益气活血方对大鼠慢性脊髓压迫后脊髓功能恢复的影响
|
摘要
目的:研究益气活血方对大鼠慢性脊髓压迫后脊髓功能恢复的影响。方法:从60只SD大鼠中,随机选取10只作为假手术组:仅暴露椎板,未造成脊髓压迫,以生理盐水进行灌胃;其余的SD大鼠采用自体第7颈椎棘突椎间移植法制造大鼠脊髓压迫模型,根据改进Rivaling的斜板实验的平均度数、Tarlov评分、BBB评分将其分为益气活血方高、中、低浓度组,颈复康组,NS组。益气活血方高、中、低浓度组及颈复康组按0.01 mL/g灌胃给药,NS组及假手术组灌服等量NS,每日给药1次,连续4周。连续灌胃4周,分别在开始灌胃时,灌胃1、2、3、4周时,采用改进Rivaling的斜板实验、改良Tarlov评分、BBB评分等指标监测大鼠运动功能。结果:不同浓度的益气活血方及颈复康均能显著增加Rivaling的斜板实验的平均度数、Tarlov评分、BBB评分,与NS组比较,差异均有统计学意义(P<0.05)。结论:益气活血方具有促进大鼠慢性脊髓压迫模型中脊髓功能恢复的作用。
Objective: To study the effects of YiQi HuoXue prescription on functional recovery of spinal cord of the rats with chronic spinal compression. Methods: Sixty SD rats were chosen and ten rats were selected as sham operation group randomly; only exposing vertebral plate, no spinal compression, intragastric administration of physiological saline; other SD rat model with spinal compression was established by the seventh cervical spine intervertebral transplantation, according to average degrees of reformed Rivaling inclined plate test, Tarlov score and BBB scores, the rats were divided into high, moderate and low concentrations groups of YiQi HuoXue prescription, JingFu Kang group and NS group. The rats in high, moderate and low concentrations groups of YiQi HuoXue prescription and Jing Fu Kang group accepted intragastric administration of the corresponding medicine in the ratio of 0.01 m L/g,NS group and sham operation group received the lavage of equivalent dose of NS, medication once each day, for four weeks consecutively. Motor function of the rats were monitored by adopting reformed Rivaling inclined plate test, Tarlov score and BBB scores before intragastric administration, at the first, second, third and forth week after lavage respectively. Results: YiQi HuoXue prescription and Jing Fu Kang in different concentrations could increase average degrees of reformed Rivaling inclined plate test, Tarlov score and BBB scores, the difference had statistical meaning compared with NS group(P<0.05). Conclusion: YiQi HuoXue prescription could promote functional recovery of spinal cord of the rats with chronic spinal compression.
Objective: To study the effects of YiQi HuoXue prescription on functional recovery of spinal cord of the rats with chronic spinal compression. Methods: Sixty SD rats were chosen and ten rats were selected as sham operation group randomly; only exposing vertebral plate, no spinal compression, intragastric administration of physiological saline; other SD rat model with spinal compression was established by the seventh cervical spine intervertebral transplantation, according to average degrees of reformed Rivaling inclined plate test, Tarlov score and BBB scores, the rats were divided into high, moderate and low concentrations groups of YiQi HuoXue prescription, JingFu Kang group and NS group. The rats in high, moderate and low concentrations groups of YiQi HuoXue prescription and Jing Fu Kang group accepted intragastric administration of the corresponding medicine in the ratio of 0.01 m L/g,NS group and sham operation group received the lavage of equivalent dose of NS, medication once each day, for four weeks consecutively. Motor function of the rats were monitored by adopting reformed Rivaling inclined plate test, Tarlov score and BBB scores before intragastric administration, at the first, second, third and forth week after lavage respectively. Results: YiQi HuoXue prescription and Jing Fu Kang in different concentrations could increase average degrees of reformed Rivaling inclined plate test, Tarlov score and BBB scores, the difference had statistical meaning compared with NS group(P<0.05). Conclusion: YiQi HuoXue prescription could promote functional recovery of spinal cord of the rats with chronic spinal compression.
