中国三种不同地方品系鸡对J亚群禽白血病病毒NX0101株易感性的比较
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摘要
为了研究不同中国地方品系鸡对J亚群禽白血病病毒(subgroup J Avian leukosis virus,ALV-J)的易感性和抵抗力,将ALV-J中国分离株NX0101分别通过鸡胚静脉和卵黄囊两种接种方式接种莱芜黑鸡、汶上芦花鸡、济宁百日鸡3种不同地方品系鸡,分析比较NX0101株对3种品系鸡的致病性。结果表明,NX0101株均可诱发3种品系鸡的髓样细胞瘤,并引起生长迟缓和免疫抑制等亚临床症状,但致病性的强度差异具有显著统计学意义。在两种不同接种方式下,莱芜黑鸡的死亡率和肿瘤发生率均明显低于另外两个品系,并且感染莱芜黑鸡的体重受抑制程度最低。济宁百日鸡对NX0101株的抵抗力在这3种品系鸡中最弱。因此,莱芜黑鸡可以作为ALV-J遗传抗性基因筛选和抗病育种的候选品系。
To investigate the susceptibility and resistance of different Chinese indigenous chickens to subgroup J Avian leukosis virus(ALV-J) infection, three breeds(Laiwu Black, Wenshang Barred and Jining Bairi) were inoculated with ALV-J Chinese isolate NX0101 strain via intravenous and yolk sac routes. Several parameters including viremia, antibody response to viral infection and chicken growth were observed and analyzed. Results demonstrated that NX0101 infection of three different indigenous chicken breeds developed myelocytoma and showed subclinical traits, such as growth retardation and immunosuppression. However, pathogenicity of NX0101 varied greatly in different breeds. The mortality and tumor incidence of Laiwu Black chickens infected with NX0101 through intravenous or yolk sac were significantly lower than those of other breeds. In addition, the inhibition ratio of weight growth of Laiwu Black was the lowest in three breeds. Therefore, Laiwu Black chickens would be used as the candidate line to screen genetic resistance genes for ALV-J and further investigate genetic mechanism.
To investigate the susceptibility and resistance of different Chinese indigenous chickens to subgroup J Avian leukosis virus(ALV-J) infection, three breeds(Laiwu Black, Wenshang Barred and Jining Bairi) were inoculated with ALV-J Chinese isolate NX0101 strain via intravenous and yolk sac routes. Several parameters including viremia, antibody response to viral infection and chicken growth were observed and analyzed. Results demonstrated that NX0101 infection of three different indigenous chicken breeds developed myelocytoma and showed subclinical traits, such as growth retardation and immunosuppression. However, pathogenicity of NX0101 varied greatly in different breeds. The mortality and tumor incidence of Laiwu Black chickens infected with NX0101 through intravenous or yolk sac were significantly lower than those of other breeds. In addition, the inhibition ratio of weight growth of Laiwu Black was the lowest in three breeds. Therefore, Laiwu Black chickens would be used as the candidate line to screen genetic resistance genes for ALV-J and further investigate genetic mechanism.
引文
[1]Fadly A M,Payne L N.Leukosis/Sarcoma group.Diseases of poultry[M].Ames:Lowa State University Press.2008:514-681.
[2]Payne L N,Nair V.The long view:40 years of avian leukosis research[J].Avian Pathol,2012,41(1):11-19.
[3]Payne L N.HPRS-103:a retrovirus strikes back.The emergence of subgroup J avian leukosis virus[J].Avian Pathol,1998,27:S36-S45.
[4]杜岩,崔治中,秦爱建,等.从市场商品肉鸡中检出J亚群禽白血病病毒[J].中国家禽学报,1999,3(1):1-4.
[5]杜岩,崔治中,秦爱建,等.鸡的J亚群白血病病毒的分离及部分序列比较[J].病毒学报,2000,16(4):341-346.
[6]成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45(4):584-587.
[7]Zhao P,Cui Z,Ma C,et al.Serological survey of the avainleukosisvirus infection in China native chickens[J].J Anim Vet Adv,2012,11(14):2584-2587.
[8]崔治中.我国鸡群J亚群白血病流行的过去、现在和将来及其防控——对我国动物疫病防控的启示[J].现代畜牧兽医,2015(1):30-32.
[9]Wang Y,Xu S,Li S,et al.Lamivudine inhibits the replication of ALV-J associated acutely transforming virus and its helper virus and tumor growth in vitro and in vivo[J].Front Microbiol,2015,6:1306.
[10]崔治中.种源净化是实现家禽规模化养殖业生物安全最基本的核心措施[J].中国家禽,2012(2):4-8.
[11]崔治中,孙淑红,赵鹏,等.对种鸡场禽白血病净化方案的建议[J].中国家禽,2014,36(1):3-6.
[12]刘将军,袁峥嵘,何俊.抗病育种在畜禽育种中的应用[J].中国畜牧兽医,2008,35(4):62-64.
[13]Crittenden L B,Motta J V.A survey of genetic resistance to leukosis-sarcoma viruses in commercial stocks of chickens[J].Poultry Sci,1969,48(5):1751-1757.
