滋阴养血安神方联合针刺对PCPA诱导的失眠小鼠肝脏TNF-α表达影响
|
摘要
目的 :初步探讨滋阴养血安神方联合针刺对PCPA诱导的失眠小鼠肝脏TNF-α表达的影响。方法:利用腹腔注射对氯苯丙氨酸(PCPA)制造睡眠剥夺模型,分5组,分别为空白对照组、模型组、针刺组(百会穴、神庭穴)、滋阴养血安神方组(中药组)、滋阴养血安神方联合针刺组(针药联合组),造模并给药7 d后,检测小鼠肝TNF-α的mRNA及蛋白表达。结果 :针刺组降低了模型组TNF-α蛋白表达,针药联合组显著降低了TNF-α蛋白表达(P<0.05),针药联合组效果更好。针刺组、针药联合组降低了TNF-α的mRNA表达水平,尤其是针药联合组显著降低了TNF-α的mRNA表达水平(P<0.01),针刺及滋阴养血安神方对降低肝脏TNF-α表达有一定的协同作用。结论 :滋阴养血安神方及针刺法可以影响PCPA诱导的失眠小鼠肝脏TNF-α表达,且针刺与中药具有一定的协同作用。
Objective :To explore the effect of Ziyin Yangxue Anshen Decoction combined with acupuncture on TNF-α expression in the liver of PCPA-induced insomnia mice. Methods :Using the abdominal cavity injection of chlorobenzene alanine(PCPA)to manufacture sleep deprivation model,then 5 group could be devided,blank control group,model group,acupuncture group(Baihui,Shenting),Ziyin Yangxue Anshen Decoction group,Ziyin Yangxue Anshen Decoction combined acupuncture group,after building and delivery 7 days,detection of mice liver TNF-α expression in mRNA and protein level. Results :The acupuncture group reduced the protein level of TNF-α in the model group,especially the acupuncture group and the acupuncture binding drug group significantly reduced the level of TNF-α(P<0.05). The mRNA expression levels of TNF-α were decreased both in the acupuncture group and the combination group,especially in the acupuncture group and the combination group(P<0.01). Acupuncture and Ziyin Yangxue Anshen Decoction had certain synergistic effects on decreasing the expression of TNF-α in liver. Conclusion :Ziyin Yangxue Anshen Decoction combined acupuncture can affect the expression of TNF-α in the liver of insomnia mice induced by PCPA. Synergy of acupuncture and prescription can be seen.
Objective :To explore the effect of Ziyin Yangxue Anshen Decoction combined with acupuncture on TNF-α expression in the liver of PCPA-induced insomnia mice. Methods :Using the abdominal cavity injection of chlorobenzene alanine(PCPA)to manufacture sleep deprivation model,then 5 group could be devided,blank control group,model group,acupuncture group(Baihui,Shenting),Ziyin Yangxue Anshen Decoction group,Ziyin Yangxue Anshen Decoction combined acupuncture group,after building and delivery 7 days,detection of mice liver TNF-α expression in mRNA and protein level. Results :The acupuncture group reduced the protein level of TNF-α in the model group,especially the acupuncture group and the acupuncture binding drug group significantly reduced the level of TNF-α(P<0.05). The mRNA expression levels of TNF-α were decreased both in the acupuncture group and the combination group,especially in the acupuncture group and the combination group(P<0.01). Acupuncture and Ziyin Yangxue Anshen Decoction had certain synergistic effects on decreasing the expression of TNF-α in liver. Conclusion :Ziyin Yangxue Anshen Decoction combined acupuncture can affect the expression of TNF-α in the liver of insomnia mice induced by PCPA. Synergy of acupuncture and prescription can be seen.
引文
[1]黄蓉,黄席珍.睡眠与细胞因子[J].中华老年医学杂志,2002,21(1):7-8.
[2]Deborah Suchecki,Sergio Tufik.Socail stability attenuates the stress in the modified mutiple platform method for paradoxical sleep deprivation in the rat[J].Physiology&Behavior,2000,68:309-316.
[3]Van Leeuen WMA,Lehto M,Karisola P,et al.Sleep restriction increases the risk of developing cardiovascular diseases by augumenting proinflammatory responses through IL-17 and CRP[J].PLoS ONE,2009,4:e4589.
[4]Irwin M,Rinetti G,Redwine L,et al.Nocturnal proinflammatory cytokine-associated sleep disturbances in abstinent African American alcoholices[J].Brain Behav Immun,2004,18:349-360.
[5]Cavadini G,Petrzilka S,Kohler P,et al.TNF-alpha suppresses the expression of clock genes by interfering with Eboxmediated transcription[J].Proc Nat Acad Sci USA,2007,104(31):12843-12848.
[6]肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326.
[7]郑小燕,冉建华,刘刚,等.睡眠剥夺小鼠肝脏中肿瘤坏死因子α表达上调[J].中国组织化学与细胞化学杂志,2017,26(4):354-359.
[8]刘丽君,何菲,郭珮,等.睡眠剥夺上调胰腺TNF-α表达[J].中国组织化学与细胞化学杂志,2015,24(5):461-464.
