et al.Anti-inflammatory effects of fucoidan through inhibition of NF-B,MAPK and Akt activation in lipopolysaccharide-induced BV2 microglia cells[J].Food and Chemical Toxicology,2011,49,1745-1752.
[39]Park S B,Chun K R,Kim J K,et al.The differential effect of high and low molecular weight fucoidans on the severity of collagen-induced arthritis in mice[J].Phytotherapy Research,2010,24(9):1384-1391.
[40]Jiao G,Yu G,Zhang J,et al.Chemical structures and bioactivities of sulfated polysaccharides from Marine algae[J].Marine Drugs,2011,9(2):196-223.
[41]Sinha S,Astani A,Ghosh T,et al.Polysaccharides from Sargassum tenerrimum:Structural features,chemical modification and anti-viral activity[J].Phytochemistry,2010,71(2-3):235-242.
[42]Mandal P,Mateu C G,Chattopadhyay K,et al.Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica[J].Antiviral Chemistry & Chemotherapy,2007,18(3):153-162.
[2]史大华,刘玮炜,刘永江,等.低分子量海带岩藻多糖的制备及其抗肿瘤活性研究[J].时珍国医国药,2012,23(1):53-55.
[3]Duarte M E,Cardoso M A,Noseda M D,et al.Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum[J].Carbohydr Res,2001,333(4):281-293.
[4]Jin J,Zhang W,Du J Y,et al.Fucoidan can function as an adjuvant in vivo to enhance dendritic cell maturation and function and promote antigen-specific T cell immune responses[J].Plos One,2014,9(6):e99396.
[5]Teruya T,Konishi T,Uechi S,et al.Anti-proliferative activity of oversulfated fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA in U937 cells[J].International Journal of Biological Macromolecules,2007,41(3):221-226.
[6]Athukorala Y,Jung W K,Vasanthan T,et al.An anticoagulative polysaccharide from an enzymatic hydrolysate of Ecklonia cava[J].Carbohydrate Polymers,2006,66(2):184-191.
[7]Ferreira S S,Passos C P,Madureira P,et al.Structure-function relationships of immunostimulatory polysaccharides:A review.[J].Carbohydr Polym,2015,132:378-396.
[8]Chen S,Hu Y,Ye X,et al.Sequence determination and anticoagulant and antithrombotic activities of a novel sulfated fucan isolated from the sea cucumber Isostichopus badionotus[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2012,1820(7):989-1000.
[9]Yu L,Xue C,Chang Y,et al.Structure and rheological characteristics of fucoidan from sea cucumber Apostichopus japonicus[J].Food Chemistry,2015,180:71-76.
[10]Hemmingson J A,Falshaw R,Furneaux R H,et al.Structure and antiviral activity of the galactofucan sulfates extracted from Undaria,Pinnatifida,(Phaeophyta)[J].Journal of Applied Phycology,2006,18(2):185.
[11]Wu L,Sun J,Su X,et al.A review about the development of fucoidan in antitumor activity:Progress and challenges[J].Carbohydrate Polymers,2016,154:96-111.
[12]王芸,张淑平.岩藻多糖制备及结构研究进展[J].应用化工,2015,44(1):146-149.
[13]Choi J I,Kim H J.Preparation of low molecular weight fucoidan by gamma-irradiation and its anticancer activity[J].Carbohydrate Polymers,2013,97(2):358-362.
[14]Holtkamp A,Kelly S,Ulber R,et al.Fucoidans and fucoidanases-focus on techniques for molecular structure elucidation and modification of marine polysaccharides[J].Applied Microbiology & Biotechnology,2009,82(1):1-11.
[15]Morya V K,Kim J,Kim E K.Algal fucoidan:Structural and size-dependent bioactivities and their perspectives[J].Applied Microbiology & Biotechnology,2012,93(1):71-82.
[16]Guo X,Ye X,Sun Y,et al.Ultrasound effects on the degradation kinetics,structure,and antioxidant activity of sea cucumber fucoidan[J].Journal of Agricultural & Food Chemistry,2014,62(5):1088-1095.
[17]Li G Y,Luo Z C,Yuan F,et al.Combined process of high-pressure homogenization and hydrothermal extraction for the extraction of fucoidan with good antioxidant properties from Nemacystus decipients[J].Food & Bioproducts Processing,2017,106:35-42.
[18]Choi J I,Lee S G,Han S J,et al.Effect of gamma irradiation on the structure of fucoidan[J].Radiation Physics & Chemistry,2014,100(1):54-58.
[19]Changotade S I,Korb G,Bassil J,et al.Potential effects of a low-molecular-weight fucoidan extracted from brown algae on bone biomaterial osteoconductive properties[J].Journal of Biomedical Materials Research Part A,2008,87A(3):666-675.
[20]Mc R D S,Marques C T,Guerra Dore C M,et al.Antioxidant activities of sulfated polysaccharides from brown and red seaweeds[J].Journal of Applied Phycology,2007,19(2):153-160.
