MST技术在生命科学中的应用进展
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摘要
在生命科学领域中,生物分子间的相互作用具有非常重要的作用。通过分子间相互作用分析不仅可阐明细胞生物学事件,而且为疾病发生机制和药物发现提供基础。MST技术是一种基于检测在温度梯度中的生物分子电泳迁移率的变化而检测生物分子间结合、解离过程,获取分子间相互作用的模式和动力学常数等方面信息的新技术,是近年来发展的研究生物分子相互作用的强有力工具,已广泛应用于生命科学领域研究。本文综述了MST的技术原理、分析方法及其在生命科学领域的应用进展。
Binding events of biomolecules play a central role in all fields of life science.Biomolecular interaction analysis does not only give fundamental insights into the molecular biology of the cell but also paves the way of disease occurrence mechanism and discovery of the new drug.The microscale themophoresis(MST) is a new technology based on the change of thermophoresis motion rate of molecules in temperature gradients,which can detect biomolecules real-time association and dissociation,the information of interactive model,dynamics constant and so on.MST is a power tool of biomolecular interaction analysis and it has been widely applied in life science.This article reviews the principle,analytic method and the further development of MST technology in life science.
Binding events of biomolecules play a central role in all fields of life science.Biomolecular interaction analysis does not only give fundamental insights into the molecular biology of the cell but also paves the way of disease occurrence mechanism and discovery of the new drug.The microscale themophoresis(MST) is a new technology based on the change of thermophoresis motion rate of molecules in temperature gradients,which can detect biomolecules real-time association and dissociation,the information of interactive model,dynamics constant and so on.MST is a power tool of biomolecular interaction analysis and it has been widely applied in life science.This article reviews the principle,analytic method and the further development of MST technology in life science.
引文
[1]Jerabek-Willemsen M,AndréT,Wanner R,et al.Microscale thermophoresis:interaction analysis and beyond[J].J Mol Struct,2014,1077:101-113
[2]Mao YX,Yu LL,Yang R.A novel method for the study of molecular interaction by using microscale thermophoresis[J].Talanta,2015,132:894-901
[3]Zillner K.In:Kaufmann M,Klinger C eds.Functional genomics:methods and protocols,methods in molecular biology[M].Berlin:Springer Science+Business Media LLC,2012,815:241-252
[4]Scheuermann TH,Padrick SB,Gardner KH,et al.On the acquisition and analysis of microscale thermophoresis data[J].Anal Biochem,2016,496:79-93
[5]Seidel SAI,Dijkman PM,Lea WA,et al.Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions[J].Methods,2013,59:301-315
[6]Zhang XP,Zhang L,Zhang J,et al.Design,synthesis and preliminary activity evaluation of novel 3-amino-2-hydroxyl-3-phenylpropanoic acid derivatives as aminopeptidase N/CD13 inhibitors[J].J Enzyme Inhib Med Chem,2013,28(3):545-551
[7]Davis MI,Gross S,Shen M,et al.Biochemical,cellular,and biophysical characterization of a potent inhibitor of mutant isocitrate dehydrogenase IDH1[J].J Biol Chem,2014,289(20):13717-13725
[8]Syntia F,NehméR,Claude B,et al.Human neutrophil elastase inhibition studied by capillary electrophoresis with laser induced fluorescence detection and microscale thermophoresis[J].J Chromatogr A,2016,1431:215-223
[9]Winiewska M,Kucińska K,Makowska M,et al.Thermodynamics parameters for binding of halogenated benzotriazole inhibitors of human protein kinase CK2α[J].Biochim Biophys Acta,2015,1854(10 Pt B):1708-1717
[10]Wasik R,Winska P,Poznański J,et al.Isomeric mono-,di-,and tribromobenzo-1H-triazoles as inhibitors of human protein kinase CK2 alpha[J].PLo S One,2012,7(11):e48898
[11]Shirokova E,Raguse JD,Meyerhof W,et al.The human vomeronasal type-1 receptor family-detection of volatiles and c AMP signaling in He La/Olf cells[J].FASEB J,2008,22:1416-1425
[12]Corin K,Baaske P,Geissler S,et al.Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1[J].Sci Rep,2011,1:172;doi:10.1038/srep00172
[13]Corin K,Baaske P,Ravel DB,et al.A robust and rapid method of producing soluble,stable,and functional G-protein coupled receptors[J].PLo S One,2011,6(10):e23036
[14]Corin K,Baaske P,Ravel DB,et al.Designer lipid-like peptides:A class of detergents for studying functional olfactory receptors using commercial cell-free systems[J].PLo S One,2011,6(11):e25067
[15]Wang XQ,Corin K,Baaske P,et al.Peptide surfactants for cell-free production of functional G protein-coupled receptors[J].PNAS,2011,108(22):9049-9054
