电针足三里穴对FGIDs大鼠胃肠微循环及5-HT影响研究
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摘要
目的:观察电针足三里穴对功能性胃肠病(FGIDs)模型大鼠胃、肠表面微循环以及五羟色胺(5-HT)含量的影响,并探讨其作用机制。方法:综合方法复制FGIDs大鼠模型。电针足三里穴,并以阴谷穴和非经非穴为对照,每天1次,共针刺7 d,激光多普勒血流仪同步监测胃、肠表面微循环血流量,采用酶联免疫法检测血、下丘脑、脊髓、胃、肠中5-HT含量的变化。结果:电针足三里穴可以明显改善胃、肠表面微循环,增加其微循环血流量(P<0.05),并可以明显降低FGIDs模型大鼠异常升高的血、下丘脑、脊髓及胃肠等各部位5-HT含量(P<0.05),且与阴谷穴、非经非穴相比,胃肠表面微循环血流量和各部位5-HT含量的变化皆有显著性差异(P<0.05)。结论:电针足三里穴可以改善FGIDs模型大鼠胃肠微循环状况而改善其症状,可能机制与整体调节各水平部位的5-HT含量有关,而且具有穴位特异性。
Objective:To observe the effect of electroacupuncture(EA)at Zusanli(ST36)on microcirculation of gastric and intestinal surface and the content of 5-HT in the functional gastrointestinal disorders(FGIDs)model rats,and investigate its mechanism. Methods:The FGIDs rat model was established by comprehensive method. EA at Zusanli(ST 36)of FGIDs model rats,and contrast with Yingu(KI10)point and non-meridian-non-acupoint. once a day,a total of 7 days of acupuncture. Simultaneously,the gastric and intestinal surface microcirculation blood flow was monitored by Laser Doppler flowmetry,and the change of 5-HT content in blood,hypothalamus,spinal cord,the stomach and intestines was detected through enzyme-linked immunosorbentassay. Results:The gastrointestinal microcirculation was obviously improved in Zusanli group(P<0.05),and the content of 5-HT was reduced in blood,hypothalamus,spinal cord,the stomach and intestines and so on all positions of the FGIDs model rats(P<0.05),and compared with the Yingu(KI10)group and the non-meridian-non-acupoint group,there was significant difference(P<0.05). Conclusion:FGIDs model rats' gastrointestinal microcirculation status on the whole can be improved by EA at Zusanli(ST36),and the possible mechanism is related to the 5-HT content of all horizontal positions,and there is acupoint specificity.
Objective:To observe the effect of electroacupuncture(EA)at Zusanli(ST36)on microcirculation of gastric and intestinal surface and the content of 5-HT in the functional gastrointestinal disorders(FGIDs)model rats,and investigate its mechanism. Methods:The FGIDs rat model was established by comprehensive method. EA at Zusanli(ST 36)of FGIDs model rats,and contrast with Yingu(KI10)point and non-meridian-non-acupoint. once a day,a total of 7 days of acupuncture. Simultaneously,the gastric and intestinal surface microcirculation blood flow was monitored by Laser Doppler flowmetry,and the change of 5-HT content in blood,hypothalamus,spinal cord,the stomach and intestines was detected through enzyme-linked immunosorbentassay. Results:The gastrointestinal microcirculation was obviously improved in Zusanli group(P<0.05),and the content of 5-HT was reduced in blood,hypothalamus,spinal cord,the stomach and intestines and so on all positions of the FGIDs model rats(P<0.05),and compared with the Yingu(KI10)group and the non-meridian-non-acupoint group,there was significant difference(P<0.05). Conclusion:FGIDs model rats' gastrointestinal microcirculation status on the whole can be improved by EA at Zusanli(ST36),and the possible mechanism is related to the 5-HT content of all horizontal positions,and there is acupoint specificity.
引文
[1]葛均波,徐永健.内科学[M].北京:人民卫生出版社,2014:398.
[2]Coates MD,Tekin I,Vrana KE,et al. The many potential roles of intestinal serotonin(5-hydroxytryptamine,5-HT)signalling in inflammatory bowel disease[J]. Aliment Pharmacol Ther,2017,46(6):569-580.
[3]尹小君,施茵,赵继梦.从脑-肠轴角度探讨肠易激综合征及其中医治疗[J].中华中医药学刊,2011,29(10):2197-2201.
[4]郑春丽,王健,王世军,等.针灸治疗胃肠功能紊乱用穴规律浅析[J].针灸临床杂志,2015,31(9):52-55.
[5]李方,高纺.针刺“足三里”穴干预胃肠功能紊乱-腹泻模型胃肠激素GAS、MTL水平的影响[J].浙江中医药大学学报,2017,41(4):271-273.
[6]柯美云,张艳丽.重视心理因素与功能性胃肠疾病关系的研究[J].诊断学理论与实践,2006,5(1):1-2.
[7]旺建伟,金颖慧,齐德英,等.痛泻要方对脑钠肽含量的作用与脑-肠轴调控相关性的实验研究[J].中医药信息,2011,28(3):15-17.
[8]杨小梅.电针足三里对胃经线上微循环血流灌注量及胃肠功能的影响[D].福州:福建中医药大学,2017.
[9]ZHANG Dong,LI Shunyue,WANG Shuyou,et al. Evaluation of influence of acupuncture and electro-acupuncture for blood perfusion of stomach by Laser Doppler blood perfusion imaging[J].Evidence-Based Complementary and Alternative Medicine,2010,2011(2011):969231
[10]陈磊.电针不同穴位对肠易激综合征模型大鼠肠道微循环、脑肠相关神经肽影响的研究[D].北京:北京中医药大学,2013.
