麻杏石甘合剂对COPD模型大鼠肺部形态功能及炎症因子水平的影响
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摘要
目的研究麻杏石甘合剂对LPS联合香烟熏蒸所诱导的COPD模型大鼠肺部功能的干预作用及作用机制。方法将SD大鼠于实验第1、14天通过气道滴注生理盐水,并于30 d后检测以此作为空白组;另取SD大鼠于实验第1、14天通过气道滴注脂多糖(LPS)200μg/只,并联合香烟烟雾诱导每天2 h,共30 d,建立模型组。其他各实验组在造模的同时分别给予相应受试药物,通过观察各组中大鼠肺部呼吸功能、血清中TNF-α、IL-6含量及肺组织形态学的变化,探讨麻杏石甘合剂对COPD模型大鼠肺功能的干预作用。结果通过与空白组比较,模型组大鼠呼吸功能参数值FVC、FEV0.3、PEF明显下降(P<0.01);大鼠血清中IL-6、TNF-α含量明显升高(P<0.01);通过与模型组比较,麻杏石甘合剂高中剂量组与阳性对照药氨茶碱给药组使大鼠肺部呼吸功能参数值FVC、FEV0.3、PEF得到明显改善(P<0.01或P<0.05);血清中IL-6、TNF-α含量降低(P<0.01或P<0.05);肺组织病变程度减轻。结论通过给予麻杏石甘合剂可通过改善肺组织病变,提高肺部呼吸功能,降低炎性因子的产生来实现其对慢性阻塞性肺疾病的干预及预防作用。
Objective To study the intervention effect and mechanism of Maxing Shigan mixture on lung function of rats with COPD induced by LPS combined with cigarette fumigation. Methods SD rats were instilled with saline on the1 st and 14 th day of the experiment, and were detected after 30 d, which were set as the blank group. SD rats instilled with lipopolysaccharide(LPS) 200 μg/rat by airway on the 1 st and 14 th day of the experiment, combined with cigarette smoke induction with 2 hours a day for 30 d. And model group was established. The other experimental groups were given the corresponding test drugs while modeling. The lung respiratory function, serum TNF-α, IL-6 content and lung tissue morphology change were observed in each group to explore the intervention role of Maxing Shigan mixture on pulmonary function in rats with COPD model. Results Compared with those of the blank group, the respiratory function parameters FVC, FEV0.3, and PEF of the model group were significantly decreased(P<0.01), and the serum levels of IL-6 and TNF-α were significantly increased in the rats of the model group(P<0.01). Compared with the model group,the high-dose group of Maxing Shigan mixture and the positive control drug aminophylline group improved the respirato ry function parameters FVC, FEV0.3 and PEF of the rats(P<0.01 or P<0.05), serum IL-6 and TNF-α levels were de creased(P<0.01 or P<0.05). The extent of lung tissue lesions was reduced. Conclusion By administering Maxing Shigan mixture, it can improve the lung tissue damage, improve lung respiratory function and reduce the production of inflammatory factors to achieve its intervention and prevention of chronic obstructive pulmonary disease.
Objective To study the intervention effect and mechanism of Maxing Shigan mixture on lung function of rats with COPD induced by LPS combined with cigarette fumigation. Methods SD rats were instilled with saline on the1 st and 14 th day of the experiment, and were detected after 30 d, which were set as the blank group. SD rats instilled with lipopolysaccharide(LPS) 200 μg/rat by airway on the 1 st and 14 th day of the experiment, combined with cigarette smoke induction with 2 hours a day for 30 d. And model group was established. The other experimental groups were given the corresponding test drugs while modeling. The lung respiratory function, serum TNF-α, IL-6 content and lung tissue morphology change were observed in each group to explore the intervention role of Maxing Shigan mixture on pulmonary function in rats with COPD model. Results Compared with those of the blank group, the respiratory function parameters FVC, FEV0.3, and PEF of the model group were significantly decreased(P<0.01), and the serum levels of IL-6 and TNF-α were significantly increased in the rats of the model group(P<0.01). Compared with the model group,the high-dose group of Maxing Shigan mixture and the positive control drug aminophylline group improved the respirato ry function parameters FVC, FEV0.3 and PEF of the rats(P<0.01 or P<0.05), serum IL-6 and TNF-α levels were de creased(P<0.01 or P<0.05). The extent of lung tissue lesions was reduced. Conclusion By administering Maxing Shigan mixture, it can improve the lung tissue damage, improve lung respiratory function and reduce the production of inflammatory factors to achieve its intervention and prevention of chronic obstructive pulmonary disease.
