桃红颈天牛肠道纤维素降解菌的筛选及其对肉仔鸡生产性能的影响
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摘要
文章旨在从桃红颈天牛Aromia bungii Faldermann幼虫肠道中筛选的一株高纤维素酶活菌株,经16S rDNA测序,确定为枯草芽孢杆菌,命名为BSAB1306,进行饲料制粒,探讨其饲料制粒的耐受性和其对肉鸡肠道中微生物区系、生产性能的影响。选择体重相近的20只30日龄白羽肉鸡,先预饲无BSAB1306菌株饲料72 h,空腹2 h,然后饲喂其菌株饲料,1.5 h后屠宰。结果表明:其羧甲基纤维素酶活为68.27 U/ml,对其进行发酵培养后,酶活提高到163.86 U/ml;其饲料制粒后在50℃下保存50 d存活率为94.58%;菌株在十二指肠的萌发率为94.6%;且肉鸡的肠道食糜中大肠杆菌数量明显下降(P<0.05),第二和三周龄的微生物菌体蛋白含量显著增多(P<0.05)。结果提示BSAB1306菌株具有极高的饲料潜能,且可改善肉仔鸡的肠道微生物区系。
In this paper, we screened a strain of higher cellulase activity from Aromia bungii Falder-mann larvae intestinal hydrolysis, It was identified as bacillus subtilis pass through 16 S rDNA sequenc-ing, it was named BSAB1306. It was discussed the influence of intestinal microflora in different weeksbroiler and productivity on feed pelleting of BSAB1306 strain. We selected 20 similar weight white feath-er broiler in 30 day-old. We preliminary fed without BSAB1306 strain fodder of 72 h, empty stom-ach 2 h, and then we fed BSAB1306 strain fodder before to slaughter 1.5 h later. The results showedthat its CMC enzyme activity was 68.27 U/ml, and its CMC enzyme activity was improved to 163.86 U/ml after fermentation. We found that the strain survival rate still attained to 94.58% on preserving in50 ℃ and its germination rate was 94.58% in the duodenum after feeding pelleting. Moreover, wefound the number of bacillus coli dramatically declined in the intestinal tract chyme, and the contentof bacterial protein significantly increased in intestinal chyme of 2 weeks and 3 weeks chicken whenfed broiler chicken with complex BSAB1306 strain. The experiment proves that the BSAB1306 strainhad high potential fodder application and it could improve the intestinal microflora of broiler chickens.
In this paper, we screened a strain of higher cellulase activity from Aromia bungii Falder-mann larvae intestinal hydrolysis, It was identified as bacillus subtilis pass through 16 S rDNA sequenc-ing, it was named BSAB1306. It was discussed the influence of intestinal microflora in different weeksbroiler and productivity on feed pelleting of BSAB1306 strain. We selected 20 similar weight white feath-er broiler in 30 day-old. We preliminary fed without BSAB1306 strain fodder of 72 h, empty stom-ach 2 h, and then we fed BSAB1306 strain fodder before to slaughter 1.5 h later. The results showedthat its CMC enzyme activity was 68.27 U/ml, and its CMC enzyme activity was improved to 163.86 U/ml after fermentation. We found that the strain survival rate still attained to 94.58% on preserving in50 ℃ and its germination rate was 94.58% in the duodenum after feeding pelleting. Moreover, wefound the number of bacillus coli dramatically declined in the intestinal tract chyme, and the contentof bacterial protein significantly increased in intestinal chyme of 2 weeks and 3 weeks chicken whenfed broiler chicken with complex BSAB1306 strain. The experiment proves that the BSAB1306 strainhad high potential fodder application and it could improve the intestinal microflora of broiler chickens.
引文
[1]Sun J Z And Scharf M E.Insects and Biofuels[M].In English:Insect Science,2010,V17:163-333.
[2]Sugimura M,Watanabe H,Lo N,et al.Purification,characterization,c DNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle,Psacothea hilaris[J].European Journal of Biochemistry,2003,270(16):3455-3460.
[3]Wei Y D,Lee K S,Gui Z Z,et al.Molecular cloning,expression,and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle,Apriona germari[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2006,145(2):220-229.
[4]刘晨娟,蔡皓,李庆,等.桑粒肩天牛肠道木质纤维素分解细菌的分离和鉴定[J].化工与生物工程,2010,27(7):66-68.
[5]Samanya M,Yamauchi K E.Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var.natto[J].Comparative Biochemistry and Physiology Part A:Molecular and Integrative Physiology,2002,133(1):95-104.
[6]La R R,Casula G,Cutting S M,et al.Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry[J].Veterinary Microbiology,2001,79(2):133-142.
[7]Haghighi H R,Gong J,Gyles C L,et al.Probiotics stimulate production of natural antibodies in chickens[J].Clinical and Vaccine Immunology:CVI,2006,13(9):975-980.