[11]王晓燕,吴富东,王世军,等.电针足三里穴对胃肠功能紊乱模型大鼠脏器微循环的影响及穴位脏腑相关性理论探讨[J].浙江中医药大学学报,2011,35(3):415-417.
[12]王晓燕,吴富东,王世军,等.针刺足三里穴对胃黏膜血流量影响的研究进展[J].针灸临床杂志,2009,25(10):53-54.
[13]娄静,王菲,侯留法.针药并用对腹泻型肠易激综合征患者血清5-羟色胺、降钙素基因相关肽以及内皮素的影响[J].上海针灸杂志,2017,36(8):933.
[14]杨帅,任晓萱,郭孟玮,等.电针“百会”、“足三里”穴对IBS模型大鼠行为及海马5-HT水平的影响[J].现代生物医学进展,2013,10(8):1847.
[15]杜晓娟,汪龙德,刘俊宏,等.功能性消化不良与脑肠轴机制研究进展[J].辽宁中医药大学学报,2017,17(7):116-118.
[16]LachG,SchellekensH,DinanTG,etal.Anxiety,depression,and the microbiome:a role for gut peptides[J].Neurotherapeutics,2018,15(1):36-59.
[17]裴丽霞,朱莉,陈璐,等.针刺对便秘型肠易激综合征患者血浆5-HT、NPY和CGRP的影响[J].南京中医药大学学报,2015,31(5):418.
[18]衣华强,马玉侠,方剑乔.经穴-脏腑相关形态学研究概述[J].山东中医药大学学报,2013,37(1):80-82.
[19]周逸平.经脉-脏腑相关是经络理论的核心[J].针刺研究,1999,24(3):238-240.
[20]童晨光,谷世喆,衣华强,等.胃俞募穴与胃特异性联系通路的荧光双标法研究[J].上海针灸杂志,2003(5):16-19.
[2]Coates MD,Tekin I,Vrana KE,et al. The many potential roles of intestinal serotonin(5-hydroxytryptamine,5-HT)signalling in inflammatory bowel disease[J]. Aliment Pharmacol Ther,2017,46(6):569-580.
[3]尹小君,施茵,赵继梦.从脑-肠轴角度探讨肠易激综合征及其中医治疗[J].中华中医药学刊,2011,29(10):2197-2201.
[4]郑春丽,王健,王世军,等.针灸治疗胃肠功能紊乱用穴规律浅析[J].针灸临床杂志,2015,31(9):52-55.
[5]李方,高纺.针刺“足三里”穴干预胃肠功能紊乱-腹泻模型胃肠激素GAS、MTL水平的影响[J].浙江中医药大学学报,2017,41(4):271-273.
[6]柯美云,张艳丽.重视心理因素与功能性胃肠疾病关系的研究[J].诊断学理论与实践,2006,5(1):1-2.
[7]旺建伟,金颖慧,齐德英,等.痛泻要方对脑钠肽含量的作用与脑-肠轴调控相关性的实验研究[J].中医药信息,2011,28(3):15-17.
[8]杨小梅.电针足三里对胃经线上微循环血流灌注量及胃肠功能的影响[D].福州:福建中医药大学,2017.
[9]ZHANG Dong,LI Shunyue,WANG Shuyou,et al. Evaluation of influence of acupuncture and electro-acupuncture for blood perfusion of stomach by Laser Doppler blood perfusion imaging[J].Evidence-Based Complementary and Alternative Medicine,2010,2011(2011):969231
[10]陈磊.电针不同穴位对肠易激综合征模型大鼠肠道微循环、脑肠相关神经肽影响的研究[D].北京:北京中医药大学,2013.
[11]王晓燕,吴富东,王世军,等.电针足三里穴对胃肠功能紊乱模型大鼠脏器微循环的影响及穴位脏腑相关性理论探讨[J].浙江中医药大学学报,2011,35(3):415-417.
[12]王晓燕,吴富东,王世军,等.针刺足三里穴对胃黏膜血流量影响的研究进展[J].针灸临床杂志,2009,25(10):53-54.
[13]娄静,王菲,侯留法.针药并用对腹泻型肠易激综合征患者血清5-羟色胺、降钙素基因相关肽以及内皮素的影响[J].上海针灸杂志,2017,36(8):933.
[14]杨帅,任晓萱,郭孟玮,等.电针“百会”、“足三里”穴对IBS模型大鼠行为及海马5-HT水平的影响[J].现代生物医学进展,2013,10(8):1847.
[15]杜晓娟,汪龙德,刘俊宏,等.功能性消化不良与脑肠轴机制研究进展[J].辽宁中医药大学学报,2017,17(7):116-118.
[16]LachG,SchellekensH,DinanTG,etal.Anxiety,depression,and the microbiome:a role for gut peptides[J].Neurotherapeutics,2018,15(1):36-59.
[17]裴丽霞,朱莉,陈璐,等.针刺对便秘型肠易激综合征患者血浆5-HT、NPY和CGRP的影响[J].南京中医药大学学报,2015,31(5):418.
[18]衣华强,马玉侠,方剑乔.经穴-脏腑相关形态学研究概述[J].山东中医药大学学报,2013,37(1):80-82.
[19]周逸平.经脉-脏腑相关是经络理论的核心[J].针刺研究,1999,24(3):238-240.
[20]童晨光,谷世喆,衣华强,等.胃俞募穴与胃特异性联系通路的荧光双标法研究[J].上海针灸杂志,2003(5):16-19.