UPLC-MS/MS MRM模式同时测定参白方中人参皂苷Re、人参皂苷Rg1、人参皂苷Rb1以及Siderin 4种成分含量
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摘要
目的:建立参白方中人参皂苷Re、人参皂苷Rg1、Siderin以及人参皂苷Rb1 UPLC-MS/MS含量测定方法。方法:采用Waters ACQUITY UPLCTM HSS T3(2.1 mm×100 mm,1.8μm)色谱柱;流动相:A为0.1%甲酸甲醇,B为0.1%甲酸水,梯度洗脱。采用电喷雾电离源(ESI),多反应离子监测(MRM)扫描模式。结果:参白方中被检测的4种成分线性关系良好;精密度,重复性,稳定性良好;加样回收率在99.86%~100.97%,RSD 0.91%~2.74%。结论:该方法简单、准确、灵敏、重复性好,可用于参白方主要成分的含量测定。
Objective: To establish an UPLC-MS/MS method for the determination of ginsenoside Re,ginsenoside Rg1,siderin and ginsenoside Rb1 in Shenbai formula. Methods: Waters ACQUITY UPLCTM HSS T3 column(2. 1 mm × 100 mm,1. 8 μm) was adopted; mobile phase: 0. 1% formic acid/methanol and 0. 1% formic acid/water in a gradient mode; electrospray ionization(ESI) and multi reaction ion monitoring(MRM) scanning were applied. Results: Four compounds showed a good linear relationship with good precision,repeatability and stability; the recovery rate was99. 86% ~ 100. 97%,and RSD was 0. 91% ~ 2. 74%. Conclusion: This method is simple,accurate,sensitive and reproducible. It can be used for the content determination of the main constituents in Shenbai formula.
Objective: To establish an UPLC-MS/MS method for the determination of ginsenoside Re,ginsenoside Rg1,siderin and ginsenoside Rb1 in Shenbai formula. Methods: Waters ACQUITY UPLCTM HSS T3 column(2. 1 mm × 100 mm,1. 8 μm) was adopted; mobile phase: 0. 1% formic acid/methanol and 0. 1% formic acid/water in a gradient mode; electrospray ionization(ESI) and multi reaction ion monitoring(MRM) scanning were applied. Results: Four compounds showed a good linear relationship with good precision,repeatability and stability; the recovery rate was99. 86% ~ 100. 97%,and RSD was 0. 91% ~ 2. 74%. Conclusion: This method is simple,accurate,sensitive and reproducible. It can be used for the content determination of the main constituents in Shenbai formula.
引文
[1]曹洪欣.参白汤治疗病毒性心肌炎后遗症的临床观察[J].中医药学报,1993,21(4):27-28.
[2]古平,何民.黄芪总苷治疗小鼠柯萨奇B3病毒性心肌炎的实验研究[J].西南国防医药,2007,17(3):281-284.
[3]程志清,徐田红,高瑞兰,等.人参皂甙对急性病毒性心肌炎小鼠穿孔素表达的干预作用研究[J].中医药学刊,2003,21(3):350-353.
[4]Miaomiao Jiang,Liyuan Kang,Yi Wang,et al.A metabonomic study of cardioprotection of ginsenosides,schizandrin,and ophiopogonin D against acute myocardial infarction in rats[J].BMC Complement Altern Med,2014,14:350.
[5]中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:99.
[6]路金才,孙启时,王明雷,等.白茅根中芦竹素和白茅素的含量测定[J].沈阳药科大学学报,1996,13(4):290-291.
[7]王海侠,姚青春,时维静,等.白茅根中绿原酸的提取与含量测定[J].中国中医药科技,2010,17(4):331-332.
[8]赵燕燕,曹悦,孙启时.RP-HPLC法测定白茅根中siderin含量[J].沈阳药科大学学报,2007,24(2):86-88.
[2]古平,何民.黄芪总苷治疗小鼠柯萨奇B3病毒性心肌炎的实验研究[J].西南国防医药,2007,17(3):281-284.
[3]程志清,徐田红,高瑞兰,等.人参皂甙对急性病毒性心肌炎小鼠穿孔素表达的干预作用研究[J].中医药学刊,2003,21(3):350-353.
[4]Miaomiao Jiang,Liyuan Kang,Yi Wang,et al.A metabonomic study of cardioprotection of ginsenosides,schizandrin,and ophiopogonin D against acute myocardial infarction in rats[J].BMC Complement Altern Med,2014,14:350.
[5]中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:99.
[6]路金才,孙启时,王明雷,等.白茅根中芦竹素和白茅素的含量测定[J].沈阳药科大学学报,1996,13(4):290-291.
[7]王海侠,姚青春,时维静,等.白茅根中绿原酸的提取与含量测定[J].中国中医药科技,2010,17(4):331-332.
[8]赵燕燕,曹悦,孙启时.RP-HPLC法测定白茅根中siderin含量[J].沈阳药科大学学报,2007,24(2):86-88.