瘤胃微生物体外降解稻秸和紫花苜蓿干草粉试验
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摘要
利用瘤胃微生物在玻璃三角瓶中严格控制其生存环境条件(温度39~42℃、pH值6.0左右、厌氧、一定的渗透压)下对不同比例的稻草秸秆和紫花苜蓿干草进行体外发酵降解试验,筛选出稻草秸秆和紫花苜蓿干草的最佳比例组合。稻草秸秆和紫花苜蓿干草设计7种不同比例进行两次重复试验,每2 h对产气量进行一次测定,36 h后停止试验,45 h后对试验前后的干物质降解率进行分析,产气量越多、干物质降解率越大,说明瘤胃微生物对该组的发酵降解效果越好。试验结果表明,瘤胃微生物生长繁殖的稻秸和紫花苜蓿干草最佳比例组合为3 7,4 6次之。试验所产生的微生物菌体蛋白质为非草食动物提供动物蛋白质来源,试验所获得的数据可为草食动物的饲料营养价值以及饲养效果研究提供科学参考数据。
Strictly controlled rumen microorganisms living environment conditions(39~42 ℃,pH 6.0 or so, anaerobic, osmotic pressure) in a glass flask, degraded different ratio rice straw and alfalfa hay powder in vitro, filtered out the best ratio combinations of rice straw and alfalfa hay.Designed seven different proportions of rice straw and alfalfa hay, repeated trials twice, mea sured gas production every 2 h, stopped test after 36 h, analyzed dry substance degradation rate before and after the test after 45 h. The more gas production, the dry substance degradation rate is bigger, the fermentation effect is better in this group.The test results show that the best ratio of rumen microbial growth and reproduction of rice straw and alfalfa hay is 3 7, 4 6 fol lowed. Bacterium protein produced by test is protein sourcefor non-herbivores.This test data can provide the scientific reference data of feed nutritional value and feeding effects study for
Strictly controlled rumen microorganisms living environment conditions(39~42 ℃,pH 6.0 or so, anaerobic, osmotic pressure) in a glass flask, degraded different ratio rice straw and alfalfa hay powder in vitro, filtered out the best ratio combinations of rice straw and alfalfa hay.Designed seven different proportions of rice straw and alfalfa hay, repeated trials twice, mea sured gas production every 2 h, stopped test after 36 h, analyzed dry substance degradation rate before and after the test after 45 h. The more gas production, the dry substance degradation rate is bigger, the fermentation effect is better in this group.The test results show that the best ratio of rumen microbial growth and reproduction of rice straw and alfalfa hay is 3 7, 4 6 fol lowed. Bacterium protein produced by test is protein sourcefor non-herbivores.This test data can provide the scientific reference data of feed nutritional value and feeding effects study for
引文
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[2]付钓钧.瘤胃微生物体外降解大豆荚和玉米秸秆混合粉试验[J].饲料工业,2008,29(11):49-50.
[3]付钓钧,王长平,程广东,等.瘤胃微生物体外分解不同比例大豆荚玉米秸秆混合粉产气量的试验[J].饲料与畜牧.2008(1):12-13.
[4]阳伏林,丁学智.秸秆和苜蓿干草不同比例组合对人工瘤胃pH值、氨态氮及产气量的影响[J].饲料工业,2007,28(17):12-13.
[5]付钓钧,赵瑞艳,王长平,等.瘤胃微生物体外发酵不同配比大豆荚与稻草混合粉产气量研究[J].饲料工业,2011,32(3):30-32.
[6]周传社,汤少勋,姜海林,等.农作物秸秆体外发酵营养特性及组合利用研究[J].应用生态学报,2005,16(10):1862-1687.