[16]Langelueddecke C,Roussa E,Fenton RA,et al.Expression and function of the lipocalin-2(24p3/NGAL)receptor in rodent and human intestinal epithelia[J].PLo S one,2013,8(8):e71586
[17]Basters A,Geurink PP,Oualid FE,et al.Molecular characterization of ubiquitin-specific protease 18 reveals substrate specificity for interferon-stimulated gene 15[J].FEBS J,2014,281:1918-1928
[18]Dijkman PM,Watts A.Lipid modulation of early G protein-coupled receptor signalling events[J].Biochim Biophys Acta,2015,1848:2889-2897
[19]Fedosyuk S,Grishkovskaya I,de Almeida Ribeiro E Jr,et al.Characterization and structure of the vaccinia virus NF-κB antagonist A46[J].J Biol Chem,2014,289(6):3749-3762
[20]Olling A,Hüls C,Goy S et al.The combined repetitive oligopeptides of clostridium difficile toxin A counteract premature cleavage of the glucosyl-transferase domain by stabilizing protein conformation[J].Toxins,2014,6:2162-2176
[21]Takacs M,Petoukhov MV,Atkinson RA,et al.The asymmetric binding of PGC-1a to the ERRa and ERRc nuclear receptor homodimers involves a similar recognition mechanism[J].PLo S one,2013,8(7):e67810
[22]譒tambuk N,Manojlovi Z,Tur i P,et al.A simple three-step method for design and affinity testing of new antisense peptides:an example of erythropoietin[J].Int J Mol Sci,2014,15:9209-9223
[23]Liu H,Thaker YR,Stagg L,et al.SLP-76 sterile motif(SAM)and individual H5 helix mediate oligomer formation for microclusters and T-cell activation[J].J Biol Chem,2013,288:29539-29549
[24]Varizhuk AM,Tsvetkov VB,Tatarinova ON,et al.Synthesis,characterization and in vitro activity of thrombin-binding DNA aptamers with triazole internucleotide linkages[J].Eur J Med Chem,2013,67:90-97
[25]Liu YN,Liu N,Ma XH,et al.Highly specific detection of thrombin using an aptamer-based suspension array and the interaction analysis via microscale thermophoresis[J].Analyst,2015,140:2762-2770
[26]Stoltenburg R,Schubert T,Strehlitz B.In vitro selection and interaction studies of a DNA aptamer targeting protein A[J].PLo S one,2015,10(7):e0134403
[27]Silvers R,Saxena K,Kudlinzki D,et al.Recombinant expression and purification of human TATA binding protein using a chimeric fusion[J].Protein Expres Purif,2012,85:142-147
[28]Entzian C,Schubert T.Studying small molecule-aptamer interactions using microscale thermophoresis(MST)[J].Methods,2016,97:27-34
[29]Martin JA,Chávez JL,Chushak Y,et al.Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol[J].Anal Bioanal Chem,2014,406:4637-4647
[30]Yamniuk AP,Newitt JA,Doyle ML et al.Development of a model protein interaction pair as a benchmarking tool for the quantitative analysis of 2-Site protein-protein interactions[J].J Biomol Tech,2015,26:125-141
[31]Puiu M,Bala C.SPR and SPR Imaging:Recent trends in developing nanodevices for detection and real-Time monitoring of biomolecular events[J].Sensors,2016,16:870;doi:10.3390/s16060870
[32]Makowska J,Wyrzykowski D,Hirniak P,et al.Investigations of copper(II)complexation by fragments of the FBP28 protein using isothermal titration(ITC)and differential scanning calorimetry(DSC)[J].J Therm Anal Calorim,2015,121:263-268
[2]Mao YX,Yu LL,Yang R.A novel method for the study of molecular interaction by using microscale thermophoresis[J].Talanta,2015,132:894-901
[3]Zillner K.In:Kaufmann M,Klinger C eds.Functional genomics:methods and protocols,methods in molecular biology[M].Berlin:Springer Science+Business Media LLC,2012,815:241-252
[4]Scheuermann TH,Padrick SB,Gardner KH,et al.On the acquisition and analysis of microscale thermophoresis data[J].Anal Biochem,2016,496:79-93
[5]Seidel SAI,Dijkman PM,Lea WA,et al.Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions[J].Methods,2013,59:301-315
[6]Zhang XP,Zhang L,Zhang J,et al.Design,synthesis and preliminary activity evaluation of novel 3-amino-2-hydroxyl-3-phenylpropanoic acid derivatives as aminopeptidase N/CD13 inhibitors[J].J Enzyme Inhib Med Chem,2013,28(3):545-551
[7]Davis MI,Gross S,Shen M,et al.Biochemical,cellular,and biophysical characterization of a potent inhibitor of mutant isocitrate dehydrogenase IDH1[J].J Biol Chem,2014,289(20):13717-13725
[8]Syntia F,NehméR,Claude B,et al.Human neutrophil elastase inhibition studied by capillary electrophoresis with laser induced fluorescence detection and microscale thermophoresis[J].J Chromatogr A,2016,1431:215-223