[9]甘丽,幸宇,陈晓丽,等.慢性睡眠不足诱导小鼠大脑和肝脏氧化应激和炎症反应[J].重庆医科大学学报,2018,43(6):836-842.
[10]季燕,阳文浩,邹志才,等.天王补心丹对伴睡眠障碍的老年痴呆患者血清IL-6、TNF-α的影响[J].中国现代药物应用,2018,12(12):8-10.
[11]程少冰,张毅敏,唐纯志,等.针刺对不同时段睡眠剥夺大鼠模型行为学及TNF-α含量的影响[J].中国老年学杂志,2012(1):77-79.
[12]胡天俊,虞杰,王秀莲,等.电针对睡眠剥夺大鼠行为学和IL-1β、IL-6和TNF-α的影响[J].天津中医药,2016,33(1):35-38.
[13]Toth LA,Hughes LF.Macrophage participation in influenzainduced sleep enhancement in C57BL/6J mice[J].Brain Behav Immun,2004,18:375-389.
[14]李洋,谢杨丽,胡志安.感染和免疫反应对睡眠的影响[J].生理学科学进展,2007,38(2):187-190.
[15]张颖,齐越,吴怡,等.枣仁安神颗粒改善睡眠作用的量效、时效关系及对脑内细胞因子的影响[J].中国药业,2015,24(2):32-34.
[16]Fu Q,Yuan HM,Chen J,et al.Dammarane-type saponins from Ziziphsjujube,and their inhibitory effects against TNF-αrelease in LPS-induced RAW 246.7 macrophage[J].Phytochen Lett,2016(16):169-173.
[17]Yuan Y,Wu S,Li Z,et al.Wild jujube polysaccharides protect against experimental inflammatory bowel disease by enabling enhanced intestinal barrier funcetion[J].Food Funct,2015,6(8):2568-2577.
[2]Deborah Suchecki,Sergio Tufik.Socail stability attenuates the stress in the modified mutiple platform method for paradoxical sleep deprivation in the rat[J].Physiology&Behavior,2000,68:309-316.
[3]Van Leeuen WMA,Lehto M,Karisola P,et al.Sleep restriction increases the risk of developing cardiovascular diseases by augumenting proinflammatory responses through IL-17 and CRP[J].PLoS ONE,2009,4:e4589.
[4]Irwin M,Rinetti G,Redwine L,et al.Nocturnal proinflammatory cytokine-associated sleep disturbances in abstinent African American alcoholices[J].Brain Behav Immun,2004,18:349-360.
[5]Cavadini G,Petrzilka S,Kohler P,et al.TNF-alpha suppresses the expression of clock genes by interfering with Eboxmediated transcription[J].Proc Nat Acad Sci USA,2007,104(31):12843-12848.
[6]肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326.
[7]郑小燕,冉建华,刘刚,等.睡眠剥夺小鼠肝脏中肿瘤坏死因子α表达上调[J].中国组织化学与细胞化学杂志,2017,26(4):354-359.
[8]刘丽君,何菲,郭珮,等.睡眠剥夺上调胰腺TNF-α表达[J].中国组织化学与细胞化学杂志,2015,24(5):461-464.
[9]甘丽,幸宇,陈晓丽,等.慢性睡眠不足诱导小鼠大脑和肝脏氧化应激和炎症反应[J].重庆医科大学学报,2018,43(6):836-842.
[10]季燕,阳文浩,邹志才,等.天王补心丹对伴睡眠障碍的老年痴呆患者血清IL-6、TNF-α的影响[J].中国现代药物应用,2018,12(12):8-10.
[11]程少冰,张毅敏,唐纯志,等.针刺对不同时段睡眠剥夺大鼠模型行为学及TNF-α含量的影响[J].中国老年学杂志,2012(1):77-79.
[12]胡天俊,虞杰,王秀莲,等.电针对睡眠剥夺大鼠行为学和IL-1β、IL-6和TNF-α的影响[J].天津中医药,2016,33(1):35-38.
[13]Toth LA,Hughes LF.Macrophage participation in influenzainduced sleep enhancement in C57BL/6J mice[J].Brain Behav Immun,2004,18:375-389.
[14]李洋,谢杨丽,胡志安.感染和免疫反应对睡眠的影响[J].生理学科学进展,2007,38(2):187-190.
[15]张颖,齐越,吴怡,等.枣仁安神颗粒改善睡眠作用的量效、时效关系及对脑内细胞因子的影响[J].中国药业,2015,24(2):32-34.
[16]Fu Q,Yuan HM,Chen J,et al.Dammarane-type saponins from Ziziphsjujube,and their inhibitory effects against TNF-αrelease in LPS-induced RAW 246.7 macrophage[J].Phytochen Lett,2016(16):169-173.
[17]Yuan Y,Wu S,Li Z,et al.Wild jujube polysaccharides protect against experimental inflammatory bowel disease by enabling enhanced intestinal barrier funcetion[J].Food Funct,2015,6(8):2568-2577.