包头南海湖不同湖区春季沉积物细菌群落结构
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摘要
【目的】探究包头南海湖不同功能区域春季沉积物细菌群落多样性。【方法】采用高通量测序技术对进水口区、旅游开发区、湖心区和水草区共4个采样点表层沉积物细菌进行测定。【结果】不同区域物种丰富度和均匀度表现为水草区>进水口区>湖心区>旅游开发区。从门水平来看,沉积物细菌主要为变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)、酸杆菌门(Acidobacteria)和蓝藻门(Cyanobacteria)等;从属水平分析,优势类群集中在硫杆菌属(Thiobacillus)、norank-f-anaerolineaceae。对沉积物细菌群落与环境因子做相关性分析,TN、TP、TOC对沉积物细菌群落结构影响较大。【结论】Thiobacillus在南海湖春季沉积物中广泛存在,其在地球生化循环中发挥着重要作用,而且极有可能诱发黑臭水体出现,应在以后研究中得到更多关注,并控制TN、TP以及TOC量,为预防南海湖黑臭水体的发生提供理论依据和基础数据。
【Objective】Microbes and their community structure play an key role in aquatic ecosystems and this paper analyzed the spatial variation of the sediment microbial community in Baotou Nanhai Lake in spring.【Method】Sediment samples were taken from deposit surface at four sites: the lake inlet, recreational area, lake center and plant-growing area. The microbial community in each sample was analyzed using the high-throughput sequencing approach.【Result】The plant-growing area had the highest species richness and evenness, followed by the lake inlet area and the lake center. At phyla level, Proteobacteria, Chloroflexi, Bacteroidetes, Acidobacteria and Cyanobacteria were dominant; and Thiobacillus, unclassified-p-Chloroflexi were the major genus. Correlation analysis revealed that TN,TP and TOC had a great impact on structure of the microbial community.【Conclusion】Thiobacillus was widely found and played an important role in biogeochemical processes in spring within the sediments at the Nanhai Lake; It could lead to black and odorous water. Management of TN, TP and TOC should be improved as they mediate the biochemical reactions within the sediment.
【Objective】Microbes and their community structure play an key role in aquatic ecosystems and this paper analyzed the spatial variation of the sediment microbial community in Baotou Nanhai Lake in spring.【Method】Sediment samples were taken from deposit surface at four sites: the lake inlet, recreational area, lake center and plant-growing area. The microbial community in each sample was analyzed using the high-throughput sequencing approach.【Result】The plant-growing area had the highest species richness and evenness, followed by the lake inlet area and the lake center. At phyla level, Proteobacteria, Chloroflexi, Bacteroidetes, Acidobacteria and Cyanobacteria were dominant; and Thiobacillus, unclassified-p-Chloroflexi were the major genus. Correlation analysis revealed that TN,TP and TOC had a great impact on structure of the microbial community.【Conclusion】Thiobacillus was widely found and played an important role in biogeochemical processes in spring within the sediments at the Nanhai Lake; It could lead to black and odorous water. Management of TN, TP and TOC should be improved as they mediate the biochemical reactions within the sediment.
引文
[1]张伟杰,张正亚,徐建新.三峡库区沉积物中重金属化学形态分布特征与相关性分析[J].灌溉排水学报,2017,36(7):86-93.
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[7]薛银刚,江晓栋,孙萌,等.基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究[J].生态与农村环境学报,2017,33(11):992-1 000.
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[12]姜庆宏,王佳宁,李卫平,等.南海湖春季浮游植物群落结构及其与水质因子的关系[J].干旱区资源与环境,2018,32(8):166-171.
[13]谭雪莲,郭天文,张国宏,等.轮套作对马铃薯根际土壤微生物和酶活性的影响[J].灌溉排水学报,2016,35(9):45-50.
[14]谷鹏,焦燕,杨文柱,等.不同灌溉方式对农田土壤微生物丰度及通透性的影响[J].灌溉排水学报,2018,37(1):21-27.
[15]谭雪莲,郭天文,刘高远.马铃薯连作土壤微生物特性与土传病原菌的相互关系[J].灌溉排水学报,2016,35(8):30-35.
[16]薛银刚,刘菲,江晓栋,等.太湖不同湖区冬季沉积物细菌群落多样性[J].中国环境科学,2018,38(2):719-728.
[17]王鹏,肖汉玉,张华,等.鄱阳湖入湖河口沉积物细菌群落特征[J].中国环境科学,2018,38(4):1 481-1 489.
[18]金笑.鄱阳湖表层沉积物细菌群落结构与功能的时空差异研究[D].昆明:云南大学,2014.
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[23]WINTER A D,MARSH T L,BRENDEN T O,et al.Molecular characterization of bacterial communities associated with sediments in the Laurentian Great Lake[J].Journal of Great Lakes Research,2014,40(3):640-645.
[24]LIU F,LIN G,GAO G,et al.Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake,Lake Taihu,as revealed by denaturing gradient gel electrophoresis[J].Journal of Applied Microbiology,2009,106(3):1 022-1 032.
