血清对Ⅱ型脊髓灰质炎病毒感染性滴度检测结果的影响
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摘要
目的比较6种不同血清对Ⅱ型脊髓灰质炎病毒感染性滴度(CCID50)检测结果的影响。方法分别采用6种未处理血清和6种预处理血清组(免疫吸附法)进行Ⅱ型脊髓灰质炎病毒感染性滴度检测(微细胞病变法),检测了不同组血清中Ⅱ型脊髓灰质炎病毒中和抗体滴度,并使用处理血清组进行促细胞生长试验。最后分析不同血清对感染性滴度的影响。结果6种未处理血清组病毒感染性滴度检测结果差异有统计学意义(P<0.01),6种处理血清组病毒感染性滴度差异无统计学意义(P>0.05)。未处理组2号血清的中和抗体滴度为1∶4,6号血清的中和抗体滴度为1∶8,其余各组血清的中和抗体滴度均<1∶4。6种处理血清组的中和抗体滴度均为1∶2。血清促细胞生长试验显示6种处理组血清细胞生长趋势正常。结论Ⅱ型脊髓灰质炎病毒感染性滴度检测前使用免疫吸附对血清进行预处理,可降低血清中存在的中和抗体对检测的影响,为比较不同实验室数据提供可能。
Objective To compare 6 different serum' s influence on type Ⅱ poliovirus tissue culture median infective dose(CCID50).Methods We detected the neutral antibody titer of type Ⅱ poliovirus using 6 original serum and 6 predisposed serum(immunoadsorption method).At the same time,we also performed cell growth experiment using predisposed serum.In addition,we analyzed the data to find out different serum's influence on type Ⅱ poliovirus CCID50.Results There is a significant difference among the type Ⅱpoliovirus CCID50 results of the 6 original serum groups.However,an opposite result had been shown in those predisposed serum groups.In addition,we noticed that the neutral antibody titer of type Ⅱ poliovirus in original serum groups is 1∶ 2 except No.2 group with 1∶ 4.The No.6 predisposed serum group had a 1∶ 8 neutral antibody titer but the other 5 predisposed serum groups share a 1∶ 2 neutral antibody titer.Furthermore,these cells had a normal growth tendency in the 6 predisposed serum groups.Conclusion The neutralizing antibody's influence on type Ⅱ poliovirus CCID50 test can be reduced by predisposing serum through immunoadsorption method,which provides the possibility for different laboratories' data comparison.
Objective To compare 6 different serum' s influence on type Ⅱ poliovirus tissue culture median infective dose(CCID50).Methods We detected the neutral antibody titer of type Ⅱ poliovirus using 6 original serum and 6 predisposed serum(immunoadsorption method).At the same time,we also performed cell growth experiment using predisposed serum.In addition,we analyzed the data to find out different serum's influence on type Ⅱ poliovirus CCID50.Results There is a significant difference among the type Ⅱpoliovirus CCID50 results of the 6 original serum groups.However,an opposite result had been shown in those predisposed serum groups.In addition,we noticed that the neutral antibody titer of type Ⅱ poliovirus in original serum groups is 1∶ 2 except No.2 group with 1∶ 4.The No.6 predisposed serum group had a 1∶ 8 neutral antibody titer but the other 5 predisposed serum groups share a 1∶ 2 neutral antibody titer.Furthermore,these cells had a normal growth tendency in the 6 predisposed serum groups.Conclusion The neutralizing antibody's influence on type Ⅱ poliovirus CCID50 test can be reduced by predisposing serum through immunoadsorption method,which provides the possibility for different laboratories' data comparison.
引文
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12 Walker AT,Williams AJ,Gary HE Jr,et al.Effect of time at temperature on wild poliovirus titers in stool specimens[J].Virology,2015,428:28-31
2 赵玉秀,李爱灵,梁宏阳,等.Sabin株脊髓灰质炎灭活疫苗在动物体内的免疫原性及安全性评价[J].中国生物制品学杂志,2015,28(7):665-669
3 Andreasen LV,Hansen LB,Andersen P,et al.Aluminium.hydroxide potentiates a protective Th1 biased immune response against polio virus that allows for dose sparing?in mice and rats[J].Vaccine,2015,33:1873-1879
4 谢忠平,宋霞,崔萍芳,等.OPV疫苗病毒感染性滴度检测的影响因素分析[J].中国公共卫生,2005,21(7):818-819
5 宋冬梅,张中洋,鲁卫卫,等.Sabin株脊髓灰质炎灭活疫苗免疫原性研究[J].中国新药杂志,2015,24(8):878-883
6 王芳,张名,谢炳锋,等.铝佐剂和免疫程序对Sabin IPV免疫原性研究[J].中华实验和临床病毒学杂志,2013,27(2):102-104
7 张香玲.Vero细胞无血清培养基的研究进展[J].微生物学免疫学进展,2015,43(2):67-72
8 戴永祥,李克坚,王明秀,等.用烛斑法和微滴法滴定脊髓灰质炎病毒的比较[J].中华实验和临床病毒学杂志,1994,8(3):268
9 张金菊,张合润,何莹.血清试剂对脊髓灰质炎病毒滴度影响的比较研究[J].中国卫生检验杂志,2007,17(9):1647-1648
10 孙招金,陈柄企,王玲,等.不同新生牛血清对体外培养BHK-21细胞的影响[J].中国生物制品学杂志,2012,25(7):862-864
11 刘萍,马全英,宋玉霞,等.不同牛血清促悬浮培养BHK21细胞生长的作用[J].安徽农业科学,2016,44(2):177-178,199
12 Walker AT,Williams AJ,Gary HE Jr,et al.Effect of time at temperature on wild poliovirus titers in stool specimens[J].Virology,2015,428:28-31