微卫星不稳定、血管内皮生长因子表达情况与大肠癌患者病理特征及预后相关性分析
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摘要
目的探讨分析微卫星不稳定(MSI)、血管内皮生长因子(VEGF)表达情况与大肠癌患者病理特征及预后相关性。方法回顾性分析本院107例行大肠癌根治术治疗的大肠癌患者临床病理资料,采用免疫组织化学方法检测107例患者手术标本肿瘤组织内MSI及VEGF表达情况,并比较MSI、VEGF表达与患者病理特征、预后相关性。结果 MSI与患者淋巴结转移与浸润深度相关(P<0.05);VEGF表达与患者血行转移、淋巴结转移、TNM分期及浸润深度相关(P<0.05);高微卫星不稳定性(MSI-H)大肠癌VEGF的表达显著减少。结论MSI与大肠癌患者淋巴结转移与浸润深度相关,而VEGF表达与大肠癌患者血行转移、淋巴结转移、TNM分期与浸润深度相关,MSI-H患者其生存及预后情况显著优于MSS及MSI-L患者;VEGF阴性患者其生存情况显著优于VEGF阳性患者。MSI-H大肠癌与微卫星稳定性(MSS)大肠癌可能存在两种不同的新生血管形成的途径,MSI-H肿瘤较低的VEGF表达或许可解释为何MSI-H大肠癌有较低的侵袭性。
Objective To investigate the correlation between microsatellite instability,vascular endothelial growth factor expression and pathological characteristics and prognosis of colorectal cancer patients. Methods In our hospital, 107 cases with colorectal cancer treated with radical surgery were retrospective studied. Clinical and pathological data were collected, and the MSI and VEGF expression in the surgical specimens were detected by immunohistochemical method. The correlation between MSI and VEGF expression and clinic pathological features and prognosis was compared. Results The expression of MSI was correlated with lymph node metastasis and invasion depth(P<0.05). The expression of VEGF was correlated with hematogenous metastasis, lymph node metastasis, TNM staging and the depth of tumor invasion( P<0.05). The survival time of MSS patients was significantly better than that of the MSI patients(P<0.05). The survival rate of VEGF negative patients was significantly higher than that of VEGF positive patients(P<0.05). Conclusion MSI and colorectal cancer patients with lymph node metastasis and invasion depth, and VEGF expression and blood metastasis in patients with colorectal cancer, lymph node metastasis, TNM staging and depth of invasion, MSI-H patient survival and prognosis was significantly better than the MSS and MSI-L patients; VEGF-negative patients whose survival was significantly better than VEGFpositive patients. MSI-H colorectal cancer with microsatellite stable(MSS) colorectal cancer may exist in two different ways neovascularization lower MSI-H tumors VEGF expression perhaps explain why MSI-H colorectal cancer have a lower invasion sex.
Objective To investigate the correlation between microsatellite instability,vascular endothelial growth factor expression and pathological characteristics and prognosis of colorectal cancer patients. Methods In our hospital, 107 cases with colorectal cancer treated with radical surgery were retrospective studied. Clinical and pathological data were collected, and the MSI and VEGF expression in the surgical specimens were detected by immunohistochemical method. The correlation between MSI and VEGF expression and clinic pathological features and prognosis was compared. Results The expression of MSI was correlated with lymph node metastasis and invasion depth(P<0.05). The expression of VEGF was correlated with hematogenous metastasis, lymph node metastasis, TNM staging and the depth of tumor invasion( P<0.05). The survival time of MSS patients was significantly better than that of the MSI patients(P<0.05). The survival rate of VEGF negative patients was significantly higher than that of VEGF positive patients(P<0.05). Conclusion MSI and colorectal cancer patients with lymph node metastasis and invasion depth, and VEGF expression and blood metastasis in patients with colorectal cancer, lymph node metastasis, TNM staging and depth of invasion, MSI-H patient survival and prognosis was significantly better than the MSS and MSI-L patients; VEGF-negative patients whose survival was significantly better than VEGFpositive patients. MSI-H colorectal cancer with microsatellite stable(MSS) colorectal cancer may exist in two different ways neovascularization lower MSI-H tumors VEGF expression perhaps explain why MSI-H colorectal cancer have a lower invasion sex.