[25]李真,黄民生,何岩,等.铁和硫的形态转化与水体黑臭的关系[J].环境科学与技术,2010,33(6):1-7.
[26]李靖宇,杜瑞芳,赵吉.乌梁素海富营养化湖泊湖滨湿地过渡带细菌群落结构的高通量分析[J].微生物学报,2014,55(5):598-606.
[2]叶雅杰,罗金明,王永洁,等.扎龙湿地底泥沉积物重金属污染评价[J].灌溉排水学报,2012,31(4):139-141.
[3]彭磊,赵建伟,张钰,等.城市富营养化湖泊沉积物微生物多样性季节变化[J].应用与环境生物学报,2015,21(6):1 012-1 018.
[4]SUN Yajun,WANG Tieyu,PENG Xiawei,et al.Bacterial community compositions in sediment polluted by perfluoroalkyl acids(PFAAs)using Illumina high-throughput sequencing[J].Environmental Science and Pollution Research,2016,23(11):10 556-10 565.
[5]SHAO Keqiang,GAO Guang,QIN Boqiang,et al.Structural changes of the sediment bacterial community in Meiliang Bay of Lake Taihu,China,during typhoon Morakot,2009[J].Journal of Basic Microbiology,2012,52(1):99-103.
[6]金笑,寇文伯,于昊天,等.鄱阳湖不同区域沉积物细菌群落结构、功能变化及其与环境因子的关系[J].环境科学研究,2017,30(4):529-536.
[7]薛银刚,江晓栋,孙萌,等.基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究[J].生态与农村环境学报,2017,33(11):992-1 000.
[8]杨文焕,崔亚楠,李卫平,等.包头市南海湖冰封期营养盐和叶绿素a时空分布特征研究[J].灌溉排水学报,2018,37(3):72-77.
[9]杨文焕,齐璐,李卫平,等.包头南海湖冰封期不同形态氮的空间分布[J].东北农业大学学报,2018,49(3):42-40.
[10]杨文焕,崔亚楠,李卫平,等.冰封期湿地沉积物碳、氮、磷分布及污染评价[J].环境化学,2017,30(4):529-536.
[11]于玲红,王晓云,李卫平,等.包头市南海湿地水质现状分析与评价[J].环境化学,2017,36(2):390-396.
[12]姜庆宏,王佳宁,李卫平,等.南海湖春季浮游植物群落结构及其与水质因子的关系[J].干旱区资源与环境,2018,32(8):166-171.
[13]谭雪莲,郭天文,张国宏,等.轮套作对马铃薯根际土壤微生物和酶活性的影响[J].灌溉排水学报,2016,35(9):45-50.
[14]谷鹏,焦燕,杨文柱,等.不同灌溉方式对农田土壤微生物丰度及通透性的影响[J].灌溉排水学报,2018,37(1):21-27.
[15]谭雪莲,郭天文,刘高远.马铃薯连作土壤微生物特性与土传病原菌的相互关系[J].灌溉排水学报,2016,35(8):30-35.
[16]薛银刚,刘菲,江晓栋,等.太湖不同湖区冬季沉积物细菌群落多样性[J].中国环境科学,2018,38(2):719-728.
[17]王鹏,肖汉玉,张华,等.鄱阳湖入湖河口沉积物细菌群落特征[J].中国环境科学,2018,38(4):1 481-1 489.
[18]金笑.鄱阳湖表层沉积物细菌群落结构与功能的时空差异研究[D].昆明:云南大学,2014.
[19]鲍士旦.土壤农化分析:第3版[M].北京:中国农业出版社,2005.
[20]HUSE S M,WELCH D M,MORRISON H G,et al.Ironing out the wrinkles in the rare biosphere through improved OTU clustering[J].Environmental Microbiology,2010,12(7):1 889-1 898.
[21]JAMI E,ISRAEL A,KOTSER A,et al.Exploring the bovine rumen bacterial community from birth to adulthood[J].The International Society for Micro bial Ecology Journal,2013,7(6):1 069-1 079.
[22]TANG Xiangming,LI Lingling,SHAO Keqiang,et al.Pyrosequencing analysis of free-living and attached bacterial communities in Meiliang Bay,Lake Taihu,a large eutrophic shallow lake in China[J].Canadian Journal of Microbiology,2014,61(1):22-31.
[23]WINTER A D,MARSH T L,BRENDEN T O,et al.Molecular characterization of bacterial communities associated with sediments in the Laurentian Great Lake[J].Journal of Great Lakes Research,2014,40(3):640-645.
[24]LIU F,LIN G,GAO G,et al.Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake,Lake Taihu,as revealed by denaturing gradient gel electrophoresis[J].Journal of Applied Microbiology,2009,106(3):1 022-1 032.
[25]李真,黄民生,何岩,等.铁和硫的形态转化与水体黑臭的关系[J].环境科学与技术,2010,33(6):1-7.
[26]李靖宇,杜瑞芳,赵吉.乌梁素海富营养化湖泊湖滨湿地过渡带细菌群落结构的高通量分析[J].微生物学报,2014,55(5):598-606.