基于微量量热法的不同来源黄芩抑菌活性对比研究
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摘要
目的:构建基于生物热技术的不同来源黄芩抑菌活性区分方法,基于生物热技术考察不同来源黄芩的抑菌活性差异。方法:采用微量量热技术,研究不同来源黄芩对大肠杆菌生长代谢的热动力学参数的影响,考察栽培区域、采收期、生长年限和种质等的差异对黄芩抑菌活性的影响。结果:不同产地黄芩热动力学参数特征差异较大,其抑制率在24.3%~129.5%,其中河北承德和内蒙古武川黄芩抑菌活性显著高于山东莒县和山西运城;随着药材采收月份的变化(7—11月),黄芩的抑菌活性出现了先下降后急剧增加的现象,且在11月份抑菌活性达到最强;对比不同生长年限的黄芩(2~5年),总体上黄芩药材的抑菌活性随着药材生长年限的增加而增强;多点试验考察不同种质黄芩药材抑菌活性发现,不同种质黄芩抑菌活性差异较大,其表现出来的抑菌活性与纬度跨越有关。结论:不同产地黄芩抑菌活性存在一定的差异,药材的采收时间、生长年限以及种质等的差异均能对黄芩抑菌率产生影响。基于生物热技术的微量量热技术用于不同来源药材的区分,较传统的药理、化学和形态学方法具有一定的便捷性和快速性,可作为进一步区分不同来源药材药效差异的依据之一。
Objective: To study the bacteriostatic activity of different Scutellaria baicalensis based on the microcalorimetric technique.Methods: The effects of different S.baicalensis on the thermokinetics parameters of Escherichia coli metabolism were studied by using the technology of microcalorimetric,and the differences of bacteriostatic activity of S.baicalensis on different cultivation area,harvesting time,growing year and germplasm were compered.Results: The results showed the differences about thermokinetics parameters among the differences cultivation area.The bacteriostatic activity changed between24.3% and 129.5%,and the samples root from Hebei and Neimeng showed stronger bacteriostatic activity than those from Shangdong and Shangxi.It exhibited the first declining later increasing of bacteriostatic activity with the harvesting time changing(from the July to the November),and the strongest bacteriostatic activity occurred in November.There were also the trends of gradually increase on the bacteriostatic activity with the increasing years of planting(from two years to five years).The experimental result of multi-site testes showed a big difference among the different plant germplasm,it should be a relevance between bacteriostatic activity and the spanning of latitude across.Conclusion: There exist some differences among these samples from difference cultivated area and the harvesting time,growing years and germplasm should also affect the bacteriostatic activity.It is concluded that the method based on the microcalorimetric technique could be used to distinguish the different S.baicalensis,and is more convenient and faster,also provide more information for distinguish pharmacological activity of different drug sources.
Objective: To study the bacteriostatic activity of different Scutellaria baicalensis based on the microcalorimetric technique.Methods: The effects of different S.baicalensis on the thermokinetics parameters of Escherichia coli metabolism were studied by using the technology of microcalorimetric,and the differences of bacteriostatic activity of S.baicalensis on different cultivation area,harvesting time,growing year and germplasm were compered.Results: The results showed the differences about thermokinetics parameters among the differences cultivation area.The bacteriostatic activity changed between24.3% and 129.5%,and the samples root from Hebei and Neimeng showed stronger bacteriostatic activity than those from Shangdong and Shangxi.It exhibited the first declining later increasing of bacteriostatic activity with the harvesting time changing(from the July to the November),and the strongest bacteriostatic activity occurred in November.There were also the trends of gradually increase on the bacteriostatic activity with the increasing years of planting(from two years to five years).The experimental result of multi-site testes showed a big difference among the different plant germplasm,it should be a relevance between bacteriostatic activity and the spanning of latitude across.Conclusion: There exist some differences among these samples from difference cultivated area and the harvesting time,growing years and germplasm should also affect the bacteriostatic activity.It is concluded that the method based on the microcalorimetric technique could be used to distinguish the different S.baicalensis,and is more convenient and faster,also provide more information for distinguish pharmacological activity of different drug sources.
引文
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[6]张学儒,王伽伯,肖小河,等.大黄药材两种化学型对金葡菌生长代谢热动力学变化的影响[J].药学学报,2010,45(9):1144-1148.
[7]周丹蕾,鄢丹,李宝才,等.微量量热法研究天然虫草和人工虫草对大肠杆菌生长代谢的影响[J].药学学报,2009,44(6):640-644.
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[10]赵艳玲,王伽伯,肖小河.微量热法研究板蓝根的生物热力学特征[J].中草药,2007,38(2):193-196.
