HSPA5和CD44v6在乳腺癌组织中的表达及其诊断价值
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摘要
目的探讨热休克蛋白-5 (HSPA5)和细胞粘附分子CD44V6在乳腺癌组织中的表达及其诊断价值。方法收集上海市长宁区妇幼保健院2014年6月至2016年12月收治的乳腺癌手术患者91例,采用免疫组化二步法对其不同病理组织类型的乳腺癌组织进行HSPA5和CD44V6的表达检测,并对表达结果做统计学分析。结果 HSPA5、细胞黏附分子CD44V6在乳腺癌组织的阳性表达率分别为74.7%(68/91)和62.6%(57/91);不同组织学类型及不同肿瘤直径的乳腺癌组织中HSPA5的阳性表达比较,差异均无统计学意义(P>0.05);而在组织学不同分级和淋巴结有、无转移的乳腺癌组织中,HSPA5的阳性表达比较差异均有显著统计学意义(P<0.01);不同乳腺癌类型、临床分期、不同肿瘤直径及不同组织学分级的乳腺癌组织中,CD44V6的阳性表达比较,差异均无统计学意义(P>0.05),而在肿瘤淋巴结转移阳性组和阴性组之间的阳性表达比较,差异均有显著统计学意义(P<0.01)。结论 HSPA5和CD44V6对于乳腺癌的发生、发展起到重要的调控作用;HSPA5和CD44V6的阳性表达和乳腺癌的生物学行为有关,将HSPA5和CD44V6同时在乳腺癌进行表达分析,对判断乳腺癌的预后和指导其临床治疗有着重要的意义。
Objective To investigate the expression of heat shock protein 5(HSPA5) and CD44 v6 in breast carcinoma and their diagnostic value. Methods A total of 91 cases of patients with breast carcinoma, who admitted to Shanghai Changning Maternity and Infant Health Hospital from June 2014 to December 2016, were examined by immunohistochemical method for HSPA5 and CD44 v6. The expression results were analyzed. Results The positive expression rate of HSPA5 and CD44 v6 in in breast carcinoma were 74.7%(68/91) and 62.6%(57/91) respectively. There was no significant difference in the positive expression of HSPA5 between breast cancer tissues with different histological types and different tumor diameters(P>0.05). However, the positive expression of HSPA5 in breast cancer tissues with or without metastasis of lymph nodes and different histological grades showed significant difference(P<0.01). There was no significant difference in the positive expression of CD44 V6 in breast cancer tissues with different breast cancer types, clinical stages, different tumor diameters and different histological grades(P>0.05). However, there were significant differences in positive expression between positive and negative lymph node metastasis groups(P<0.01). Conclusion HSPA5 and CD44 v6 may play important roles in development of breast cancer. The positive expression of HSPA5 and CD44 V6 is related to the biological behavior of breast cancer. It is important to analyze the expression of HSPA5 and CD44 V6 simultaneously in breast cancer for judging the prognosis of breast cancer and guiding its clinical treatment.
Objective To investigate the expression of heat shock protein 5(HSPA5) and CD44 v6 in breast carcinoma and their diagnostic value. Methods A total of 91 cases of patients with breast carcinoma, who admitted to Shanghai Changning Maternity and Infant Health Hospital from June 2014 to December 2016, were examined by immunohistochemical method for HSPA5 and CD44 v6. The expression results were analyzed. Results The positive expression rate of HSPA5 and CD44 v6 in in breast carcinoma were 74.7%(68/91) and 62.6%(57/91) respectively. There was no significant difference in the positive expression of HSPA5 between breast cancer tissues with different histological types and different tumor diameters(P>0.05). However, the positive expression of HSPA5 in breast cancer tissues with or without metastasis of lymph nodes and different histological grades showed significant difference(P<0.01). There was no significant difference in the positive expression of CD44 V6 in breast cancer tissues with different breast cancer types, clinical stages, different tumor diameters and different histological grades(P>0.05). However, there were significant differences in positive expression between positive and negative lymph node metastasis groups(P<0.01). Conclusion HSPA5 and CD44 v6 may play important roles in development of breast cancer. The positive expression of HSPA5 and CD44 V6 is related to the biological behavior of breast cancer. It is important to analyze the expression of HSPA5 and CD44 V6 simultaneously in breast cancer for judging the prognosis of breast cancer and guiding its clinical treatment.
