天麻素对脊髓缺血再灌注损伤中脊髓微循环血流量的影响
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摘要
目的:观察腹主动脉内灌注天麻素对兔脊髓缺血再灌注损伤(SCIRI)中脊髓微循环血流量(SCMBF)的影响。方法:60只健康新西兰大白兔随机分为正常组、模型组和天麻素低、中、高剂量组(25,50,100 mg·kg-1),除正常组外,采用肾下腹主动脉阻断法,建立SCIRI模型,分别于缺血前,再灌注15,30,60,120 min监测SCMBF变化。分别于缺血前、再灌注6,12,24 h神经功能评分(NFS)。分别于缺血前、缺血45 min,再灌注60,120 min监测血清神经特异性烯醇化酶浓度(NSE),脊髓组织丙二醛(MDA),活性氧(ROS),谷胱甘肽过氧化物酶(GSH-Px),超氧化物歧化酶(SOD),总抗氧化能力(T-AOC)及脊髓病理学变化。结果:与正常组比较,模型组缺血后NSE浓度,MDA和ROS明显增加(P<0.01),GSH-Px,SOD及T-AOC明显降低(P<0.01)。与模型组比较,缺血后天麻素组NSE,MDA和ROS明显降低(P<0.01),GSH-Px,SOD及T-AOC明显升高(P<0.01)。天麻素组SCMBF和脊髓灰质的病理损害程度明显轻于模型组(P<0.01),NFS较模型组恢复迅速(P<0.01)。结论:SCIRI中腹主动脉内灌注天麻素可通过提高脊髓抗氧化能力来减轻脊髓微循环障碍。
Objective: To observe the effect of gastrodin infused through abdominal aorta on spinal cord microcirculatory blood flow( SCMBF) during the spinal cord ischemia-reperfusion injury( SCIRI). Method:Totally 60 healthy New Zealand rabbits were randomly divided into the normal control group,the model group,gastrodin low,medium and high dose groups( 25,50,100 mg·kg- 1). Except for the normal group,the SCIRI model was created by clamping infrarenal aorta. The changes in SCMBF were monitored before ischemia and at 15,30,60,120 min after the reperfusion. The neurologic function score( NFS) was evaluated before ischemia and at6,12,24 h after the reperfusion. The level of serum neuron-specific enolase( NSE),malondialdehyde( MDA),reactive oxygen species( ROS),glutathione peroxidase( GSH-Px),superoxide dismutase( SOD),total antioxidation capacity( T-AOC) and the pathological changes in spinal cord tissues were monitored before the ischemia and at 45,60,120 min after the reperfusion. Result: Compared with the model group,the model group showed significantly higher NSE,MDA and ROS( P < 0. 01) and lower GSH-Px,SOD and T-AOC( P < 0. 01) after ischemia. NSE,MDA and ROS were significantly lower while GSH-Px,SOD and T-AOC were distinctly higher in the gastrodin group after ischemia than that in the model group( P < 0. 01). The SCMBF and pathological damage in spinal cord in the model group were obviously more serious than that in the gastrodin group( P < 0. 01),but NFS recovered rapidly in the gastrodin group during reperfusion than that in the model group( P < 0. 01).Conclusion: The intra-aortic gastrodin infusion could distinctly alleviate spinal cord microcirculatory dysfunction by infusing gastrodin through abdominal aorta and improving the anti-oxidation ability of spinal cord during SCIRI.