引文
[1]徐远坤.大鼠脊髓型颈椎病动物模型初探[J].中国中医骨伤科杂志,2007:15(3):19-21.
[2]Sharma HS,Badgiyan RD,Alm P,et al.Neuro protective effects of nitroxide synthase inhibitors in spinal cord injury induced pathophysiology and motor func tions:an experimental study in the rat[J].Ann NY Acad Sci,2005,1053:422-434.
[3]夏建龙,杨挺,李松涛.圣愈汤加减对脊髓型颈椎病术后脊髓功能状态的干预[J].中国实验方剂学杂志,2010,16(15):201-204.
[4]陈博来,邓晋丰.活血化瘀中药对大鼠脊髓损伤后血管内皮素及功能恢复的实验研究[J].新中医,2005,37(10):94-96.
[5]任志宏,韦责康,周红海,等.黄芪注射液治疗急性脊髓损伤的实验研究[J].中国实用外科杂志,2009,29(6):103-105.
[6]孙豪栋,庞晓斌,李继扬.黄芪甲苷生物活性研究进展[J].中国药房,2011,22(7):657-661.
[7]陈旭东,赵四敏,华新宇,等.当归对宫内缺氧后新生大鼠大脑CA3区神经元及NMDAR1m RNA表达的影响[J].时珍国医国药,2010,21(9):2182-2183.
[8]韦理,张俐.丹参注射液对急性脊髓损伤大鼠脊髓灰质GDNF m RNA的提高作用及其机制[J].中国中西医结合杂志,2013,33(7):933-937.
[9]Shartna HS.Posttraumatic application of brain derived neurotrophic factor and glia derived neurotrophic factor on the rat spinal cord enhances neuroprotec tion and improves motor function[J].Acta Ncurochir Suppl,2006,96(2):329-334.
[10]孙映君.中药药理学[J].北京:人民卫生出版社,2000:384.
[11]徐孝麟.参麦注射液的临床应用进展[J].中国实用医药,2006,1(4):43-45.
[2]Sharma HS,Badgiyan RD,Alm P,et al.Neuro protective effects of nitroxide synthase inhibitors in spinal cord injury induced pathophysiology and motor func tions:an experimental study in the rat[J].Ann NY Acad Sci,2005,1053:422-434.
[3]夏建龙,杨挺,李松涛.圣愈汤加减对脊髓型颈椎病术后脊髓功能状态的干预[J].中国实验方剂学杂志,2010,16(15):201-204.
[4]陈博来,邓晋丰.活血化瘀中药对大鼠脊髓损伤后血管内皮素及功能恢复的实验研究[J].新中医,2005,37(10):94-96.
[5]任志宏,韦责康,周红海,等.黄芪注射液治疗急性脊髓损伤的实验研究[J].中国实用外科杂志,2009,29(6):103-105.
[6]孙豪栋,庞晓斌,李继扬.黄芪甲苷生物活性研究进展[J].中国药房,2011,22(7):657-661.
[7]陈旭东,赵四敏,华新宇,等.当归对宫内缺氧后新生大鼠大脑CA3区神经元及NMDAR1m RNA表达的影响[J].时珍国医国药,2010,21(9):2182-2183.
[8]韦理,张俐.丹参注射液对急性脊髓损伤大鼠脊髓灰质GDNF m RNA的提高作用及其机制[J].中国中西医结合杂志,2013,33(7):933-937.
[9]Shartna HS.Posttraumatic application of brain derived neurotrophic factor and glia derived neurotrophic factor on the rat spinal cord enhances neuroprotec tion and improves motor function[J].Acta Ncurochir Suppl,2006,96(2):329-334.
[10]孙映君.中药药理学[J].北京:人民卫生出版社,2000:384.
[11]徐孝麟.参麦注射液的临床应用进展[J].中国实用医药,2006,1(4):43-45.