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[12]宋旦哥,孟庆刚.黄芩药理作用研究述评[J].中华中医药学刊,2009,53(8):1619-1622.
[13]李晨睿,牛银波,潘亚磊,等.黄芩药动学研究进展[J].中国药理学通报,2013,29(8):1048-1053.
[14]刘云波,郭丽华,邱世翠,等.黄芩体外抑菌作用研究[J].时珍国医国药,2002,13(10):596.
[15]肖丽和,王红燕,李发美,等.不同来源黄芩药材HPLC指纹图谱比较[J].沈阳药科大学学报,2004,21(1):28-31.
[16]薛黎明,秦雪梅,张丽增.不同产地黄芩药材的黄芩苷含量测定及指纹图谱研究[J].中成药,2008,30(1):10-13.
[17]常小平,王影.不同产地黄芩中主要有效成分含量比较[J].河南中医,2010,30(12):1176-1177.
[18]宋旦哥.基于不同来源的黄芩物质基础与其药效学相关性研究[D].北京:北京中医药大学,2010.
[19]肖小河,王永炎,刘义.从热力学角度审视和研究中医药[C]//中华中医药学会糖尿病分会.中医药学术发展大会论文集,2005:168-169.
[20]代春美,肖小河,金城,等.微量热分析在中医药研究中的应用[J].中草药,2005,36(10):139-141.
[21]李月生,夏祥翔,李新怀,等.微量热技术在生命科学研究中的应用[J].化工时刊,2007,21(7):64-67.
[22]高誉珊.基于微量量热技术的典型寒热中药生物热动力学研究[D].北京:北京中医药大学,2014.
[23]张萍.肖小河研究员中医药学术思想研究[D].北京:中国人民解放军医学院,2012.
[24]王建涛.中药中有效成分生物活性的微量量热法研究[D].曲阜:曲阜师范大学,2007.
[25]肖小河,金城,鄢丹,等.中药大质量观及实践[J].中草药,2010,41(4):505-508.
[26]赵艳玲,肖小河,刘义.热力学理论在中医药研究中的应用与发展[J].解放军药学学报,2005,21(4):291-293.
[27]李晶,毕红艳,杜保同,等.微量热法测定粉防己碱金属配合物的抑菌性[J].应用化学,2011,28(8):897-900.
[28]刘莲,林贵梅,邵伟,等.微量量热法研究甘草多糖对细菌代谢作用的影响[J].药物生物技术,2011,18(1):48-51.
[29]李晓霞,潘晓茹,吴莉莉,等.麦冬对大肠杆菌代谢作用的微量量热法研究[J].山东中医药大学学报,2001,35(4):307-309.
[30]聂毅,刘晓静,张洪林,等.微量量热法研究草乌生物碱对细菌的代谢作用[J].曲阜师范大学学报(自然科学版),2003,29(1):82-84.
[31]于游,孙玉希,卢海峰,等.微量量热法研究中药麻黄及其有效成分对大肠杆菌的抑制作用[C]//中国药学会药物分析杂志.2003年药物分析论坛“热分析在药物分析中的应用”专题学术研讨会论文集,2003:36.
[32]于游,聂毅,孙玉希,等.微量量热法研究中药茵陈及其有效成分对大肠杆菌的代谢作用[J].曲阜师范大学学报(自然科学版),2004,30(1):72-74.
[33]Zang Q C,Wang J B,Kong W J,et al.Searching for the main anti-bacterial components in artificial Calculus bovis using UPLC and microcalorimetry coupled with multi-linear regression analysis[J].J Sep Sci,2011,34(23):3330-3338.
[34]Kong W,Zhao Y,Shan L,et al.Microcalorimetric studies of the action on four organic acids in Radix isatidis on the growth of microorganisms[J].Sheng Wu Gong Cheng Xue Bao,2008,24(4):646-650.
[2]胡艳军,蒋风雷,欧阳宇,等.微量热法在药物活性评价中的应用[J].中国科学:化学,2010,40(9):1276-1285.
[3]Kong W J,Wang J B,Jin C,et al.Effect of emodin on Candida albicans growth investigated by microcalorimetry combined with chemometric analysis[J].Appl Microbiol Biotechnol,2009,83(6):1183-1190.
[4]赵艳玲,山丽梅,金城,等.基于生物热活性表达的中药板蓝根品质评价研究[J].中药材,2008,31(5):743-747.
[5]马莉,曹俊岭,廖庆文,等.微量量热法对不同产地板蓝根药材品质的评价[J].中国药房,2009,20(30):2324-2326.
[6]张学儒,王伽伯,肖小河,等.大黄药材两种化学型对金葡菌生长代谢热动力学变化的影响[J].药学学报,2010,45(9):1144-1148.
