芸苔素内酯可提高玉米幼苗的抗旱性
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摘要
本文研究了24-表芸苔素内酯、28-高芸苔素内酯、28-表高芸苔素内酯对干旱胁迫下玉米幼苗抗旱性的调控作用。通过3种芸苔素内酯处理喷雾,可以明显缓解干旱胁迫对玉米幼苗的影响,其中28-高芸苔素内酯在0.03 mg·kg~(-1)处理时调节效果最明显。研究表明,外施芸苔素内酯可以促进干旱胁迫下玉米幼苗生物量的增长,增强玉米幼苗的根系活力,提高光合色素含量和光合性能,提高抗氧化酶活性和脯氨酸含量,降低MDA含量,从而提高玉米幼苗的抗旱性。
In this paper, we studied the regulation of 24-epibrassinolide, 28-homobrassinolide and 28-epihombrassinolide on drought resistance of maize seedlings under drought stress. The effects of drought stress on maize seedlings could be alleviated by three kinds of brassinolide treatment sprays. Among them, 28-homobrassinolide was most effective in the treatment of 0.03 mg·kg~(-1). The research showed that exogenous brassinolide could promote the growth of maize seedling biomass under drought stress, enhance the root activity of maize seedlings, increase photosynthetic pigment content and photosynthetic performance, and increase antioxidant enzyme activity. The content of proline reduced the content of MDA, thereby increasing the drought resistance of maize seedlings.
In this paper, we studied the regulation of 24-epibrassinolide, 28-homobrassinolide and 28-epihombrassinolide on drought resistance of maize seedlings under drought stress. The effects of drought stress on maize seedlings could be alleviated by three kinds of brassinolide treatment sprays. Among them, 28-homobrassinolide was most effective in the treatment of 0.03 mg·kg~(-1). The research showed that exogenous brassinolide could promote the growth of maize seedling biomass under drought stress, enhance the root activity of maize seedlings, increase photosynthetic pigment content and photosynthetic performance, and increase antioxidant enzyme activity. The content of proline reduced the content of MDA, thereby increasing the drought resistance of maize seedlings.
引文
Anjum SA,Wang LC,Farooq M,et al(2011).Brassinolide application improves the drought tolerance in maize through modulation of enzymatic antioxidants and leaf gas exchange.J Agron Crop Sci,197:177-185
Ding DY,Zhang LX,Zhu ZW,et al(2018).Effect of leaf spray 2,4-epibrassinolide on drought resistance of tobacco.Chin Tob Sci,39:50-57(in Chinese with English abstract)[丁丹阳,张璐翔,朱智威等(2018).叶面喷施2,4-表油菜素内酯对烟草抗旱性的影响.中国烟草科学,39:50-57]
Fariduddin Q,Yusuf M,Ahmad I,et al(2014).Brassinosteroids and their role in response of plants to abiotic stresses.Biol Plantarum,58:9-17
Gao JF(2006).Experimental Guide of Plant Physiology.Beijing:Higher Education Press,210-217(in Chinese)[高俊凤(2006).植物生理学实验指导.北京:高等教育出版社,210-217]
Guo YY,Liu J,Zhu YL,et al(2018).Responses of photosynthetic and antioxidant enzyme activities in maize leaves to drought stress.Plant Physiol J,54:1839-1846(in Chinese with English abstract)[郭艳阳,刘佳,朱亚利等(2013).玉米叶片光合和抗氧化酶活性对干旱胁迫的响应.植物生理学报,54:1839-1846]
Hu XQ,LI DY,Yan JK,et al(2018).Effects of exogenous methyl jasmonate on water absorption capacity of maize(Zea mays L.)seedling root under drought stress.Plant Physiol J,54:991-998(in Chinese with English abstract)[忽雪琦,李东阳,严加坤等(2018).干旱胁迫下外源茉莉酸甲酯对玉米幼苗根系吸水的影响.植物生理学报,54:991-998]
Li ZG,Gong M(2014).Plant Physiology Comprehensive and Design Experimental Tutorial.Wuhan:Huazhong University of Science and Technology Press(in Chinese)[李忠光,龚明(2014).植物生理学综合性和设计性实验教程.武汉:华中科技大学出版社]
Liu JB,Yang KJ,Shi SB,et al(2012).Maize Cultivation in Northern China.Beijing:China Agricultural Science and Technology Press,21-22(in Chinese with English abstract)[刘京宝,杨克军,石书兵等(2012).中国北方玉米栽培.北京:中国农业科学技术出版社,21-22]
