不同栽培基质对金线莲3种基原植物生长及其活性成分含量的影响
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摘要
以3种不同基原的金线莲为试验材料,研究3种栽培基质(泥炭-河沙-花生壳)配比对金线莲移栽成活率、株高、径粗、植株鲜重、根数、最长根长度、根直径以及多糖、黄酮和多酚含量的影响。结果表明,当基质配比为4∶2∶2时,3种不同基原金线莲移栽存活率最高而且株高、径粗、植株鲜重、最长根长度、根直径最大。基质配比对金线兰和台湾银线兰的多糖含量没有显著影响。基质配比为4∶2∶2时,滇越金线兰中多糖含量显著低于其他2个组合;基质配比为4∶2∶1时,台湾银线兰的黄酮含量高于其他2种基质配比;基质配比为4∶2∶2时,台湾银线兰和滇越金线兰的多酚含量较高。
Three different origins of Anoectochilus roburghii were used as experimental materials to study the effect of three different substrate( peat soil-river sand-peanut shell) radio on survival rate,plant height,stem diameter,plant fresh weight,root number,the longest root length,root diameter,and the contents of polysaccharide,flavonoids,and polyphenol. The results showed that when the substrate ratio was 4 ∶2 ∶2,the survival rate of A. roburghii from different origins was the highest,and the plant height,stem diameter,plant fresh weight,the longest root length and root diameter were also the largest. The cultivation substrate had no significant effect on the polysaccharide content of A. roxburghii and A. formosanus. When the substrate ratio was 4 ∶ 2 ∶ 2,the polysaccharide content of A.chapaensis was significantly lower than that of the other two combinations. When the substrate ratio was 4 ∶2 ∶1,the flavonoid content of A. formosanus was higher than that of the other two combinations. When the substrate ratio was 4 ∶2 ∶2,A. formosanus and A. chapaensis had higher polyphenol content.
Three different origins of Anoectochilus roburghii were used as experimental materials to study the effect of three different substrate( peat soil-river sand-peanut shell) radio on survival rate,plant height,stem diameter,plant fresh weight,root number,the longest root length,root diameter,and the contents of polysaccharide,flavonoids,and polyphenol. The results showed that when the substrate ratio was 4 ∶2 ∶2,the survival rate of A. roburghii from different origins was the highest,and the plant height,stem diameter,plant fresh weight,the longest root length and root diameter were also the largest. The cultivation substrate had no significant effect on the polysaccharide content of A. roxburghii and A. formosanus. When the substrate ratio was 4 ∶ 2 ∶ 2,the polysaccharide content of A.chapaensis was significantly lower than that of the other two combinations. When the substrate ratio was 4 ∶2 ∶1,the flavonoid content of A. formosanus was higher than that of the other two combinations. When the substrate ratio was 4 ∶2 ∶2,A. formosanus and A. chapaensis had higher polyphenol content.
引文
[1]罗晓青,吴明开,查兰松,等.珍稀药用植物金线莲研究现状与发展趋势[J].贵州农业科学,2011,39(3):71.
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[16]王伟,苏明华,李惠华,等.蓝光补光对福建金线莲生长及药用成分的影响[J].热带作物学报,2018,39(5):894.
[17]黄瑞平,黄颖桢,陈菁瑛,等.不同月龄金线莲多糖和总黄酮含量的比较[J].热带生物学报,2012,3(2):174.
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[20]程安玮,吴剑夫,秦宏伟,等. 4种豆类中多酚、类黄酮含量及抗氧化活性研究[J].中国粮油学报,2017,32(10):28.
[21]孔祥海.“药王”金线莲的自然资源初步研究[J].中草药,2001,32(2):155.
[2] Liu Q,Ha W,Liu Z,et al. 3-Hydroxybutanolide derivatives and flavonoid glucosides from Anoectochilus roxburghii[J]. Phytochem Lett,2014,8(1):109.
[3] Shao Q S,Deng Y M,Liu H B,et al. Essential oils extraction from Anoectochilus roxburghii using supercritical carbon dioxide and their antioxidant activity[J]. Ind Crop Prod,2014,60:104.
[4]何春年,王春兰,郭顺星,等.兰科开唇兰属植物的化学成分和药理活性研究进展[J].中国药学杂志,2004,40(2):81.
[5] Cui S C,Yu J,Zhang X H,et al. Antihyperglycemic and antioxidant activity of water extract from Anoectochilus roxburghii in experimental diabetes[J]. Exp Toxicol Pathol,2013,65(5):485.
[6] Du X M,Nobuto I,Norihiro F,et al. Pharmacologically active compounds in the Anoectochilus and Goodyera species[J]. J Nat Med-Tokyo,2008,62(2):132.
[7]邵清松,叶申怡,周爱存,等.金线莲种苗繁育及栽培模式研究现状与展望[J].中国中药杂志,2016,41(2):160.
[8]杨成行,李晓婷,袁建振,等.金线莲组织培养技术研究进展[J].草业科学,2018,35(5):1047.
[9]靳松,陈泽斌,夏体渊,等.金线莲试管苗高效快繁技术研究[J].时珍国医国药,2016,27(6):1491.
[10]魏翠华,谢宇,秦建彬,等.台湾金线莲组培苗定植规格及栽培基质筛选[J].南方农业学报,2016,47(1):92.
[11]余松金,施宗强.金线莲栽培基质配比试验[J].现代农业科技,2016(13):86.
[12]甘金佳,蒋水元,李虹,等.不同栽培基质对金线莲生长和主要活性成分的影响[J].江苏农业科学,2017,45(14):112.
[13]邵清松,周爱存,黄瑜秋,等.不同移栽条件对金线莲组培苗成活率及生长的影响[J].中国中药杂志,2014,39(6):955.
[14]陈湘瑜,徐日荣,唐兆秀.发酵花生壳在金线莲栽培中的应用[J].安徽农业科学,2016,44(32):129.
[15]肖小华,林彩霞,吴序栎,等.金线莲的化学成分及生物活性研究进展[J].现代食品科技,2018,34(5):267.
[16]王伟,苏明华,李惠华,等.蓝光补光对福建金线莲生长及药用成分的影响[J].热带作物学报,2018,39(5):894.
[17]黄瑞平,黄颖桢,陈菁瑛,等.不同月龄金线莲多糖和总黄酮含量的比较[J].热带生物学报,2012,3(2):174.
[18]段红波,宦臣臣,梁引库,等.蒲公英叶脂溶性成分的GC-MS分析及其活性探讨[J].中国实验方剂学杂志,2018,24(1):60.
[19]陈克克,强毅.费菜不同部位脂溶性成分的GC-MS比较[J].中国实验方剂学杂志,2017,23(20):77.
[20]程安玮,吴剑夫,秦宏伟,等. 4种豆类中多酚、类黄酮含量及抗氧化活性研究[J].中国粮油学报,2017,32(10):28.
[21]孔祥海.“药王”金线莲的自然资源初步研究[J].中草药,2001,32(2):155.