Objective: To observe the effect of gastrodin infused through abdominal aorta on spinal cord microcirculatory blood flow( SCMBF) during the spinal cord ischemia-reperfusion injury( SCIRI). Method:Totally 60 healthy New Zealand rabbits were randomly divided into the normal control group,the model group,gastrodin low,medium and high dose groups( 25,50,100 mg·kg- 1). Except for the normal group,the SCIRI model was created by clamping infrarenal aorta. The changes in SCMBF were monitored before ischemia and at 15,30,60,120 min after the reperfusion. The neurologic function score( NFS) was evaluated before ischemia and at6,12,24 h after the reperfusion. The level of serum neuron-specific enolase( NSE),malondialdehyde( MDA),reactive oxygen species( ROS),glutathione peroxidase( GSH-Px),superoxide dismutase( SOD),total antioxidation capacity( T-AOC) and the pathological changes in spinal cord tissues were monitored before the ischemia and at 45,60,120 min after the reperfusion. Result: Compared with the model group,the model group showed significantly higher NSE,MDA and ROS( P < 0. 01) and lower GSH-Px,SOD and T-AOC( P < 0. 01) after ischemia. NSE,MDA and ROS were significantly lower while GSH-Px,SOD and T-AOC were distinctly higher in the gastrodin group after ischemia than that in the model group( P < 0. 01). The SCMBF and pathological damage in spinal cord in the model group were obviously more serious than that in the gastrodin group( P < 0. 01),but NFS recovered rapidly in the gastrodin group during reperfusion than that in the model group( P < 0. 01).Conclusion: The intra-aortic gastrodin infusion could distinctly alleviate spinal cord microcirculatory dysfunction by infusing gastrodin through abdominal aorta and improving the anti-oxidation ability of spinal cord during SCIRI.
引文
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[3]李海龙,粟艳,厚荣荣,等.天麻素对百草枯和代森锰诱导的小鼠脑黑质多巴胺能神经元损伤的保护作用[J].成都中医药大学学报,2010,33(1):57-59.
[4]Simon F,Scheuerle A,Calzia E,et al.Erythropoietin during porcine aortic balloon occlusion-induced ischemia/reperfusion injury[J].Crit Care Med,2008,36(7):2143-2150.
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[6]Goyagi T,Tobe Y.Dexmedetomidine improves the histological and neurological outcomes 48 h after transient spinal ischemia in rats[J].Brain Res,2014,3(6):24-30.
[7]Badem S,Ugurlucan M,Eg H,et al.Effects of Ginkgo biloba extract on spinal cord ischemia-reperfusion injury in rats[J].Ann Vasc Surg,2014,28(5):1296-1305.
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[9]Kubota K,Saiwai H,Kumamaru H,et al.Neurological recovery is impaired by concurrent but not by asymptomatic pre-existing spinal cord compression after traumatic spinal cord injury[J].Spine,2012,37(17):1448-1455.
[10]Chou A K,Yang M C,Tsai H P,et al.Adenoviralmediated glial cell line-derived neurotrophic factor gene transfer has a protective effect on sciatic nerve following constriction-induced spinal cord injury[J].PLo S One,2014,9(3):264-269.
[11]Ilhan G,Aksun M,Ozpak B,et al.The effect of combined hyperbaric oxygen and iloprost treatment on the prevention of spinal cord ischaemia-reperfusion injury:an experimental study[J].Eur J Cardiothorac Surg,2013,44(5):332-340.
[12]Cui Y,Zhang H,Ji M,et al.Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats[J].J Surg Res,2014,4(14):529-532.
[13]Koriyama Y,Nakayama Y,Matsugo S,et al.Antiinflammatory effects of lipoic acid through inhibition of GSK-3βin lipopolysaccharide-induced BV-2 microglial cells[J].Neurosci Res,2013,77(2):87-96.
[14]Tavuku H H,Sener T E,Tinay I,et al.Melatonin and tadalafil treatment improves erectile dysfunction after spinal cord injury in rats[J].Clin Exp Pharmacol Physiol,2014,41(4):309-316.
[15]赵朝华,吴树强,杨杰,等.天麻素预处理对短暂性脑缺血大鼠脑组织S100β表达的影响[J].中国老年学杂志,2014,4(34):2156-2158.
[16]曾祥慧,葛亚坤,严明,等.天麻素对酸中毒诱导的大鼠海马神经元损伤的保护作用[J].中草药,2007,38(12):1867-1869.
[17]高健美,陆国辉,李艳茹,等.天麻素通过SIRT1/PGC-1α信号通路抗叔丁基过氧化氢诱导的HL7702细胞氧化损伤[J].中药药理与临床,2014,30(4):32-35.
[18]赵燕玲,张博爱,贾延劼,等.天麻素对海人酸致大鼠大脑皮质神经元损伤的保护作用[J].中国实用神经疾病杂志,2009,12(5):33-36.