[14]Motta J V,Crittenden L B,Pollard W O.The inheritance of resistance to subgroup C leukosis-sarcoma viruses in New Hampshire chickens[J].Poultry Sci,1973,52(2):578-586.
[15]Bacon L D,Fulton J E,Kulkarni G B.Methods for evaluating and developing commercial chicken strains free of endogenous subgroup E avian leukosis virus[J].Avian Pathol,2004,33(2):233-243.
[16]Chen W,Liu Y,Li H,et al.Intronic deletions of tva receptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].Sci Rep,2015,5:9900.
[17]张纪元,崔治中,丁家波,等.J亚群白血病病毒NX0101株感染性克隆化病毒的构建及其生物学特性[D].泰安:山东农业大学,2005.
[18]崔治中.中国鸡群病毒性肿瘤病及防控研究[M].北京:中国农业出版社.2013,36-48.
[19]杜岩,崔治中.J亚群禽白血病病毒中国分离株的人工致病性试验[J].中国农业科学,2002,35(4):430-433.
[20]Cui Z,Sun S,Wang J.Reduced serologic response to Newcastle disease virus in broiler chickens exposed to a Chinese field strain of subgroup J avian leukosisvirus[J].Avian Dis,2006,50(2):191-195.
[21]张利,刘青,王晓伟,等.禽白血病病毒J亚群(ALV-J)免疫抑制特性研究[J].畜牧兽医学报,2010,41(9):1198-1202.
[22]康相涛,田亚东,李孝法,等.中国地方鸡种质资源优异性状发掘创新与应用[J].中国家禽,2009,31(4):1-5.
[2]Payne L N,Nair V.The long view:40 years of avian leukosis research[J].Avian Pathol,2012,41(1):11-19.
[3]Payne L N.HPRS-103:a retrovirus strikes back.The emergence of subgroup J avian leukosis virus[J].Avian Pathol,1998,27:S36-S45.
[4]杜岩,崔治中,秦爱建,等.从市场商品肉鸡中检出J亚群禽白血病病毒[J].中国家禽学报,1999,3(1):1-4.
[5]杜岩,崔治中,秦爱建,等.鸡的J亚群白血病病毒的分离及部分序列比较[J].病毒学报,2000,16(4):341-346.
[6]成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45(4):584-587.
[7]Zhao P,Cui Z,Ma C,et al.Serological survey of the avainleukosisvirus infection in China native chickens[J].J Anim Vet Adv,2012,11(14):2584-2587.
[8]崔治中.我国鸡群J亚群白血病流行的过去、现在和将来及其防控——对我国动物疫病防控的启示[J].现代畜牧兽医,2015(1):30-32.
[9]Wang Y,Xu S,Li S,et al.Lamivudine inhibits the replication of ALV-J associated acutely transforming virus and its helper virus and tumor growth in vitro and in vivo[J].Front Microbiol,2015,6:1306.
[10]崔治中.种源净化是实现家禽规模化养殖业生物安全最基本的核心措施[J].中国家禽,2012(2):4-8.
[11]崔治中,孙淑红,赵鹏,等.对种鸡场禽白血病净化方案的建议[J].中国家禽,2014,36(1):3-6.
[12]刘将军,袁峥嵘,何俊.抗病育种在畜禽育种中的应用[J].中国畜牧兽医,2008,35(4):62-64.
[13]Crittenden L B,Motta J V.A survey of genetic resistance to leukosis-sarcoma viruses in commercial stocks of chickens[J].Poultry Sci,1969,48(5):1751-1757.
[14]Motta J V,Crittenden L B,Pollard W O.The inheritance of resistance to subgroup C leukosis-sarcoma viruses in New Hampshire chickens[J].Poultry Sci,1973,52(2):578-586.
[15]Bacon L D,Fulton J E,Kulkarni G B.Methods for evaluating and developing commercial chicken strains free of endogenous subgroup E avian leukosis virus[J].Avian Pathol,2004,33(2):233-243.
[16]Chen W,Liu Y,Li H,et al.Intronic deletions of tva receptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].Sci Rep,2015,5:9900.
[17]张纪元,崔治中,丁家波,等.J亚群白血病病毒NX0101株感染性克隆化病毒的构建及其生物学特性[D].泰安:山东农业大学,2005.
[18]崔治中.中国鸡群病毒性肿瘤病及防控研究[M].北京:中国农业出版社.2013,36-48.
[19]杜岩,崔治中.J亚群禽白血病病毒中国分离株的人工致病性试验[J].中国农业科学,2002,35(4):430-433.
[20]Cui Z,Sun S,Wang J.Reduced serologic response to Newcastle disease virus in broiler chickens exposed to a Chinese field strain of subgroup J avian leukosisvirus[J].Avian Dis,2006,50(2):191-195.
[21]张利,刘青,王晓伟,等.禽白血病病毒J亚群(ALV-J)免疫抑制特性研究[J].畜牧兽医学报,2010,41(9):1198-1202.
[22]康相涛,田亚东,李孝法,等.中国地方鸡种质资源优异性状发掘创新与应用[J].中国家禽,2009,31(4):1-5.