引文
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14 樊善继,徐海帆,胡泽成,等.大肠癌患者组织多项基质金属蛋白、VEGF-C、VEGF-D、Ets-1、CD44v6及VEGFR-3的变化[J].海南医学院学报,2014,20(2):177-179,183.
15 王前,安泽武.HIF-1α和VEGF在大肠癌中的表达及其相关性研究[J].肿瘤学杂志,2013,19(11):858-861.
16 Xing X,Gu X,Ma T,et al.Biglycan up-regulated vascular endothelial growth factor(VEGF)expression and promoted angiogenesis in colon cancer[J].Tumour Biol,2015,36(3):1773-1780.
2 Furuhata T,Okita K,Nishidate T,et al.Oncological benefit of primary tumor resection with high tie lymph node dissection in unresectable colorectal cancer with synchronous peritoneal metastasis:a propensity score analysis of data from a multi-institute database[J].Int J Clin Oncol,2015,20(5):922-927.
3 王炜东,袁玉松,何嗪,等.结肠癌发生、发展与mi RNA分子机制相关研究进展[J].中国生化药物杂志,2015,7(7):169-171.
4 黄波,李伟明,冯智毅,等.转化生长因子β受体Ⅱ对微卫星不稳定结肠癌细胞凋亡和迁移的影响[J].中华生物医学工程杂志,2012,18(2):107-111.
5 端传友.MMP-2、MMP-9及VEGF在结肠癌中的表达及其与肿瘤转移的相关性[J].中国医药导报,2012,9(21):44-45,48.
6 Yang L,Sun Y,Huang XE,et al.Carcinoma microsatellite instability status as a predictor of benefit from fluorouracil-based adjuvant chemotherapy for stageⅡrectal cancer[J].Asian Pac J Cancer Prev,2015,16(4):1545-1551.
7 吴静,龚旭晨,孔长青,等.新疆维吾尔族MT1XT20基因与微卫星不稳定结直肠癌相关性研究[J].安徽医科大学学报,2015,(6):867-869.
8 张剑权,符国珍,吴海红,等.遗传性非息肉病性大肠癌微卫星不稳定临床病理和错配修复基因变异的研究[J].中华实验外科杂志,2014,31(2):245-247.
9 孟文建,王玲,于永扬,等.微卫星不稳定在散发性Ⅱ、Ⅲ期直肠癌中的意义[J].重庆医学,2010,39(18):2420-2422,2426.
10 王小峰,金黑鹰,丁义江,等.微卫星不稳定对散发性结直肠癌患者预后的影响[J].中华胃肠外科杂志,2011,14(7):520-523.
11 韩晶.结直肠癌微卫星不稳定(MSI)与其发病及预后的关系[J].中国肿瘤临床,2012,39(1):56-59
12 Lee DH,Lee JH,Kim DK,et al.Nuclear and mitochondrial DNAs microsatellite instability and mitochondrial DNA copy number in adenocarcinoma and squamous cell carcinoma of lung:a pilot study[J].APMIS,2015,123(12):1048-1054.
13 邢恩鸿,王军,王春阳,等.大肠癌组织PTTG、VEGF-C表达及其与微淋巴管生成的关系[J].山东医药,2013,53(6):25-28.
14 樊善继,徐海帆,胡泽成,等.大肠癌患者组织多项基质金属蛋白、VEGF-C、VEGF-D、Ets-1、CD44v6及VEGFR-3的变化[J].海南医学院学报,2014,20(2):177-179,183.
15 王前,安泽武.HIF-1α和VEGF在大肠癌中的表达及其相关性研究[J].肿瘤学杂志,2013,19(11):858-861.
16 Xing X,Gu X,Ma T,et al.Biglycan up-regulated vascular endothelial growth factor(VEGF)expression and promoted angiogenesis in colon cancer[J].Tumour Biol,2015,36(3):1773-1780.