丹参中丹酚酸B对脑缺血再灌注损伤大鼠的保护作用研究
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摘要
目的:观察丹酚酸B对脑缺血再灌注损伤大鼠的保护作用。方法:SD大鼠随机分为假手术组,模型组,丹酚酸B组,尼莫地平组,线栓法制作大鼠大脑中动脉局灶性栓塞(MCAO)模型,观察对损伤后大鼠的神经功能,脑梗死体积,脑含水量、脑指数,缺血侧脑组织SOD、CAT、GSH-Px活力,MDA含量,LDH、NOS活力等的影响。结果:丹酚酸B可显著地减轻MCAO模型大鼠的神经功能缺损症状,促进神经功能缺损症状的恢复;显著地缩小大鼠MCAO后脑组织梗死体积,降低脑梗死体积百分率;降低脑含水量和脑指数;显著地提高缺血再灌注后大鼠脑组织中SOD、CAT、GSH-Px等的活力,明显降低MDA含量,降低LDH、NOS等的活力。结论:丹酚酸B对大鼠脑缺血性损伤具有明显的抗脑水肿、抗氧化作用而起到脑保护作用。
Objective:To observe the protective effects of Salvianolic acid B on cerebral ischemia and reperfusion injury in rats. Methods:The SD rats were randomly divided into sham-operated group, model group,Salvianolic acid B group and nimodipine group.Using focal middle cerebral artery embolization(MCAO) model,we observedthe targets including symptoms of neurological deficit score,infarct volume,brain index and brain water contents,activitives of SOD,CAT,GSH-Px,LDH and NOS,and the content of MDA of ischemic brain tissue. Results: Salvianolic acid B could significantly reduce the symptoms of neurological deficits, promote the recovery symptoms of neurological deficits,narrow infarct volume of brain tissue obviously, reduce brain index and brain water,raise the activitives of SOD,CAT and GSH-Px, and reduce the content of MDA and the activitives of LDH and NOS. Conclusions:Salvianolic acid B has significant protective effects against brain edema andantioxidation.
Objective:To observe the protective effects of Salvianolic acid B on cerebral ischemia and reperfusion injury in rats. Methods:The SD rats were randomly divided into sham-operated group, model group,Salvianolic acid B group and nimodipine group.Using focal middle cerebral artery embolization(MCAO) model,we observedthe targets including symptoms of neurological deficit score,infarct volume,brain index and brain water contents,activitives of SOD,CAT,GSH-Px,LDH and NOS,and the content of MDA of ischemic brain tissue. Results: Salvianolic acid B could significantly reduce the symptoms of neurological deficits, promote the recovery symptoms of neurological deficits,narrow infarct volume of brain tissue obviously, reduce brain index and brain water,raise the activitives of SOD,CAT and GSH-Px, and reduce the content of MDA and the activitives of LDH and NOS. Conclusions:Salvianolic acid B has significant protective effects against brain edema andantioxidation.
引文
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[20] 丁彩娟,江勤,王鑫,等.补阳还五汤有效部位对动物全脑缺血损伤的保护作用及抗氧化与凋亡机制[J].中药药理与临床,2017,33(4):2-7.
[21] 颜娟,郑茂东,崔玉环,等.大黄酚脂质体对脑缺血再灌注损伤小鼠体内抗氧化作用的影响[J].中国老年学杂志,2018,38(3):703-704.
[22] 方晓艳,郑艳,贾佼佼,等.脑缺血预处理对脑缺血再灌注损伤大鼠抗氧化酶的影响[J].中药药理与临床,2016,32(3):193-196.
[2] 王瑞芳,李月玲,杨玉梅.中药复方制剂抗缺血性脑损伤作用机制研究现状与进展[J].包头医学院学报,2018(5):121-123.
[3] 国家药典委员会.中华人民共和国药典:一部[S].北京:中国中医药出版社,2015:76-77.
[4] 林超,刘兆国,钱星,等.丹酚酸B在心血管疾病中药理作用研究进展[J].中国药理学通报,2015,31(4):449-452.
[5] 赵先,王婧雯,陆杨,等.丹酚酸B药理作用的研究进展[J].西北药学杂志,2015,30(1):107-110.
[6] 柴媛媛,王芳,李艳丽,等.两种丹酚酸的体外抗氧化活性比较研究[J].时珍国医国药,2012,23(11):2681-2683.
[7] 丹方蕾,杨美娟,蒋玉凤.酚酸B对脑缺血再灌注损伤小鼠血管保护作用[J].中华中医药学刊,2016,34(12):3060-3062.
[8] Ludmila Belayev,Ofelia FA,Raul B,et al.Middle Cerebral Cortery Occlusion in the rat by intraluminal suture[J].Stroke,1996,27(9):1612-1616.
[9] Longa EZ,Weistein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[10] YU YP,XU QQ,ZHENG MZ,et al.Light transmission measurement of focal ischemic cerebral infarction in mice [J].Journal of Zhejiang Unviersity (Medical Sciences),2002,31(2):91-93.
[11] 房亚兰,尹洁,刘克建,等.脑缺血再灌注损伤中自由基与锌离子的相互作用[J].首都医科大学学报,2018,39(3):373-377.
[12] Ashwal S,Tone B,Tian HR,et al.Core and penumbral nitric oxide synthase activity during cerebral ischemia and reperfusion[J].Stroke,1998,29(5):1037-1047.
[13] 刘永刚,王婧,李芳君,等.白藜芦醇对脑缺血-再灌注损伤大鼠脑组织及血清中NO 含量及NOS、iNOS 活性及黏附分子的影响[J].中药材,2010,33(10):1609-1611.
[14] Wang K,Chen Z,Huang J,et al.Naringenin prevents ischaemic stroke damage via anti-apoptotic and anti-oxidant effects[J].Clin Exp Pharmacol Physiol,2017,44(8):862-871.
[15] 陈丹丹,谢晓芳,李梦婷,等.氢溴酸樟柳碱对慢性脑缺血损伤大鼠氧化应激及细胞凋亡的影响[J].中成药,2018,40(6):1242-1248
[16] 梁沛余,段路路,吴穹.藏药十八味诃子利尿丸对大鼠局灶性脑缺血/再灌注损伤的保护作用[J].中国老年学杂志,2018,38(16):3973-3975.
[17] 洪浩,刘国卿.缺血性脑血管疾病治疗的抗氧化应激策略[J].中国药理学通报,2004 (1):19-24.
[18] 王富明,徐虹,孙华.脑缺血再灌注损伤治疗的实验研究进展[J].医学研究杂志,2015,44(2):12-15.
[19] 乔玉玲.NRG-1β干预抗氧化酶SOD、NQO1在脑出血大鼠血肿周围表达及作用机制研究[D].青岛:青岛大学,2017.
[20] 丁彩娟,江勤,王鑫,等.补阳还五汤有效部位对动物全脑缺血损伤的保护作用及抗氧化与凋亡机制[J].中药药理与临床,2017,33(4):2-7.
[21] 颜娟,郑茂东,崔玉环,等.大黄酚脂质体对脑缺血再灌注损伤小鼠体内抗氧化作用的影响[J].中国老年学杂志,2018,38(3):703-704.
[22] 方晓艳,郑艳,贾佼佼,等.脑缺血预处理对脑缺血再灌注损伤大鼠抗氧化酶的影响[J].中药药理与临床,2016,32(3):193-196.