[19]付蕾,毛艳华,高远,等.天麻素对大鼠皮层缺氧神经细胞NR1亚基mRNA表达的影响[J].中国中药杂志,2008,33(9):1049-1052.
[2]Jan Y K,Liao F,Jones M A,et al.Effect of durations of wheelchair tilt-in-space and recline on skin perfusion over the ischial tuberosity in people with spinal cord injury[J].Arch Phys Med Rehabil,2013,94(4):667-672.
[3]李海龙,粟艳,厚荣荣,等.天麻素对百草枯和代森锰诱导的小鼠脑黑质多巴胺能神经元损伤的保护作用[J].成都中医药大学学报,2010,33(1):57-59.
[4]Simon F,Scheuerle A,Calzia E,et al.Erythropoietin during porcine aortic balloon occlusion-induced ischemia/reperfusion injury[J].Crit Care Med,2008,36(7):2143-2150.
[5]Blaser A,Lang J,Henke D,et al.Influence of durotomy on laser-Doppler measurement of spinal cord blood flow in chondrodystrophic dogs with thoracolumbar disk extrusion[J].Vet Surg,2012,41(2):221-227.
[6]Goyagi T,Tobe Y.Dexmedetomidine improves the histological and neurological outcomes 48 h after transient spinal ischemia in rats[J].Brain Res,2014,3(6):24-30.
[7]Badem S,Ugurlucan M,Eg H,et al.Effects of Ginkgo biloba extract on spinal cord ischemia-reperfusion injury in rats[J].Ann Vasc Surg,2014,28(5):1296-1305.
[8]成令忠,钟翠平,蔡文琴.现代组织学[M].上海:上海科学技术文献出版社,2003:38-39.
[9]Kubota K,Saiwai H,Kumamaru H,et al.Neurological recovery is impaired by concurrent but not by asymptomatic pre-existing spinal cord compression after traumatic spinal cord injury[J].Spine,2012,37(17):1448-1455.
[10]Chou A K,Yang M C,Tsai H P,et al.Adenoviralmediated glial cell line-derived neurotrophic factor gene transfer has a protective effect on sciatic nerve following constriction-induced spinal cord injury[J].PLo S One,2014,9(3):264-269.
[11]Ilhan G,Aksun M,Ozpak B,et al.The effect of combined hyperbaric oxygen and iloprost treatment on the prevention of spinal cord ischaemia-reperfusion injury:an experimental study[J].Eur J Cardiothorac Surg,2013,44(5):332-340.
[12]Cui Y,Zhang H,Ji M,et al.Hydrogen-rich saline attenuates neuronal ischemia-reperfusion injury by protecting mitochondrial function in rats[J].J Surg Res,2014,4(14):529-532.
[13]Koriyama Y,Nakayama Y,Matsugo S,et al.Antiinflammatory effects of lipoic acid through inhibition of GSK-3βin lipopolysaccharide-induced BV-2 microglial cells[J].Neurosci Res,2013,77(2):87-96.
[14]Tavuku H H,Sener T E,Tinay I,et al.Melatonin and tadalafil treatment improves erectile dysfunction after spinal cord injury in rats[J].Clin Exp Pharmacol Physiol,2014,41(4):309-316.
[15]赵朝华,吴树强,杨杰,等.天麻素预处理对短暂性脑缺血大鼠脑组织S100β表达的影响[J].中国老年学杂志,2014,4(34):2156-2158.
[16]曾祥慧,葛亚坤,严明,等.天麻素对酸中毒诱导的大鼠海马神经元损伤的保护作用[J].中草药,2007,38(12):1867-1869.
[17]高健美,陆国辉,李艳茹,等.天麻素通过SIRT1/PGC-1α信号通路抗叔丁基过氧化氢诱导的HL7702细胞氧化损伤[J].中药药理与临床,2014,30(4):32-35.
[18]赵燕玲,张博爱,贾延劼,等.天麻素对海人酸致大鼠大脑皮质神经元损伤的保护作用[J].中国实用神经疾病杂志,2009,12(5):33-36.
[19]付蕾,毛艳华,高远,等.天麻素对大鼠皮层缺氧神经细胞NR1亚基mRNA表达的影响[J].中国中药杂志,2008,33(9):1049-1052.