响应面法优化制备马铃薯微孔淀粉的研究
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摘要
以马铃薯为实验材料制备微孔淀粉,考察复合酶添加量及复合酶中α-淀粉酶和糖化酶的比例、反应温度、反应时间和反应pH对微孔淀粉吸油率、吸水率的影响。根据单因素实验结果,采用Box-Behnken试验设计和响应面分析法,确定其最佳工艺条件为:加酶量0.85%、α-淀粉酶和糖化酶的比例1∶2,温度50℃,反应时间12h,pH 4.15,此条件下微孔马铃薯淀粉得率为61.39%,淀粉的吸油率为66.85%。回归模型吸油率的预测值与实测值接近,表明响应面法对马铃薯微孔淀粉制备工艺的优化合理可行。
To obtain potato microporous starch,the effects of the amount of complex enzyme and ratio of α-amylase and Amyloglucosidase in the complex enzyme,reaction temperature,reaction time and reaction pH on oil absorption rate and water absorption of microporous starch were investigated. Based on the results of single factor experiments,Box-Behnken test was designed and response surface analysis method was applied to determine the optimal condition,which was :enzyme complex 0.85 %,ratio of α-Amylase and Amyloglucosidase 1 ∶ 2,temperature 50 ℃,reaction time 12 h and pH 4.15. Under these conditions,the yield of microporous potato starch was 61.39%,and oil absorption rate of starch was 66.85%. The predicted value of the regression model is close to the testing value,indicating that the response surface method is reasonable and feasible for optimization of potato microporous starch preparation process.
To obtain potato microporous starch,the effects of the amount of complex enzyme and ratio of α-amylase and Amyloglucosidase in the complex enzyme,reaction temperature,reaction time and reaction pH on oil absorption rate and water absorption of microporous starch were investigated. Based on the results of single factor experiments,Box-Behnken test was designed and response surface analysis method was applied to determine the optimal condition,which was :enzyme complex 0.85 %,ratio of α-Amylase and Amyloglucosidase 1 ∶ 2,temperature 50 ℃,reaction time 12 h and pH 4.15. Under these conditions,the yield of microporous potato starch was 61.39%,and oil absorption rate of starch was 66.85%. The predicted value of the regression model is close to the testing value,indicating that the response surface method is reasonable and feasible for optimization of potato microporous starch preparation process.
引文
[1]马玉荣.鲜切马铃薯褐变控制技术研究[D].山东农业大学,2010.
[2]余欣荣.马铃薯将成我国第四大主粮[J].农业机械,2015(1):29-29.
[3]郝智勇.马铃薯加工产业存在问题及发展建议[J].黑龙江农业科学,2017(7):89-91.
[4]Zhou J,Wang Y H,Chu J,et al.Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments.[J].Bioresource Technology,2009,100(2):819-25.
[5]Yang L,Ning X,Chen K,et al.Preparation and properties of hydroxyapatite filters for microbial filtration[J].Ceramics International,2007,33(3):483-489.
[6]Sopyan I,Mel M,Ramesh S,et al.Porous hydroxyapatite for artificial bone applications[J].Science&Technology of Advanced Materials,2007,8(1):116-123.
[7]许丽娜,董海洲,张绪霞,等.多孔淀粉制备及开发前景[J].粮食与油脂,2007(2):18-20.
[8]Tester R F,Qi X,Karkalas J.Hydrolysis of native starches with amylases[J].Animal Feed Science&Technology,2006,130(2):39-54.琶洲新馆网址:www.cfaa.cn展出时间:2018年1八主办单位:中国食品添加剂和配料协会北京中食添会展中心秋季全国FIC-Autu展出地点:广州中国
[9]杨圣岽,侯聚敏,张琳,等.酶法制备微孔淀粉的研究进展[J].东北农业科学,2012,37(4):77-80.
[10]李芳良,麻昌爱,韦群兰,等.复合酶解法制备木薯微孔淀粉的研究[J].农产品加工:学刊,2009(7):55-56.
