脾阳虚大鼠腹腔消化吸收结构表面微循环血流量变化及其机制研究
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摘要
目的研究脾阳虚模型大鼠腹腔主要消化吸收结构表面微循环血流量的变化,并从血栓素A_2(TXA_2)/前列环素2(PGI_2)(T/P)比值方面探讨其发生机制。方法选取16只SPF级雄性SD大鼠,随机分为对照组和脾阳虚组(模型组),每组8只。采用激光散斑技术检测大鼠肝、胰腺、胃、小肠和小肠系膜等表面微循环的实时血流量,采用ELISA法检测血清TXA_2和PGI_2水平。结果与对照组相比,模型组大鼠肝左叶、肝右侧叶、胰腺、胃和小肠的血流量及T/P比值均显著增加(均P<0.01),但小肠系膜血流量和血清PGI_2浓度却明显减少(均P<0.01)。结论脾阳虚时腹腔主要消化结构存在血流增加现象,其机制与T/P比值升高有关。
Objective To study blood flow changes of main visceral organs of digestion and absorption in spleen yang deficiency and its possible mechanism from the ratio of thromboxane A_2(TXA_2) and prostacyclin(PGI_2).Methods 16 SPF rats were randomly divided into the control and the spleen yangdeficiency group(the model group),8 rats in each group.Laser speckle was used to detect blood flow changes in liver,pancreas,stomach,small intestine(SI) and its mesenterium.ELISA was employed to measure serum thromboxane A_2(TXA_2) and prostacyclin(PGI_2) concentrations.Results Compared with those in the control group,blood flows inliver left and right lobes,pancreas,stomach and small intestine and TXA_2/PGI_2(T/A) value were significantly increased in the model group(all P<0.01),but blood flow of SI mesenterium and serum PGI_2 level were decreased obviously in the model group(all P<0.01).Conclusion There is enhancement of blood flows in main visceral organs of digestion and absorption in spleen yang deficiency,and its underlying mechanism might be attributed to the increase of T/P value.
Objective To study blood flow changes of main visceral organs of digestion and absorption in spleen yang deficiency and its possible mechanism from the ratio of thromboxane A_2(TXA_2) and prostacyclin(PGI_2).Methods 16 SPF rats were randomly divided into the control and the spleen yangdeficiency group(the model group),8 rats in each group.Laser speckle was used to detect blood flow changes in liver,pancreas,stomach,small intestine(SI) and its mesenterium.ELISA was employed to measure serum thromboxane A_2(TXA_2) and prostacyclin(PGI_2) concentrations.Results Compared with those in the control group,blood flows inliver left and right lobes,pancreas,stomach and small intestine and TXA_2/PGI_2(T/A) value were significantly increased in the model group(all P<0.01),but blood flow of SI mesenterium and serum PGI_2 level were decreased obviously in the model group(all P<0.01).Conclusion There is enhancement of blood flows in main visceral organs of digestion and absorption in spleen yang deficiency,and its underlying mechanism might be attributed to the increase of T/P value.
引文
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[23]Wiktorowska-Owczarek A,Berezińska M,Nowak JZ.PUFAs:structures,metabolism and functions[J].Adv Clin Exp Med,2015,24(6):931-941.
[2]刘旭东,刘文俊,孙大宇,等.脾气虚证模型大鼠神疲乏力的客观化评价[J].中华中医药杂志,2015,30(3):699-701.
[3]刘洋,刘旭东,刘文俊,等.基于脾气虚的脾阳虚大鼠模型的复制方法及评价标准研究[J].中国医学创新,2016,13(3):25-28.
[4]王晓明,易杰,廖世新,等.脾虚证动物模型的客观评估[J].中华中医药杂志,2006,21(7):406-408.
[5]王颖,郑小伟,李秀月.模拟中医病因所致脾气虚、脾阳虚大鼠模型的证候比较研究[J].中华中医药学刊,2012,30(1):104-107.
[6]唐汉庆,卢兰,李晓华,等.附子注射液对冠心病心脾阳虚证模型大鼠离体心肌的影响[J].中国中医基础医学杂志,2015,21(8):933-935,976.
[7]梁忠,黄波,陈军.中医之经气与氧代谢相关性研究[J].中国针灸,2012,32(2):183-186.
[8]张海生.揭示中医学说中“气”概念的本质——古代文献的理解及生理学的事实[J].光明中医,2013,28(12):2470-2481.
[9]刘艳丽,王秀秀,韩金祥.中医“气”学说研究60年[J].辽宁中医杂志,2014,41(11):2299-2303.
[10]张启明,张毅,解君,等.中医五藏系统的功能定位[J].北京中医药大学学报,2014,37(7):437-442.
[11]章莹,吴承玉.中医脾系病位特征及脾系相关疾病之初探[J].江苏中医药,2011,43(2):6-7.
[12]唐元瑜,纪立金,王尔宁.从中医脾的实体解剖学研究探微脾主运化功能[J].浙江中医药大学学报,2011,35(6):821-823,833.
[13]何劲,周莉,陈家旭,等.中医脾虚证实质研究进展[J].吉林中医药,2015,35(1):103-107.
[14]冷雪,张立德,贾连群,等.人参皂苷Rb1对异丙肾上腺素诱导大鼠急性心肌缺血影响的作用机制[J].中国实验方剂学杂志,2015,21(24):104-108.
[15]李迎红,李敏,刘崴,等.从皮肤微循环血流灌注量看身柱穴特异性[J].上海针灸杂志,2016,35(2):199-201.
[16]周陈陈,石玉婷,于世家,等.补肾活血中药对实验性糖尿病大鼠肾脏皮层血流值影响的观察[J].中华中医药学刊,2016,34(3):712-714.
[17]臧安缘,贾连群,冷雪,等.电针刺激合用人参皂苷素Rb1对大鼠急性心肌缺血血流及血清酶学的影响[J].吉林中医药,2016,36(10):1029-1032.
[18]魏锡文,郑曙琴.利用激光散斑成像技术观察2型糖尿病合并动脉硬化症患者舌象微循环变化的临床研究[J].辽宁医学杂志,2015,29(4):214-215.
[19]丁连翠,路新国.药膳“益脾饮”对脾气虚证大鼠血液流变学及血浆TXB2、6-keto-PGF1α的影响[J].西部中医药,2012,25(5):33-35.
[20]沙盈盈,梁超,朱晓明,等.活血清解汤早期干预对重症急性胰腺炎患者血液流变学及D二聚体、血栓素A2、前列环素Ⅰ2的影响[J].临床肝胆病杂志,2016,32(4):678-681.
[21]康群甫,刘卫红,任攀,等.活血解毒中药对动脉粥样硬化小鼠血清PGI2/TXA2及DNA甲基化水平的干预作用[J].北京中医药,2016,35(4):309-314.
[22]Kasza Z,Fetalvero KM,Ding M,et al.Novel signaling pathways promote a paracrine wave of prostacyclin-induced vascular smooth muscle differentiation[J].J Mol Cell Cardiol,2009,46(5):682-694.
[23]Wiktorowska-Owczarek A,Berezińska M,Nowak JZ.PUFAs:structures,metabolism and functions[J].Adv Clin Exp Med,2015,24(6):931-941.