微量热泳动技术的生物医学研究进展
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摘要
微量热泳动技术基于荧光标记分子在温度梯度内的定向移动,能够在溶液中快速、灵敏地测量生物相关分子间的相互作用。它不需要表面固定和大量样品,对相对分子质量也没有限制。热泳动对分子的大小、电荷和水化层的微小变化都十分敏感,适合于蛋白质、核酸、小分子和离子等多种物质的相互作用研究。微量热泳动技术由于其独特的优势,目前已经在生物医学领域中有了初步应用。本文介绍了微量热泳动技术的背景和其在生物医学领域中不同生物相关分子间相互作用分析的研究进展。
Microscale thermophoresis(MST) technology is based on the directed movements of fluorescently labeled molecules in a temperature gradient, and it can quickly and sensitively quantify biologically related molecule interactions in free solutions. It doesn't need surface immobilization and high sample consumption, and has no limitation with molecular weight. Thermophoresis is sensitive to the tiny changes of molecular size, charge and hydration shell, and is suitable for the biologically related molecule interactions research, such as protein, nucleic acids, small molecules, ions and other chemicals. Because of its unique advantages, MST has primarily applied in biomedical field. In this review, we present the background, progress and developments of MST in the biologically related molecule interactions in biomedical fields.
Microscale thermophoresis(MST) technology is based on the directed movements of fluorescently labeled molecules in a temperature gradient, and it can quickly and sensitively quantify biologically related molecule interactions in free solutions. It doesn't need surface immobilization and high sample consumption, and has no limitation with molecular weight. Thermophoresis is sensitive to the tiny changes of molecular size, charge and hydration shell, and is suitable for the biologically related molecule interactions research, such as protein, nucleic acids, small molecules, ions and other chemicals. Because of its unique advantages, MST has primarily applied in biomedical field. In this review, we present the background, progress and developments of MST in the biologically related molecule interactions in biomedical fields.
引文
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[20]Liu Y,Liu N,Ma XH,et al.Highly specific detection of thrombin using an aptamer-based suspension array and the interaction analysis via microscale thermophoresis.Analyst,2015,140:2762-2770
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[22]Ascher DB,Wielens J,Nero TL,et al.Potent hepatitis C inhibitors bind directly to NS5A and reduce its affinity for RNA.Sci Rep,2014,4:4765
[23]Mecozzi VJ,Berman DE,Simoes S,et al.Pharmacological chaperones stabilize retromer to limit APP processing.Nat Chem Biol,2014,10:443-449
[24]Bogaart G,Meyenberg K,Diederichsen U,et al.Phosphatidylinositol 4,5-Bisphosphate increases Ca2+affinity of synaptotagmin-1 by 40-fold.J Biol Chem,2012,287:16447-16453
[25]Entzian C,Schubert T.Studying small molecule-aptamer interactions using Micro Scale Thermophoresis(MST).Methods,2015,97:27-34
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[27]Wolff M,Braun D,Nash MA.Detection of thermoresponsive polymer phase transition in dilute low-volume format by microscale thermophoretic depletion.Anal Chem,2014,86:6797-6803
[28]Wienken CJ,Baaske P,Duhr S,et al.Thermophoretic melting curves quantify the conformation and stability of RNA and DNA.Nucleic Acids Res,2011,39:e52
[29]艾秋实,曹向宇,赵芊,等.微量热泳动技术原理及其在研究生物分子互作方面的应用.生物技术通报,2015,31:67-73
[30]Antosiewicz A,Senkara E,Cie?la J.Quartz crystal microbalance with dissipation and microscale thermophoresis as tools for investigation of protein complex formation between thymidylate synthesis cycle enzymes.Biosens Bioelectron,2015,64:36-42
[31]Martin JA,Chávez JL Chushak Y,et al.Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol.Anal Bioanal Chem,2014,406:4637-4647
[2]Wienken CJ,Duhr S,Baaske P.Nano Temper:some like it hot.New Biotechnol,2010,27:106-107
[3]Jerabek-Willemsen M,Wienken C J,Braun D,et al.Molecular interaction studies using microscale thermophoresis.Assay Drug Dev Techn,2011,9:342-353
[4]Jerabek-Willemsena M,AndréT,Wanner R,et al.Micro Scale thermophoresis:interaction analysis and beyond.J Mol Struct,2014,1077:101-113