[7]周丹蕾,鄢丹,李宝才,等.微量量热法研究天然虫草和人工虫草对大肠杆菌生长代谢的影响[J].药学学报,2009,44(6):640-644.
[8]王磊,李建宇,袁海龙,等.基于微量量热法的不同种质忍冬质量评价的初步研究[J].解放军药学学报,2010,1(2):133-136.
[9]代春美,肖小河,王迪,等.微量热法对不同生长年份黄连品质的评价[J].中草药,2006,37(2):205-209.
[10]赵艳玲,王伽伯,肖小河.微量热法研究板蓝根的生物热力学特征[J].中草药,2007,38(2):193-196.
[11]张甜甜,张媛媛,陈红鸽,等.基于微量量热法的金银花抗菌活性部位初筛[J].解放军药学学报,2011,7(3):205-207.
[12]宋旦哥,孟庆刚.黄芩药理作用研究述评[J].中华中医药学刊,2009,53(8):1619-1622.
[13]李晨睿,牛银波,潘亚磊,等.黄芩药动学研究进展[J].中国药理学通报,2013,29(8):1048-1053.
[14]刘云波,郭丽华,邱世翠,等.黄芩体外抑菌作用研究[J].时珍国医国药,2002,13(10):596.
[15]肖丽和,王红燕,李发美,等.不同来源黄芩药材HPLC指纹图谱比较[J].沈阳药科大学学报,2004,21(1):28-31.
[16]薛黎明,秦雪梅,张丽增.不同产地黄芩药材的黄芩苷含量测定及指纹图谱研究[J].中成药,2008,30(1):10-13.
[17]常小平,王影.不同产地黄芩中主要有效成分含量比较[J].河南中医,2010,30(12):1176-1177.
[18]宋旦哥.基于不同来源的黄芩物质基础与其药效学相关性研究[D].北京:北京中医药大学,2010.
[19]肖小河,王永炎,刘义.从热力学角度审视和研究中医药[C]//中华中医药学会糖尿病分会.中医药学术发展大会论文集,2005:168-169.
[20]代春美,肖小河,金城,等.微量热分析在中医药研究中的应用[J].中草药,2005,36(10):139-141.
[21]李月生,夏祥翔,李新怀,等.微量热技术在生命科学研究中的应用[J].化工时刊,2007,21(7):64-67.
[22]高誉珊.基于微量量热技术的典型寒热中药生物热动力学研究[D].北京:北京中医药大学,2014.
[23]张萍.肖小河研究员中医药学术思想研究[D].北京:中国人民解放军医学院,2012.
[24]王建涛.中药中有效成分生物活性的微量量热法研究[D].曲阜:曲阜师范大学,2007.
[25]肖小河,金城,鄢丹,等.中药大质量观及实践[J].中草药,2010,41(4):505-508.
[26]赵艳玲,肖小河,刘义.热力学理论在中医药研究中的应用与发展[J].解放军药学学报,2005,21(4):291-293.
[27]李晶,毕红艳,杜保同,等.微量热法测定粉防己碱金属配合物的抑菌性[J].应用化学,2011,28(8):897-900.
[28]刘莲,林贵梅,邵伟,等.微量量热法研究甘草多糖对细菌代谢作用的影响[J].药物生物技术,2011,18(1):48-51.
[29]李晓霞,潘晓茹,吴莉莉,等.麦冬对大肠杆菌代谢作用的微量量热法研究[J].山东中医药大学学报,2001,35(4):307-309.
[30]聂毅,刘晓静,张洪林,等.微量量热法研究草乌生物碱对细菌的代谢作用[J].曲阜师范大学学报(自然科学版),2003,29(1):82-84.
[31]于游,孙玉希,卢海峰,等.微量量热法研究中药麻黄及其有效成分对大肠杆菌的抑制作用[C]//中国药学会药物分析杂志.2003年药物分析论坛“热分析在药物分析中的应用”专题学术研讨会论文集,2003:36.
[32]于游,聂毅,孙玉希,等.微量量热法研究中药茵陈及其有效成分对大肠杆菌的代谢作用[J].曲阜师范大学学报(自然科学版),2004,30(1):72-74.
[33]Zang Q C,Wang J B,Kong W J,et al.Searching for the main anti-bacterial components in artificial Calculus bovis using UPLC and microcalorimetry coupled with multi-linear regression analysis[J].J Sep Sci,2011,34(23):3330-3338.
[34]Kong W,Zhao Y,Shan L,et al.Microcalorimetric studies of the action on four organic acids in Radix isatidis on the growth of microorganisms[J].Sheng Wu Gong Cheng Xue Bao,2008,24(4):646-650.