水稻转绿型新叶黄化不育系热农1A的光合特性研究（英文）
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摘要
[目的]了解水稻热农1A的光合特性可促进其在育种上的利用。[方法]该研究以热农1A的父本新叶黄化突变体对应的野生型WT为对照,在分蘖期测定光合色素含量、光合参数、光响应曲线和叶绿素荧光动力学参数。[结果]热农1A的新生叶前期黄化明显,其叶绿素含量、光合速率、P_n、F_m、F_v’/F_m’、Φ_(PSII)和ETR均显著低于对照,而叶片转绿后的光合速率与对照相比无差异。[结论]热农1A新生叶前期因降低的叶绿素含量导致其光合能力减弱,而后期随着叶绿素含量提高,其光合作用能力恢复正常。
[Objective] Understanding the photosynthetic characteristics of the rice CMS line Renong 1A with leaf color markers can promote its utilization in breeding.[Method] The wild type(WT) of rice spontaneous mutant with virescent-yellow leaves that was the male parent of Renong 1A was selected as the control, and the contents of photosynthetic pigments, photosynthetic parameters, light-response curve and chlorophyll fluorescence kinetic parameters of Renong 1A were measured at the tillering stage. [Result] New leaves of Renong 1A yellowed obviously in the early period. Chlorophyll content, photosynthetic rate and values of parameters P_n,F_v/F_m, F_v'/F_m', Φ_(PSII) and ETR of Renong 1A were all significantly lower than those of the control. However, there was no difference between Renong 1A and the control in terms of photosynthetic rate of leaves turning green. [Conclusion] The photosynthetic capacity of new leaves of Renong 1A decreased at the early stage due to the reduction of chlorophyll content, and then it became normal at the later stage with the increase of chlorophyll content.
[Objective] Understanding the photosynthetic characteristics of the rice CMS line Renong 1A with leaf color markers can promote its utilization in breeding.[Method] The wild type(WT) of rice spontaneous mutant with virescent-yellow leaves that was the male parent of Renong 1A was selected as the control, and the contents of photosynthetic pigments, photosynthetic parameters, light-response curve and chlorophyll fluorescence kinetic parameters of Renong 1A were measured at the tillering stage. [Result] New leaves of Renong 1A yellowed obviously in the early period. Chlorophyll content, photosynthetic rate and values of parameters P_n,F_v/F_m, F_v'/F_m', Φ_(PSII) and ETR of Renong 1A were all significantly lower than those of the control. However, there was no difference between Renong 1A and the control in terms of photosynthetic rate of leaves turning green. [Conclusion] The photosynthetic capacity of new leaves of Renong 1A decreased at the early stage due to the reduction of chlorophyll content, and then it became normal at the later stage with the increase of chlorophyll content.
引文
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[13]HE ZZ(贺治洲),YIN M(尹明),XIE ZY(谢振宇),et al.Comparative studies on characteristics of rice CMS line with yellow green-revertible leaf(水稻转绿型新叶黄化不育系特征特性对比研究)[J].Seed(种子),2014,33(11):1-4.
[14]TANG ZY,WAN GQ,LIU T,et al.Chlorophyll fluorescence light kinetics parameters to main cotton varieties in North Xinjiang[J].Acta Gossypii Sinica,2004,16(3):166-169.
[15]LI ZB,WEI YN,ZHANG RH,et al.Primary studies on chlorophyll fluorescence characteristics of cotton leaves at different leaf position[J].Acta Gossypii Sinica,2005,17(3):189-190.
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[17]ROHCEK K,BARTK M.Technique of the modulated chlorophyll fluorescence:basis concepts,useful parameters and some applications[J].Photosynthetica,1999,37:339-363.
[2]SU N,HU M,WU DX,et al.Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production[J].Plant Physiology,2012,159(1):227-238.
[3]SHU QY(舒庆尧),CHEN SF(陈善福),WU DX(吴殿星),et al.Studies on breeding of a new type of cytoplasmic male sterile rice line Quanlong A(新型不育系全龙A的选育与研究)[J].Scientia Agricultura Sinica(中国农业科学),2001,34(4):349-354.