Mcmorris TC,Patil PA,Chavez RG,et al(1994).Synthesis and biological activity of 28-homobrassinolide and analogues.Phytochemistry,36:585-589
Ming H,Hu CS,Zhang YM,et al(2007).Improved extraction methods of chlorophyll from maize.J Maize Sci,15:93-95,99(in Chinese with English abstract)[明华,胡春胜,张玉铭等(2007).浸提法测定玉米叶绿素含量的改进.玉米科学,15:93-95,99]
Ruan YH,Dong SK,Liu LJ,et al(2011).Effects of brassinosteroid on physiological characteristics at flowering stage of soybean under drought stress.Crops,6:33-37(in Chinese with English abstract)[阮英慧,董守坤,刘丽君等(2011).干旱胁迫下油菜素内酯对大豆花期生理特性的影响.作物杂志,6:33-37]
Shan L(2011).To cope rationally with agricultural drought.Agric Res Arid Areas,29:1-5(in Chinese with English abstract)[山仑(2011).科学应对农业干旱.干旱地区农业研究,29:1-5]
Song JX(2017).Effects of plant growth regulators on growth and physiological characteristics of Leymus chinensis under drought stress and transcriptome analysis(dissertation).Chongqing:Southwest University(in Chinese with English abstract)[宋吉轩(2017).干旱胁迫下植物生长调节剂对羊草生长及生理特性的影响与转录组分析(学位论文).重庆:西南大学]
Wang XK,Huang JL(2015).Principles and Techniques of Plant Physiological and Biochemical Experiments.Beijing:Higher Education Press(in Chinese)[王学奎,黄见良(2015).植物生理生化实验原理与技术.北京:高等教育出版社]
Xia XJ,Gao CJ,Song,LX,et al(2014).Role of H2O2 dynamics in brassinosteroid-induced stomatal closure and opening in Solanum lycopersicum.Plant Cell Environ,37:2036-2050
Xiao RX,LüJX,Jia CS,et al(2018).Effect of exogenous brassinosteroid on physiological characteristics of Paeonia ostii‘Fengdan’.Plant Physiol J,54:1417-1425(in Chinese with English abstract)[肖瑞雪,吕静霞,贾长松等(2018).外源油菜素内酯对油用牡丹‘凤丹’生理特性的影响.植物生理学报,54:1417-1425]
Zhang LQ(2013).The affection of brassinolide on the physiological indexes of tomato seedlings.Nor Hortic,(1):1-3(in Chinese with English abstract)[张林青(2013).油菜素内酯对盐胁迫下番茄幼苗生理指标的影响.北方园艺,(1):1-3]
Zhang RH,Zheng YJ,Ma GS,et al(2011).Effects of drought stress on photosynthetic traits and protective enzyme activity inmaize seeding.Acta Ecol Sin,31:1303-1311(in Chinese with English abstract)[张仁和,郑友军,马国胜等(2011).干旱胁迫对玉米苗期叶片光合作用和保护酶的影响.生态学报,31:1303-1311]
Zheng QL,Yang DY,Liu JW,et al(2017).Effects of drought stress on growth and antioxidant system of Pugionium cornutum and P.dolabratum seedlings.Plant Physiol J,53:600-608(in Chinese with English abstract)[郑清岭,杨冬艳,刘建文等(2017).干旱胁迫对沙芥和斧形沙芥幼苗生长及抗氧化系统的影响.植物生理学报,53:600-608]
Zhou XY,Xue HW(2013).Mechanism of cross-talk between auxin and BR.Sci Sin Vitae,43:1047-1053(in Chinese with English abstract)[周晓艺,薛红卫(2013).生长素与油菜素甾醇相互作用机制的研究进展.中国科学:生命科学,43:1047-1053]
Zhu ZB,Lai CY,Zou KX,et al(2015).Research progress of brassinolide and its physiological effects during seed germination.Chin J Trop Agric,35:13-18(in Chinese with English abstract)[朱早兵,赖彩余,邹凯茜等(2015).油菜素内酯及其在种子萌发过程中的生理效应研究进展.热带农业科学,35:13-18]
Ding DY,Zhang LX,Zhu ZW,et al(2018).Effect of leaf spray 2,4-epibrassinolide on drought resistance of tobacco.Chin Tob Sci,39:50-57(in Chinese with English abstract)[丁丹阳,张璐翔,朱智威等(2018).叶面喷施2,4-表油菜素内酯对烟草抗旱性的影响.中国烟草科学,39:50-57]
Fariduddin Q,Yusuf M,Ahmad I,et al(2014).Brassinosteroids and their role in response of plants to abiotic stresses.Biol Plantarum,58:9-17
Gao JF(2006).Experimental Guide of Plant Physiology.Beijing:Higher Education Press,210-217(in Chinese)[高俊凤(2006).植物生理学实验指导.北京:高等教育出版社,210-217]
Guo YY,Liu J,Zhu YL,et al(2018).Responses of photosynthetic and antioxidant enzyme activities in maize leaves to drought stress.Plant Physiol J,54:1839-1846(in Chinese with English abstract)[郭艳阳,刘佳,朱亚利等(2013).玉米叶片光合和抗氧化酶活性对干旱胁迫的响应.植物生理学报,54:1839-1846]