[5]Mao Y,Yu L,Yang R,et al.A novel method for the study of molecular interaction by using microscale thermophoresis.Talanta,2015,132:894-901
[6]Baaske P,Wienken CJ,Reineck P,et al.Optical thermophoresis for quantifying the buffer dependence of aptamer binding.Angew Chem Int Ed,2010,49:1-5
[7]Seidel SAI,Dijkman PM,Lea WA,et al.Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions.Methods,2013,59:301-315
[8]Wienken CJ,Baaske P,Rothbauer U,et al.Protein-binding assays in biological liquids using microscale thermophoresis.Nat Commun,2010,1:100
[9]Seidel SAI,Wienken CJ,Geissler S,et al.Label-free microscale thermophoresis discriminates sites and affinity of protein–ligand binding.Angew Chem Int Ed,2012,51:10656-10659
[10]Breitsprecher D,Schlinck N,Witte D,et al.Aptamer binding studies using microscale thermophoresis.Methods Mol Biol,2016,1380:99-111
[11]Wolff M,Mittag J,Herling T,et al.Quantitative thermophoretic study of disease-related protein aggregates.Sci Rep,2016,6:22829
[12]Allam R,Scherbaum CR,Darisipudi MN,et al.Histones from dying renal cells aggravate kidney injury via TLR2and TLR4.J Am Soc Nephrol,2012,23:1375-1388
[13]Langelueddecke C,Roussa E,Fenton RA,et al.Lipocalin-2(24p3/neutrophil gelatinase-associated lipocalin(NGAL)receptor is expressed in distal nephron and mediates protein endocytosis.J Biol Chem,2012,287:159-169
[14]曹木青,潘俊敏.纤毛及纤毛相关疾病研究进展.中国细胞生物学学报,2012,34:849-856
[15]Bhogaraju S,Cajanek L,Fort C,et al.Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.Science,2013,341:1009-1012
[16]Zhao Y,Niu C,Wen X,et al.The minimum activation peptide from ilv H can activate the catalytic subunit of AHAS from different species.Chem Bio Chem,2013,14,746-752
[17]Miles La,Crespi GAN,Doughty L,et al.Bapineuzumab captures the N-terminus of the Alzheimer’s disease amyloid-beta peptide in a helical conformation.Sci Rep,2013,1302:1-5
[18]Lippok S,Seidel SAI,Duhr S,et al.Direct detection of antibody concentration and affinity in human serum using microscale thermophoresis.Anal Chem,2012,84:3523-3530
[19]Zillner K,Jerabek-Willemsen M,Duhr S,et al.Microscale thermophoresis as a sensitive method to quantify protein:nucleic acid interactions in solution.Methods Mol Biol,2012,815:241-252
[20]Liu Y,Liu N,Ma XH,et al.Highly specific detection of thrombin using an aptamer-based suspension array and the interaction analysis via microscale thermophoresis.Analyst,2015,140:2762-2770
[21]Bock LC,Griffin LC,Latham JA,et al.Selection of single-stranded DNA molecules that bind and inhibit human thrombin.Nature,1992,355:564-566
[22]Ascher DB,Wielens J,Nero TL,et al.Potent hepatitis C inhibitors bind directly to NS5A and reduce its affinity for RNA.Sci Rep,2014,4:4765
[23]Mecozzi VJ,Berman DE,Simoes S,et al.Pharmacological chaperones stabilize retromer to limit APP processing.Nat Chem Biol,2014,10:443-449
[24]Bogaart G,Meyenberg K,Diederichsen U,et al.Phosphatidylinositol 4,5-Bisphosphate increases Ca2+affinity of synaptotagmin-1 by 40-fold.J Biol Chem,2012,287:16447-16453
[25]Entzian C,Schubert T.Studying small molecule-aptamer interactions using Micro Scale Thermophoresis(MST).Methods,2015,97:27-34
[26]Kotyrba UM,Propper K,Sachs EF,et al.Triostin A derived cyclopeptide as architectural template for the alignment of four recognition units.Chem Open,2014,3:152-160
[27]Wolff M,Braun D,Nash MA.Detection of thermoresponsive polymer phase transition in dilute low-volume format by microscale thermophoretic depletion.Anal Chem,2014,86:6797-6803
[28]Wienken CJ,Baaske P,Duhr S,et al.Thermophoretic melting curves quantify the conformation and stability of RNA and DNA.Nucleic Acids Res,2011,39:e52
[29]艾秋实,曹向宇,赵芊,等.微量热泳动技术原理及其在研究生物分子互作方面的应用.生物技术通报,2015,31:67-73
[30]Antosiewicz A,Senkara E,Cie?la J.Quartz crystal microbalance with dissipation and microscale thermophoresis as tools for investigation of protein complex formation between thymidylate synthesis cycle enzymes.Biosens Bioelectron,2015,64:36-42
[31]Martin JA,Chávez JL Chushak Y,et al.Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol.Anal Bioanal Chem,2014,406:4637-4647