CRISPR/Cas9系统及其在单子叶植物中的应用
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摘要
CRISPR/Cas9系统作为第3代人工核酸内切酶,已经成为继锌指核酸内切酶(zinc finger endonuclease,简称ZFNs)和类转录激活因子效应物核酸酶(transcription activator-like effector nuclease,简称TALENs)之后的新型高效定点的基因组编辑新技术。作为新型的基因编辑技术,CRISPR/Cas9系统拥有突变效率高、构建简单、花费成本低等特点,自其出现之后,受到广泛关注且得到迅速发展,给植物基因组研究和遗传育种带来革命性的变革。目前,该技术已经在多种单子叶植物中实现了基因组定点精确编辑,包括水稻(Oryza sativa)、小麦(Triticum aestivum)、玉米(Zea mays)等单子叶植物。
引文
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[31]Jiang W Z,Zhou H B,Bi H H,et al.Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis,tobacco,sorghum and rice[J].Nucleic Acids Research,2013,41(20):e188.
[32]Wang Y P,Cheng X,Shan Q W,et al.Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew[J].Nature Biotechnology,2014,32(9):947-951.
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[34]Liang Z,Zhang K,Chen K L,et al.Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas9 system[J].Journal of Genetics and Genomics,2014,41(2):63-68.
[35]Xing H L,Dong L,Wang Z P,et al.A CRISPR/Cas9 toolkit for multiplex genome editing in plants[J].BMC Plant Biology,2014,14(1):327.
[36]Zetsche B,Gootenberg J S,Abudayyeh O O,et al.Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system[J].Cell,2015,163(3):759-771.
[2]Durai S,Mani M,Kandavelou K,et al.Zinc finger:nucleases customdesigned molecular scissors for genome engineering of plant and mammalian cells[J].Nucleic Acids Research,2005,33(18):5978-5990.
[3]Miller J C,Tan S Y,Qiao G J,et al.A TALE nuclease architecture for efficient genome editing[J].Nature Biotechnology,2011,29(2):143-148.
[4]Lillestol R,Redder P,Garrett R A,et al.A putative viral defence mechansm in archaeal cells[J].Archaea,2006,2(1):59-72.
[5]Brouns S J J,Jore M M,Lundgren M,et al.Small CRISPR RNAs guide antiviral defense in prokaryotes[J].Science,2008,321(5891):960-964.
[6]Ishino Y,Shinagawa H,Makino K,et al.Nucleotide sequence of the iap gene,responsible for alkaline phosphatase isozyme conversion in Escherichia coli,and identification of the gene product[J].Journal of Bacteriology,1987,169(12):5429-5433.
[7]Chrisyian M,Cermak T,Doyle E L,et al.Targeting DNA doublestrand breaks with TAL effector nucleases[J].Genetics,2010,186(2):757-761.
[8]Carroll D,Morton J J,Beumer K J,et al.Design,construction and in vitro testing of zinc finger nuclease[J].Nature Protocols,2006,1(3):1329-1341.
[9]Grissa I,Vergnaud G,Pourcel C.The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats[J].BMC Bioinformat,2007,8(1):172.
[10]Grissa I,Vergnaud G,Pourcel C.CRISPR finder:a web tool to identify clustered regularly interspaced short palindromic repeats[J].Nucleic Acids Research,2007,35:52-57.
[11]Jansen R,van Embden J D A,Gaastra W,et al.Identification of genes that are associated with DNA repeats in prokaryotes[J].Molecular microbiology,2002,43(6):1565-1575.
[12]Wei C X,Liu J Y,Yu Z S,et al.TALEN or Cas9-rapid,efficient and specific choices for genome modifications[J].Journal of Genetics and Genomics,2013,40(6):281-289.
[13]Godde J S,Bickerton A.The repetitive DNA elements called CRISPRs and their associated genes:evidence of horizontal transfer among prokaryotes[J].Journal of Molecular Evolution,2006,62(6):718-729.