Hu XQ,LI DY,Yan JK,et al(2018).Effects of exogenous methyl jasmonate on water absorption capacity of maize(Zea mays L.)seedling root under drought stress.Plant Physiol J,54:991-998(in Chinese with English abstract)[忽雪琦,李东阳,严加坤等(2018).干旱胁迫下外源茉莉酸甲酯对玉米幼苗根系吸水的影响.植物生理学报,54:991-998]
Li ZG,Gong M(2014).Plant Physiology Comprehensive and Design Experimental Tutorial.Wuhan:Huazhong University of Science and Technology Press(in Chinese)[李忠光,龚明(2014).植物生理学综合性和设计性实验教程.武汉:华中科技大学出版社]
Liu JB,Yang KJ,Shi SB,et al(2012).Maize Cultivation in Northern China.Beijing:China Agricultural Science and Technology Press,21-22(in Chinese with English abstract)[刘京宝,杨克军,石书兵等(2012).中国北方玉米栽培.北京:中国农业科学技术出版社,21-22]
Mcmorris TC,Patil PA,Chavez RG,et al(1994).Synthesis and biological activity of 28-homobrassinolide and analogues.Phytochemistry,36:585-589
Ming H,Hu CS,Zhang YM,et al(2007).Improved extraction methods of chlorophyll from maize.J Maize Sci,15:93-95,99(in Chinese with English abstract)[明华,胡春胜,张玉铭等(2007).浸提法测定玉米叶绿素含量的改进.玉米科学,15:93-95,99]
Ruan YH,Dong SK,Liu LJ,et al(2011).Effects of brassinosteroid on physiological characteristics at flowering stage of soybean under drought stress.Crops,6:33-37(in Chinese with English abstract)[阮英慧,董守坤,刘丽君等(2011).干旱胁迫下油菜素内酯对大豆花期生理特性的影响.作物杂志,6:33-37]
Shan L(2011).To cope rationally with agricultural drought.Agric Res Arid Areas,29:1-5(in Chinese with English abstract)[山仑(2011).科学应对农业干旱.干旱地区农业研究,29:1-5]
Song JX(2017).Effects of plant growth regulators on growth and physiological characteristics of Leymus chinensis under drought stress and transcriptome analysis(dissertation).Chongqing:Southwest University(in Chinese with English abstract)[宋吉轩(2017).干旱胁迫下植物生长调节剂对羊草生长及生理特性的影响与转录组分析(学位论文).重庆:西南大学]
Wang XK,Huang JL(2015).Principles and Techniques of Plant Physiological and Biochemical Experiments.Beijing:Higher Education Press(in Chinese)[王学奎,黄见良(2015).植物生理生化实验原理与技术.北京:高等教育出版社]
Xia XJ,Gao CJ,Song,LX,et al(2014).Role of H2O2 dynamics in brassinosteroid-induced stomatal closure and opening in Solanum lycopersicum.Plant Cell Environ,37:2036-2050
Xiao RX,LüJX,Jia CS,et al(2018).Effect of exogenous brassinosteroid on physiological characteristics of Paeonia ostii‘Fengdan’.Plant Physiol J,54:1417-1425(in Chinese with English abstract)[肖瑞雪,吕静霞,贾长松等(2018).外源油菜素内酯对油用牡丹‘凤丹’生理特性的影响.植物生理学报,54:1417-1425]
Zhang LQ(2013).The affection of brassinolide on the physiological indexes of tomato seedlings.Nor Hortic,(1):1-3(in Chinese with English abstract)[张林青(2013).油菜素内酯对盐胁迫下番茄幼苗生理指标的影响.北方园艺,(1):1-3]
Zhang RH,Zheng YJ,Ma GS,et al(2011).Effects of drought stress on photosynthetic traits and protective enzyme activity inmaize seeding.Acta Ecol Sin,31:1303-1311(in Chinese with English abstract)[张仁和,郑友军,马国胜等(2011).干旱胁迫对玉米苗期叶片光合作用和保护酶的影响.生态学报,31:1303-1311]
Zheng QL,Yang DY,Liu JW,et al(2017).Effects of drought stress on growth and antioxidant system of Pugionium cornutum and P.dolabratum seedlings.Plant Physiol J,53:600-608(in Chinese with English abstract)[郑清岭,杨冬艳,刘建文等(2017).干旱胁迫对沙芥和斧形沙芥幼苗生长及抗氧化系统的影响.植物生理学报,53:600-608]
Zhou XY,Xue HW(2013).Mechanism of cross-talk between auxin and BR.Sci Sin Vitae,43:1047-1053(in Chinese with English abstract)[周晓艺,薛红卫(2013).生长素与油菜素甾醇相互作用机制的研究进展.中国科学:生命科学,43:1047-1053]
Zhu ZB,Lai CY,Zou KX,et al(2015).Research progress of brassinolide and its physiological effects during seed germination.Chin J Trop Agric,35:13-18(in Chinese with English abstract)[朱早兵,赖彩余,邹凯茜等(2015).油菜素内酯及其在种子萌发过程中的生理效应研究进展.热带农业科学,35:13-18]