[11]张欢欢,郅琦.双酶法制备板栗微孔淀粉的工艺研究[J].食品工业,2017(2):81-84.
[12]张超.玉米多孔淀粉的酶法制备及其作为吸附剂的应用研究[D].江南大学,2012.
[13]李欣欣,杨圣岽,吴海成,等.复合酶法制备微孔淀粉工艺条件的优化[J].安徽农业科学,2012(12):7438-7439.
[14]姚卫蓉,姚惠源.多孔淀粉的研究[J].中国粮油学报,2001,16(1):36-39.
[15]Shi Yu-ting,Wang Zhi-geng,FangYu-ming,et al.Study on Dual-enzyme Hydrolysisβ-Lactoglobulin[J].Food and Fermentation Industries,2010.
[16]Wang Yugao,Zhang Liming,Li Xinglin,et al.Physicochemical properties of starches from two different yam(Dioscorea opposita Thunb.)residues[J].Brazilian Archives of Biology&Technology,2011,54(2):243-251.
[17]王芳.以淀粉为基质的EGCG载体化构建及特性研究[D].江南大学,2016.电话:010-597958331月8日~10日《中国食品添加剂》杂志社有限公司mn 2018进出口商品交易会展馆
[2]余欣荣.马铃薯将成我国第四大主粮[J].农业机械,2015(1):29-29.
[3]郝智勇.马铃薯加工产业存在问题及发展建议[J].黑龙江农业科学,2017(7):89-91.
[4]Zhou J,Wang Y H,Chu J,et al.Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments.[J].Bioresource Technology,2009,100(2):819-25.
[5]Yang L,Ning X,Chen K,et al.Preparation and properties of hydroxyapatite filters for microbial filtration[J].Ceramics International,2007,33(3):483-489.
[6]Sopyan I,Mel M,Ramesh S,et al.Porous hydroxyapatite for artificial bone applications[J].Science&Technology of Advanced Materials,2007,8(1):116-123.
[7]许丽娜,董海洲,张绪霞,等.多孔淀粉制备及开发前景[J].粮食与油脂,2007(2):18-20.
[8]Tester R F,Qi X,Karkalas J.Hydrolysis of native starches with amylases[J].Animal Feed Science&Technology,2006,130(2):39-54.琶洲新馆网址:www.cfaa.cn展出时间:2018年1八主办单位:中国食品添加剂和配料协会北京中食添会展中心秋季全国FIC-Autu展出地点:广州中国
[9]杨圣岽,侯聚敏,张琳,等.酶法制备微孔淀粉的研究进展[J].东北农业科学,2012,37(4):77-80.
[10]李芳良,麻昌爱,韦群兰,等.复合酶解法制备木薯微孔淀粉的研究[J].农产品加工:学刊,2009(7):55-56.
[11]张欢欢,郅琦.双酶法制备板栗微孔淀粉的工艺研究[J].食品工业,2017(2):81-84.
[12]张超.玉米多孔淀粉的酶法制备及其作为吸附剂的应用研究[D].江南大学,2012.
[13]李欣欣,杨圣岽,吴海成,等.复合酶法制备微孔淀粉工艺条件的优化[J].安徽农业科学,2012(12):7438-7439.
[14]姚卫蓉,姚惠源.多孔淀粉的研究[J].中国粮油学报,2001,16(1):36-39.
[15]Shi Yu-ting,Wang Zhi-geng,FangYu-ming,et al.Study on Dual-enzyme Hydrolysisβ-Lactoglobulin[J].Food and Fermentation Industries,2010.
[16]Wang Yugao,Zhang Liming,Li Xinglin,et al.Physicochemical properties of starches from two different yam(Dioscorea opposita Thunb.)residues[J].Brazilian Archives of Biology&Technology,2011,54(2):243-251.
[17]王芳.以淀粉为基质的EGCG载体化构建及特性研究[D].江南大学,2016.电话:010-597958331月8日~10日《中国食品添加剂》杂志社有限公司mn 2018进出口商品交易会展馆