[14]Haft D H,Selengut J,Mongodin E F,et al.A guild of 45 CRISPRassociated(Cas)protein families and multiple CRISPR/Cas9subtypes exist in prokaryotic genomes[J].PLo S Computational Biology,2005,1(6):e60.
[15]Makarova KS,Aravind L,Wolf Y I,et al.Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR/Cas9 systems[J].Biology Direct,2011,6(1):38.
[16]Garneau J E,Dupuis M,Villion M,et al.The CRISPR/Cas9bacterial immune system cleaves bacteriophage and plasmid DNA[J].Nature,2010,468(7320):67-71.
[17]Cong L,Ran F A,Cox D,et al.Multiple genome engineering using CRISPR/Cas9 systems[J].Science,2013,339(6121):819-923.
[18]Qi L S,Larson M H,Gilbert L A,et al.Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression[J].Cell,2013,152(5):1173-1183.
[19]Wang H Y,Yang H,Shivalila C S,et al.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas9mediated genome engineering[J].Cell,2013,153(4):910-918.
[20]Terns M P,Terns R M.CRISPR-based adaptive immune systems[J].Current Opinion in Microbiology,2011,14(3):321-327.
[21]Jinek M,Chylinski K,Fonfara I,et al.A programmable dual-RNAguided DNA endonuclease in adaptive bacterial immunity[J].Science,2012,337(6096):816-821.
[22]Shan Q W,Gao C X.Research progress of genome editingand derivative technologies in plants[J].Hereditas,2015,37(10):953-973.
[23]Xie K B,Yang Y N.RNA-guided genome editing in plants using a CRISPR-Cas system[J].Molecular Plant,2013,6(6):1975-1983.
[24]Mao Y F,Zhang H,Xu N F,et al.Application of the CRISPR-Cas system for efficient genome engineering in plants[J].Molecular Plant,2013,6(6):2008-2011.
[25]Feng Z Y,Zhang B T,Ding W N,et al.Efficient genome editing in plants using a CRISPR/Cas9 system[J].Cell Research,2013,23(10):1229-1232.
[26]Ma X L,Zhang Q Y,Zhu Q L,et al.A robust CRISPR/Cas9 system for convenient,high-efficiency multiplex genome editing in monocot and dicot plants[J].Molecular Plant,2015,8(8):1274-1284.
[27]Engler C,Kandzia R,Marillonnet S.A one pot,one step,precision cloning method with high throughput capability[J].PLo S One,2008,3(11):e3647.
[28]Gibson D G,Young L,Chuang R Y,et al.Enzymatic assembly of DNA molecules up to several hundred kilobases[J].Nature Methods,2009,6(5):343-345.
[29]Orel N,Kyryk A,Puchta H.Different pathways of homologous recombination are used for the repair of double-strand breaks within tandemly arranged sequences in the plant genome[J].The Plant Journal,2003,35(5):604-612.
[30]Frampton R A,Pitman A R,Fineran P C.Advances in bacteriophagemediated control of plant pathogens[J].International Journal of Microbiology,2012(6079):1-11.
[31]Jiang W Z,Zhou H B,Bi H H,et al.Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis,tobacco,sorghum and rice[J].Nucleic Acids Research,2013,41(20):e188.
[32]Wang Y P,Cheng X,Shan Q W,et al.Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew[J].Nature Biotechnology,2014,32(9):947-951.
[33]Sharma S,Upadhyay S K.Functional characterization of expressed sequence tags of bread wheat(Triticum aestivum)and analysis of crispr binding sites for targeted genome editing[J].American Journal of Bioinformatics Research,2014,4(1):11-22.
[34]Liang Z,Zhang K,Chen K L,et al.Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas9 system[J].Journal of Genetics and Genomics,2014,41(2):63-68.
[35]Xing H L,Dong L,Wang Z P,et al.A CRISPR/Cas9 toolkit for multiplex genome editing in plants[J].BMC Plant Biology,2014,14(1):327.
[36]Zetsche B,Gootenberg J S,Abudayyeh O O,et al.Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system[J].Cell,2015,163(3):759-771.