引文
[1]曾华东,徐虹,李理,等.慢性阻塞性肺疾病大鼠模型肺泡巨噬细胞炎症及调控机制探讨[J].中国呼吸与危重监护杂志,2011,11(2):133-137.
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[11]孙华启,徐莉娜,王艳娟.玉屏风颗粒联合中药穴位贴敷对慢性阻塞性肺疾病患者肺功能及免疫功能的影响[J].中国中医药科技,2015,22(6):614-615.
[12]常秀娟,孙晓萍,周军,等.芪白平肺颗粒对慢性阻塞性肺疾病动物模型的疗效观察[J].中国实验方剂学杂志,2016,22(2):87-90.
[13]高月娟,刘金丽,王景欣,等.玉屏风颗粒对过敏性哮喘大鼠气道炎症因子的影响[J].中成药,2016,38(11):2466-2467.
[14]罗明,王明航,龚正.麦杏补肺汤治疗慢性阻塞性肺疾病稳定期肺气虚症临床疗效评价[J].中华中医药杂志,2016,31(2):682-684.
[15]刘积锋,钟小宁,何志义,等.CD8+T细胞在大鼠慢性支气管炎中表达及意义[J].医学研究生学报,2015,28(1):16-19.
[16]许扬,张鹏俊,杨汀,等.我国基层慢性阻塞性肺疾病防治现状研究[J].中国全科医学,2016,19(34):4153-4158.
[17]荣蓉,蔡孝桢,周寅川,等.慢性阻塞性肺疾病不同营养状况患者白介素-6检测结果分析[J].内科,2015,10(2):205-206.
[2]陈娜,梁仁.麻杏石甘汤的现代药理研究及其临床应用[J].广东药学院学报,2004,5(20):545-546.
[3]Yang G,Wang Y,Zeng Y,et al.Rapid health transition in China,1990-2010:Findings from the Global Burden of Disease Study 2010[J].Lancet,2013,381(9882):1987-2015.
[4]符子艺,刘小虹,任吉祥,等.痰热清注射液治疗慢性阻塞性肺疾病合并呼吸衰竭的Meta分析[J].中草药,2014,45(6):889-894.
[5]文丹丹,王敏.麻杏石甘汤治疗咳嗽变异性哮喘的研究进展[J].中国实验方剂学杂志,2012,18(8):285-287.
[6]李玲,卢芳国,熊兴耀,等.麻杏石甘汤对A型流感病毒感染小鼠的免疫保护作用[J].中医药学报,2010,38(2):25-28.
[7]Takeuchi Y,Takahashi M,Fuchikami J.Vulnerability of gastric mucosa to prednisol one in rats chronically exposed tocigarettesmoke[J].J Pharmacol Sci,J Pharmacol Sci,2008,106(4):585-592.
[8]吕倩,王昌明,蒋明,等.HIF-1α和VEGF在大鼠COPD中的表达及与肺血管重构的关系研究[J].中国药理学通报,2012,28(6):772-777.
[9]赵甫成.益气健脾补肾活血方对COPD稳定期患者FEV1的影响[J].中国当代医药,2010,17(13):101-102.
[10]张伟,张心月,邵雨萌.玉屏风散对慢性阻塞性肺疾病大鼠核因子κB和γ-谷氨酰半胱氨酸合酶表达的干预作用[J].中医药临床杂志,2006,18(4):369-370.
[11]孙华启,徐莉娜,王艳娟.玉屏风颗粒联合中药穴位贴敷对慢性阻塞性肺疾病患者肺功能及免疫功能的影响[J].中国中医药科技,2015,22(6):614-615.
[12]常秀娟,孙晓萍,周军,等.芪白平肺颗粒对慢性阻塞性肺疾病动物模型的疗效观察[J].中国实验方剂学杂志,2016,22(2):87-90.
[13]高月娟,刘金丽,王景欣,等.玉屏风颗粒对过敏性哮喘大鼠气道炎症因子的影响[J].中成药,2016,38(11):2466-2467.
[14]罗明,王明航,龚正.麦杏补肺汤治疗慢性阻塞性肺疾病稳定期肺气虚症临床疗效评价[J].中华中医药杂志,2016,31(2):682-684.
[15]刘积锋,钟小宁,何志义,等.CD8+T细胞在大鼠慢性支气管炎中表达及意义[J].医学研究生学报,2015,28(1):16-19.
[16]许扬,张鹏俊,杨汀,等.我国基层慢性阻塞性肺疾病防治现状研究[J].中国全科医学,2016,19(34):4153-4158.
[17]荣蓉,蔡孝桢,周寅川,等.慢性阻塞性肺疾病不同营养状况患者白介素-6检测结果分析[J].内科,2015,10(2):205-206.