[4]SHEN SQ(沈圣泉),SHU QY(舒庆尧),WU DX(吴殿星),et al.Breeding of a new rice CMS line Baifeng A with a green-revertible albino leaf color marker(白化转绿型水稻三系不育系白丰A的选育)[J].Hybrid Rice(杂交水稻),2005,20(5):10-11.
[5]LI XL(李小林),DENG AF(邓安凤),DENG JL(邓君浪),et al.Breeding of a sterile line Yunfeng 66A with greenrevertible albino leaf-color markers in rice(水稻苗期白化转绿型叶色标记籼型不育系云丰66A的选育)[J].Southwest China Journal of Agricultural Sciences(西南农业学报),2011,24(2):410-413.
[6]LI XL(李小林),DENG AF(邓安凤),XUYR(徐雨然),et al.Breeding of rice leaf margin albino leaf-color marker three sterile line Yunfeng 88 A(水稻叶缘白化叶色标记三系不育系云丰88A的选育)[J].Seed(种子),2011,30(12):109-111.
[7]ZHAO HJ(赵海军),WU DX(吴殿星),SHU QY(舒庆尧),et al.Breeding and characteristics of photo-thermo sensitive genic male sterile rice Yutu S labeled with green-revertible albino leaf marker(携带白化转绿型叶色标记光温敏核不育系玉兔S的选育及其特征特性)[J].Chinese Journal of Rice Science(中国水稻科学),2004,18(6):515-521.
[8]LU HJ(卢华金),YANG WQ(杨文清),LOU Y(楼珏),et al.Breeding of Nanfeng 8S,a PTGMS line with a marker of green-revertible albino leaves in seedling stage in rice(幼苗期带白化转绿叶色标记的水稻光温敏核不育系楠丰8S的选育)[J].Hybrid Rice(杂交水稻),2012,27(1):16-18.
[9]WU W(吴伟),LIU X(刘鑫),SHU XL(舒小丽),et al.Two-line hybrid rice male sterile line'NHR111S'with a marker of green-revertible albino leaves(携带白化转绿型叶色标记两系杂交水稻不育系NHR111S)[J].Journal of Nuclear Agricultural Sciences(核农学报),2006,20(2):103-105.
[10]HE ZZ(贺治洲),YIN M(尹明),XIE ZY(谢振宇),et al.Research advances on green revertible leaf-color mutants in rice(水稻转绿型叶色突变体研究进展)[J].Chinese Journal of Tropical Agriculture(热带农业科学),2014(8):30-36.
[11]HE ZZ(贺治洲),YIN M(尹明),XIE ZY(谢振宇),et al.Breeding of indica CMSline Renong 1A with yellow greenrevertible leaf in rice(转绿型新叶黄化籼稻不育系热农1A的选育)[J].Hybrid Rice(杂交水稻),2014(5):7-9.
[12]LI JQ,WANG YH,CHAI JT,et al.Green-revertible Chlorina 1(grc1)is required for the biosynthesis of chlorophyll and the early development of chloroplasts in rice[J].Journal of Plant Biology,2013,56(5):326-335.
[13]HE ZZ(贺治洲),YIN M(尹明),XIE ZY(谢振宇),et al.Comparative studies on characteristics of rice CMS line with yellow green-revertible leaf(水稻转绿型新叶黄化不育系特征特性对比研究)[J].Seed(种子),2014,33(11):1-4.
[14]TANG ZY,WAN GQ,LIU T,et al.Chlorophyll fluorescence light kinetics parameters to main cotton varieties in North Xinjiang[J].Acta Gossypii Sinica,2004,16(3):166-169.
[15]LI ZB,WEI YN,ZHANG RH,et al.Primary studies on chlorophyll fluorescence characteristics of cotton leaves at different leaf position[J].Acta Gossypii Sinica,2005,17(3):189-190.
[16]OXBOROUGH K,BAKER NR.Resolving chlorophyll a flurescence images of photosynthetic efficiency into photochemical and non-photochemical components-calculation of q P and Fv/Fm without measuring Fo[J].Photosynthesis Research,1997,54(2):135-142.
[17]ROHCEK K,BARTK M.Technique of the modulated chlorophyll fluorescence:basis concepts,useful parameters and some applications[J].Photosynthetica,1999,37:339-363.