[9]Winiewska M,Kucińska K,Makowska M,et al.Thermodynamics parameters for binding of halogenated benzotriazole inhibitors of human protein kinase CK2α[J].Biochim Biophys Acta,2015,1854(10 Pt B):1708-1717
[10]Wasik R,Winska P,Poznański J,et al.Isomeric mono-,di-,and tribromobenzo-1H-triazoles as inhibitors of human protein kinase CK2 alpha[J].PLo S One,2012,7(11):e48898
[11]Shirokova E,Raguse JD,Meyerhof W,et al.The human vomeronasal type-1 receptor family-detection of volatiles and c AMP signaling in He La/Olf cells[J].FASEB J,2008,22:1416-1425
[12]Corin K,Baaske P,Geissler S,et al.Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1[J].Sci Rep,2011,1:172;doi:10.1038/srep00172
[13]Corin K,Baaske P,Ravel DB,et al.A robust and rapid method of producing soluble,stable,and functional G-protein coupled receptors[J].PLo S One,2011,6(10):e23036
[14]Corin K,Baaske P,Ravel DB,et al.Designer lipid-like peptides:A class of detergents for studying functional olfactory receptors using commercial cell-free systems[J].PLo S One,2011,6(11):e25067
[15]Wang XQ,Corin K,Baaske P,et al.Peptide surfactants for cell-free production of functional G protein-coupled receptors[J].PNAS,2011,108(22):9049-9054
[16]Langelueddecke C,Roussa E,Fenton RA,et al.Expression and function of the lipocalin-2(24p3/NGAL)receptor in rodent and human intestinal epithelia[J].PLo S one,2013,8(8):e71586
[17]Basters A,Geurink PP,Oualid FE,et al.Molecular characterization of ubiquitin-specific protease 18 reveals substrate specificity for interferon-stimulated gene 15[J].FEBS J,2014,281:1918-1928
[18]Dijkman PM,Watts A.Lipid modulation of early G protein-coupled receptor signalling events[J].Biochim Biophys Acta,2015,1848:2889-2897
[19]Fedosyuk S,Grishkovskaya I,de Almeida Ribeiro E Jr,et al.Characterization and structure of the vaccinia virus NF-κB antagonist A46[J].J Biol Chem,2014,289(6):3749-3762
[20]Olling A,Hüls C,Goy S et al.The combined repetitive oligopeptides of clostridium difficile toxin A counteract premature cleavage of the glucosyl-transferase domain by stabilizing protein conformation[J].Toxins,2014,6:2162-2176
[21]Takacs M,Petoukhov MV,Atkinson RA,et al.The asymmetric binding of PGC-1a to the ERRa and ERRc nuclear receptor homodimers involves a similar recognition mechanism[J].PLo S one,2013,8(7):e67810
[22]譒tambuk N,Manojlovi Z,Tur i P,et al.A simple three-step method for design and affinity testing of new antisense peptides:an example of erythropoietin[J].Int J Mol Sci,2014,15:9209-9223
[23]Liu H,Thaker YR,Stagg L,et al.SLP-76 sterile motif(SAM)and individual H5 helix mediate oligomer formation for microclusters and T-cell activation[J].J Biol Chem,2013,288:29539-29549
[24]Varizhuk AM,Tsvetkov VB,Tatarinova ON,et al.Synthesis,characterization and in vitro activity of thrombin-binding DNA aptamers with triazole internucleotide linkages[J].Eur J Med Chem,2013,67:90-97
[25]Liu YN,Liu N,Ma XH,et al.Highly specific detection of thrombin using an aptamer-based suspension array and the interaction analysis via microscale thermophoresis[J].Analyst,2015,140:2762-2770
[26]Stoltenburg R,Schubert T,Strehlitz B.In vitro selection and interaction studies of a DNA aptamer targeting protein A[J].PLo S one,2015,10(7):e0134403
[27]Silvers R,Saxena K,Kudlinzki D,et al.Recombinant expression and purification of human TATA binding protein using a chimeric fusion[J].Protein Expres Purif,2012,85:142-147
[28]Entzian C,Schubert T.Studying small molecule-aptamer interactions using microscale thermophoresis(MST)[J].Methods,2016,97:27-34
[29]Martin JA,Chávez JL,Chushak Y,et al.Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol[J].Anal Bioanal Chem,2014,406:4637-4647
[30]Yamniuk AP,Newitt JA,Doyle ML et al.Development of a model protein interaction pair as a benchmarking tool for the quantitative analysis of 2-Site protein-protein interactions[J].J Biomol Tech,2015,26:125-141
[31]Puiu M,Bala C.SPR and SPR Imaging:Recent trends in developing nanodevices for detection and real-Time monitoring of biomolecular events[J].Sensors,2016,16:870;doi:10.3390/s16060870
[32]Makowska J,Wyrzykowski D,Hirniak P,et al.Investigations of copper(II)complexation by fragments of the FBP28 protein using isothermal titration(ITC)and differential scanning calorimetry(DSC)[J].J Therm Anal Calorim,2015,121:263-268