引文
[1]邱慧兵,王越华,易铁男.双侧乳腺癌40例组织学分析[J].肿瘤研究与临床, 2015, 27(12):827-830.
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[4] TAVASSOLI FA, DEVILEE P. WHO classification tumors of thebreast and female genital organs[M]. Lyon:IRAC Press, 2003:69-103.
[5]内藤善哉,乔炎,浅野伍朗.热休克蛋白在人乳腺癌中的表达[J].中华病理学杂志, 1998, 27(1):35-39.
[6]刘涛,田聆,魏于全,等.热休克蛋白与肿瘤的研究进展[J].华西医学, 2004, 19(3):495-497.
[7]王文亭,李建远. HSPA5研究进展[J].中外医学研究, 2010, 8(8):26-27.
[8] ORTIZ C, CARDEMIL L. Heat-shock responses in two leguminousplants:a comparative study[J]. Exp Bot, 2001, 52(361):1711-1719.
[9] LEE AS. The glucose-regulated proteins:stress in duction an clinicalapplications[J]. Trends Biochem Sci, 2001, 26(8):504-510.
[10] YANG GH, LI S, PESTKA JJ. Down-regulation of endoplasmicretic-ulum chaperone GRP78/Bip by vomitoxin(Deoxynivalenol)[J]. Tox-icol Appl harmacol, 2000, 162(3):207-217.
[11]毕爱华.医学免疫学[M].北京:人民军医出版社, 1995:87-89.
[12]房新建,张磊,孟祥启,等. CD44st基因在非小细胞肺癌组织中的表达及其意义[J].肿瘤研究与临床, 2015, 27(4):238-242.
[13]李伟东,刘芳芳,徐新生,等.乳腺浸润性乳头状癌中CD44+/CD24(-/low)和CD24+表型细胞的研究[J].中华乳腺病杂志(电子版),2009, 3(5):35-41.
[14]廖治,肖洪涛,瞿大成,等. CD44分子与子宫内膜癌关系的研究进展[J].西部医学, 2009, 21(6):1027-1028.
[2] URAMOTO H, SUGIO K, OYAMA T, et al. Expression of endoplas-mic reticulum molecular chaperone Grp78 in human lung cancer andits clinical significance[J]. Lung Cancer, 2005, 49(1):55-62.
[3] STANG K, GUNTHER C, FRANK T, et al. Inhibition of the ubiqui-tin-proteosome pathway induces differential heat-shock protein re-sponse in cardiomyocytes and renders early cardiac protection[J].Biochem Biophys Res Commun, 2002, 291(3):542-549.
[4] TAVASSOLI FA, DEVILEE P. WHO classification tumors of thebreast and female genital organs[M]. Lyon:IRAC Press, 2003:69-103.
[5]内藤善哉,乔炎,浅野伍朗.热休克蛋白在人乳腺癌中的表达[J].中华病理学杂志, 1998, 27(1):35-39.
[6]刘涛,田聆,魏于全,等.热休克蛋白与肿瘤的研究进展[J].华西医学, 2004, 19(3):495-497.
[7]王文亭,李建远. HSPA5研究进展[J].中外医学研究, 2010, 8(8):26-27.
[8] ORTIZ C, CARDEMIL L. Heat-shock responses in two leguminousplants:a comparative study[J]. Exp Bot, 2001, 52(361):1711-1719.
[9] LEE AS. The glucose-regulated proteins:stress in duction an clinicalapplications[J]. Trends Biochem Sci, 2001, 26(8):504-510.
[10] YANG GH, LI S, PESTKA JJ. Down-regulation of endoplasmicretic-ulum chaperone GRP78/Bip by vomitoxin(Deoxynivalenol)[J]. Tox-icol Appl harmacol, 2000, 162(3):207-217.
[11]毕爱华.医学免疫学[M].北京:人民军医出版社, 1995:87-89.
[12]房新建,张磊,孟祥启,等. CD44st基因在非小细胞肺癌组织中的表达及其意义[J].肿瘤研究与临床, 2015, 27(4):238-242.
[13]李伟东,刘芳芳,徐新生,等.乳腺浸润性乳头状癌中CD44+/CD24(-/low)和CD24+表型细胞的研究[J].中华乳腺病杂志(电子版),2009, 3(5):35-41.
[14]廖治,肖洪涛,瞿大成,等. CD44分子与子宫内膜癌关系的研究进展[J].西部医学, 2009, 21(6):1027-1028.