小叶榕药效学初步考察
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摘要
目的根据咳特灵的功效建立动物模型,初步探讨咳特灵中小叶榕的药效。方法通过小鼠氨水引咳实验、小鼠气管酚红实验、豚鼠平喘实验、小鼠耳片肿胀实验、大鼠足趾肿胀实验,分别观察咳特灵中小叶榕止咳、祛痰、平喘、抗炎效果。结果 (1)小鼠止咳实验:各给药组的咳嗽潜伏期和咳嗽次数与阴性对照组比较差异均有统计学意义(P<0.05),给药低、中、高剂量组均对小鼠咳嗽潜伏期有不同程度的延长,咳嗽次数增加(P<0.05)。(2)小鼠祛痰实验:给药低、中、高剂量组与阴性对照组比较差异均有统计学意义(P <0. 05)。(3)豚鼠引喘实验:给药中、高剂量组与阴性对照组比较差异有统计学意义(P<0.05),阳性组与阴性对照组比较均有统计学意义(P<0.05),给药低剂量组与阴性对照组比较差异无统计学意义(P>0.05)。(4)小鼠、大鼠抗炎实验:各给药组与阴性对照组比较差异有统计学意义(P<0.05)。结论咳特灵中所含的小叶榕干浸膏具有止咳、祛痰、抗炎、平喘等药效,咳特灵能治疗各种咳嗽症状,可能是小叶榕具有多种药效共同作用的结果。
Objective To explore the pharmacodynamic effects of Ficus microcarpa on animal models in the light of the efficacy of Keteling. Methods The mouse ammonia cough test,mouse tracheal phenol red test,guinea pig asthma test,mouse ear swelling test and rat toe swelling test were established. The effects of relieving cough,dispelling phlegm,anti-asthmatic and anti-inflammation of Ficus microcarpa were observed.Results (1) Mice antitussive test: The cough latency and cough frequency of each drug-administered group was significantly different from that of the model group( P < 0. 05). The low,medium and high doses of experimental groups had different degrees of prolongation of cough latency and increased cough frequency in mice( P<0.05).( 2) Mice expectorant test: The difference was statistically significant between the drugadministered groups and the model group( P<0.05).( 3) Guinea pigs asthma experiment: The middle and high doses of the drug-administered groups were significantly different from the model group( P < 0. 05).The difference between the western medicine group and the model group was statistically significant( P <0.05). There was no significant difference between the low dose group and the model group( P > 0. 05).( 4) Anti-inflammatory experiments in mice and rats: There was a statistically significant difference between the drug-administered groups and the model group( P < 0. 05).. Conclusion The dried extract of Ficus microcarpa in Keteling exhibits relieving cough,dispelling phlegm,anti-inflammatory and anti-asthmatic effects. Keteling has been applied into many kinds of cough symptoms,which may be related to the multiple efficacy of Ficus microcarpa.
Objective To explore the pharmacodynamic effects of Ficus microcarpa on animal models in the light of the efficacy of Keteling. Methods The mouse ammonia cough test,mouse tracheal phenol red test,guinea pig asthma test,mouse ear swelling test and rat toe swelling test were established. The effects of relieving cough,dispelling phlegm,anti-asthmatic and anti-inflammation of Ficus microcarpa were observed.Results (1) Mice antitussive test: The cough latency and cough frequency of each drug-administered group was significantly different from that of the model group( P < 0. 05). The low,medium and high doses of experimental groups had different degrees of prolongation of cough latency and increased cough frequency in mice( P<0.05).( 2) Mice expectorant test: The difference was statistically significant between the drugadministered groups and the model group( P<0.05).( 3) Guinea pigs asthma experiment: The middle and high doses of the drug-administered groups were significantly different from the model group( P < 0. 05).The difference between the western medicine group and the model group was statistically significant( P <0.05). There was no significant difference between the low dose group and the model group( P > 0. 05).( 4) Anti-inflammatory experiments in mice and rats: There was a statistically significant difference between the drug-administered groups and the model group( P < 0. 05).. Conclusion The dried extract of Ficus microcarpa in Keteling exhibits relieving cough,dispelling phlegm,anti-inflammatory and anti-asthmatic effects. Keteling has been applied into many kinds of cough symptoms,which may be related to the multiple efficacy of Ficus microcarpa.
引文
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[8]黄华花,陈景海.小叶榕止咳化痰有效部位筛选及其化学成分的UHPLC-MS分析[J].中医药导报,2017,15(1):54-58.
[9]陆月明.咳嗽的动物模型研究方法[J].临床肺科杂志,2007,12(5):480-481.
[10]LV Hongming,YU Zhenxiang,ZHENG Yuwei,et al.Isovitexin exerts anti-inflammatory and anti-oxidant activities on lipopolysaccharide-induced acute lung injury by inhibiting MAPK and NF-κB and activating HO-1/Nrf2 pathways[J].Int J Biol Sci,2016,12(1):72-86.
[2]吕凯结.咳特灵治疗感冒后咳嗽疗效观察[J].临床医药文献电子杂志,2017,4(41):8051-8051.
[3]王玮,朱少璇,卢丹,等.中西药复方咳特灵的研究进展[C]//传统医药国际科技大会论文集.广州:2009:76-80.
[4]陈路,蓝鸣生,王硕.小叶榕不同提取物的主要药效学研究[J].广西植物,2009,29(6):871-874.
[5]陈艳芬,江涛,唐春萍,等.小叶榕不同提取物镇咳祛痰作用的比较研究[J].中医药导报,2010,16(7):98-99.
[6]方道硕.小叶榕叶总黄酮的抗炎镇痛作用研究[J].中国药房,2012,39(8):3673-3675.
[7]刘力恒,王立升,王天文,等.小叶榕化学成分及药理活性的研究进展[J].时珍国医国药,2008,19(2):390-392.
[8]黄华花,陈景海.小叶榕止咳化痰有效部位筛选及其化学成分的UHPLC-MS分析[J].中医药导报,2017,15(1):54-58.
[9]陆月明.咳嗽的动物模型研究方法[J].临床肺科杂志,2007,12(5):480-481.
[10]LV Hongming,YU Zhenxiang,ZHENG Yuwei,et al.Isovitexin exerts anti-inflammatory and anti-oxidant activities on lipopolysaccharide-induced acute lung injury by inhibiting MAPK and NF-κB and activating HO-1/Nrf2 pathways[J].Int J Biol Sci,2016,12(1):72-86.