[21]Lahrsen E,Liewert I,Alban S.Gradual degradation of fucoidan from Fucus vesiculosus,and its effect on structure,antioxidant and antiproliferative activities[J].Carbohydrate Polymers,2018,192:208-216.
[22]Vo T S,Kim S K.Fucoidans as a natural bioactive ingredient for functional foods[J].Journal of Functional Foods,2013,5(1):16-27.
[23]张加幸,苗钧魁,冷凯良.收获期内不同品种鲜海带中岩藻多糖含量、组成及其抗氧化活性研究[J].应用化工,2016,45(11):2186-2189.
[24]Marudhupandi T.Antibacterial effect of fucoidan from Sargassum wightii against the chosen human bacterial pathogens[J].International Current Pharmaceutical Journal,2013,2(10):156-158.
[25]Liu M,Liu Y,Cao M J,et al.Antibacterial activity and mechanisms of depolymerized fucoidans isolated from Laminaria japonica[J].Carbohydrate Polymers,2017,172:294-305.
[26]Feng H,Ying Y,Yang G A,et al.Studies on antibacterial activity and antibacterial mechanism of a novel polysaccharide from Streptomyces virginia H03[J].Food Control,2010,21(9):1257-1262.
[27]Yamashita S,Sugita-Konishi Y,Shimizu M.In vitro bacteriostatic effects on dietary polysaccharides[J].Food Science & Technology International Tokyo,2001,7(3):262-264.
[28]Hwang P A,Hung Y L,Phan N N,et al.The in vitro and in vivo effects of the low molecular weight fucoidan on the bone osteogenic differentiation properties[J].Cytotechnology,2016,68(4):1349-1359.
[29]石磊,高欣,赵艳威,等.岩藻多糖对小鼠免疫功能的影响[J].食品研究与开发,2012,33(12):194-196.
[30]Yuguchi Y,Tran V T,Bui L M,et al.Primary structure,conformation in aqueous solution,and intestinal immunomodulating activity of fucoidan from two brown seaweed species Sargassum crassifolium and Padina australis[J].Carbohydrate Polymers,2016,147:69-78.
[31]Khilaa Chenko S,Zaporozhets T,Shevchenko N,et al.Immunostimulatory activity of fucoidan from the brown alga Fucus evanescens:Role of sulfates and acetates[J].Journal of Carbohydrate Chemistry,2011,30(4-6):291-305.
[32]Vishchuk O S,Ermakova S P,Zvyagintseva T N.The fucoidans from brown algae of Far-Eastern seas:Anti-tumor activity and structure-function relationship[J].Food Chemistry,2013,141(2):1211-1217.
[33]Usoltseva R V,Anastyuk S D,Ishina I A,et al.Structural characteristics and anticancer activity in vitro,of fucoidan from brown algae Padina boryana[J].Carbohydrate Polymers,2018,184:260-268.
[34]Silchenko A S,Rasin A B,Kusaykin M I,et al.Modification of native fucoidan from Fucus evanescens by recombinant fucoidanase from marine bacteria Formosa algae[J].Carbohydrate Polymers,2018,193:189-195.
[35]Vo T S,Kim S K.Fucoidans as a natural bioactive ingredient for functional foods[J].Journal of Functional Foods,2013,5(1):16-27.
[36]Chandía N P,Matsuhiro B.Characterization of a fucoidan from Lessonia vadosa(Phaeophyta)and its anticoagulant and elicitor properties[J].International Journal of Biological Macromolecules,2008,42(3):235-240.
[37]Nishino T,Aizu Y,Nagumo T.The influence of sulfate content and molecular weight of a fucan sulfate from the brown seaweed Ecklonia kurome on its antithrombin activity[J].Thrombosis Research,1991,64(6):723-731.
[38]Park H Y,Han M H,Park C,et al.Anti-inflammatory effects of fucoidan through inhibition of NF-B,MAPK and Akt activation in lipopolysaccharide-induced BV2 microglia cells[J].Food and Chemical Toxicology,2011,49,1745-1752.
[39]Park S B,Chun K R,Kim J K,et al.The differential effect of high and low molecular weight fucoidans on the severity of collagen-induced arthritis in mice[J].Phytotherapy Research,2010,24(9):1384-1391.
[40]Jiao G,Yu G,Zhang J,et al.Chemical structures and bioactivities of sulfated polysaccharides from Marine algae[J].Marine Drugs,2011,9(2):196-223.
[41]Sinha S,Astani A,Ghosh T,et al.Polysaccharides from Sargassum tenerrimum:Structural features,chemical modification and anti-viral activity[J].Phytochemistry,2010,71(2-3):235-242.
[42]Mandal P,Mateu C G,Chattopadhyay K,et al.Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica[J].Antiviral Chemistry & Chemotherapy,2007,18(3):153-162.