摘要
建立了PEG 4000/NaH_2PO_4和PEG4000/(NH_4)_2SO_4两种双水相体系,比较了两种双水相体系对牛血清白蛋白萃取行为的影响.考察了无机盐种类和浓度、PEG质量分数、牛血清白蛋白浓度等因素对双水相形成以及对牛血清白蛋白分配行为的影响.结果表明PEG 4000/NaH_2PO_4双水相系统的最佳萃取条件为:PEG4000=11.50%,NaH_2PO_4=20%,此时K值最小为0.45,达最大回收率.PEG4000/(NH_4)_2SO_4双水相系统最佳萃取条件为:PEG4000=12%,(NH4)_2SO_4=17.50%,此时K值最小为0.42,达最大回收率.
The PEG4000/NaH_2PO_4 and PEG4000/(NH_4)_2SO_4 aqueous two-phase system is established and the influence of different aqueous two-phase system on bovine serum albumin(BSA)extraction behavior is compared.The sorts and concentration of inorganic salt,PEG mass fraction,and the influence of the concentration of BSA on aqueous two-phase system formation as well as on the distribution behavior of BSA are also investigated.The results show that PEG PEG4000/NaH_2PO_4 optimum extraction condition of aqueous two-phase systems is:PEG4000=10.50%,NaH_2PO_4=20%.When the minimum value of Kis 0.45,the recovery rate reaches the highest.PEG4000/(NH_4)_2SO_4 aqueous two-phase system optimum separation condition is as follows:PEG4000=12%,(NH_4)_2SO_4=17.50%.When the minimum value of Kis 0.42,the recovery rate reaches the highest.
引文
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