[8]郝牛宏,董晓芳,佟建明,等.不同饲料制粒条件和保存时间对益生菌活力的影响[J].中国畜牧杂志,2009,45(1):51-53.
[9]中华人民共和国国家技术监督局.GB/T 14699.1-2005[S].中华人民共和国国家标准-饲料与采样.中国标准出版社,2005-03-23.
[10]Lee S J,Lee K S,Kim S R,et al.A novel cellulase gene from the mulberry longicorn beetle,Apriona germari:gene structure,expression,and enzymatic activity[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2005,140:551-560.
[11]Geib S M,Jimenez Gasco M M,Carlson J E,et al.Effect of host tree species on cellulase activity and bacterial community composition in the gut of larval Asian longhorned beetle[J].Environmental Entomology,2009,38(3):686-699.
[12]Eutick M L,Brien B W,Slaytor M.Bacteria from the gut of Australian termites[J].Appl Environ Microbiot,1978,35(5):823-828.
[13]高云航,张喜宏,刘佳丽,等.鹅肠道纤维素分解菌的分离鉴定及其产酶条件的优化[J].动物营养学报,2011,23(3):466-472.
[14]Vila B,Fontgibell A,Badiola I,et al.Reduction of Salmonella enterica var.Enteritidis colonization and invasion by Bacillus cereus var.toyoi inclusion in poultry feeds[J].Poultry Science,2009,88(5):975-979.
[15]Midilli M,Alp M,Kocabagh N,et al.Effects of dietary probiotic and prebiotic supplementation on growth performance and serum Ig G concentration of broilers[J].South African Journal of Animal Science,2008,37:21-27.
[16]Edens F W.An alternative for antibiotic use in poultry:Probiotics[J].Brazilian Journal of Poultry Science,2003,5(2):75-97.
[2]Sugimura M,Watanabe H,Lo N,et al.Purification,characterization,c DNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle,Psacothea hilaris[J].European Journal of Biochemistry,2003,270(16):3455-3460.
[3]Wei Y D,Lee K S,Gui Z Z,et al.Molecular cloning,expression,and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle,Apriona germari[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2006,145(2):220-229.
[4]刘晨娟,蔡皓,李庆,等.桑粒肩天牛肠道木质纤维素分解细菌的分离和鉴定[J].化工与生物工程,2010,27(7):66-68.
[5]Samanya M,Yamauchi K E.Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var.natto[J].Comparative Biochemistry and Physiology Part A:Molecular and Integrative Physiology,2002,133(1):95-104.
[6]La R R,Casula G,Cutting S M,et al.Bacillus subtilis spores competitively exclude Escherichia coli O78:K80 in poultry[J].Veterinary Microbiology,2001,79(2):133-142.
[7]Haghighi H R,Gong J,Gyles C L,et al.Probiotics stimulate production of natural antibodies in chickens[J].Clinical and Vaccine Immunology:CVI,2006,13(9):975-980.
[8]郝牛宏,董晓芳,佟建明,等.不同饲料制粒条件和保存时间对益生菌活力的影响[J].中国畜牧杂志,2009,45(1):51-53.
[9]中华人民共和国国家技术监督局.GB/T 14699.1-2005[S].中华人民共和国国家标准-饲料与采样.中国标准出版社,2005-03-23.
[10]Lee S J,Lee K S,Kim S R,et al.A novel cellulase gene from the mulberry longicorn beetle,Apriona germari:gene structure,expression,and enzymatic activity[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2005,140:551-560.
[11]Geib S M,Jimenez Gasco M M,Carlson J E,et al.Effect of host tree species on cellulase activity and bacterial community composition in the gut of larval Asian longhorned beetle[J].Environmental Entomology,2009,38(3):686-699.
[12]Eutick M L,Brien B W,Slaytor M.Bacteria from the gut of Australian termites[J].Appl Environ Microbiot,1978,35(5):823-828.
[13]高云航,张喜宏,刘佳丽,等.鹅肠道纤维素分解菌的分离鉴定及其产酶条件的优化[J].动物营养学报,2011,23(3):466-472.
[14]Vila B,Fontgibell A,Badiola I,et al.Reduction of Salmonella enterica var.Enteritidis colonization and invasion by Bacillus cereus var.toyoi inclusion in poultry feeds[J].Poultry Science,2009,88(5):975-979.
[15]Midilli M,Alp M,Kocabagh N,et al.Effects of dietary probiotic and prebiotic supplementation on growth performance and serum Ig G concentration of broilers[J].South African Journal of Animal Science,2008,37:21-27.
[16]Edens F W.An alternative for antibiotic use in poultry:Probiotics[J].Brazilian Journal of Poultry Science,2003,5(2):75-97.